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pC0052-REPAIR non-targeting guide clone into pC0043

Plasmid

#103868

Purpose

Non-targeting guide RNA for use with PspCas13b. Useful as a control for RNA editing and RNA knockdown experiments.

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

non-targeting guide RNA

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA1786

Plasmid

#240489

Purpose

CRISPRi-mediated Knockdown of RPA1786

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pX330_DDX4-cterm

Plasmid

#222520

Purpose

Cas9/sgRNA plasmid for targeting DDX4

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

Cas9, DDX4 sgRNA (DDX4 Human, Synthetic)

Use

CRISPR

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pMD18T-AtU6-HV5

Plasmid

#239470

Purpose

Encodes a gRNA expression cassette driven by the AtU6 promoter.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

U6-gRNA

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMD18T-AtU3b-HV5

Plasmid

#239477

Purpose

Encodes a gRNA expression cassette driven by the AtU3b promoter.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

U3b-gRNA

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMD18T-At7SL-HV5

Plasmid

#239478

Purpose

Encodes a gRNA expression cassette driven by the At7SL promoter.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

7SL-gRNA

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pADH100

Plasmid

#90980

Purpose

NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Insert

NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, FRT, DS_HIS1

Use

CRISPR

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB5191

Plasmid

#126911

Purpose

Plasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiae

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

X-4 and XII-5 gRNA

Use

CRISPR

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLIP-puro-dsRed-GFP-miRNA

Plasmid

#32704

Depositor

Hans Clevers

Article

Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

Insert

emGFP-miRNA

Use

Cre/Lox, RNAi, and Retroviral

Expression

Mammalian

Available Since

Dec. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pLH-spsgRNA2

Plasmid

#64114

Purpose

Vector for expression of Sp sgRNA2 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp sgRNA2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-sgMaob-HepmCherry

Plasmid

#192829

Purpose

Expresses sgRNA targeting Maob and mCherry from hepatocyte-specific promoter

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 for sgRNA and hepatocyte-specific promoter (HS…

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pFH6_new

Plasmid

#105866

Purpose

more efficient version of pFH6; Subcloning of any sgRNA via BbsI site

Depositor

Andreas Weber

Article

Hahn et al Bio Protoc. 2017 Jul 5;7(13):e2384. doi:

Insert

U6-26p::sgRNA scaffold

Use

CRISPR and Synthetic Biology; Subcloning

Expression

Plant

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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SS9_RNA

Plasmid

#71656

Purpose

SS9-targeting gRNA for co-transformation with pSS9

Depositor

Ryan Gill

Article

Bassalo et al ACS Synth Biol. 2016 Apr 22.

Insert

SS9 gRNA

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSIL-eGFP

Plasmid

#52675

Purpose

shRNA silencing plasmid with eGFP transfection marker

Depositor

Johannes Hell

Article

Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

Type

Empty backbone

Use

RNAi

Tags

CMV-EGFP

Promoter

CMV and U6

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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eGFP L202 gRNA

Plasmid

#119132

Purpose

gRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

eGFP L202 gRNA

Use

CRISPR

Mutation

None

Promoter

U6

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_class_I_1

Plasmid

#164986

Purpose

Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA class I

Use

CRISPR

Expression

Mammalian

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g3

Plasmid

#80786

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 3/13/23 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pADH119

Plasmid

#90985

Purpose

NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb LEUpOUT CRISPR system.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Insert

gRNA conserved, C. albicans LEU2 2 of 2

Use

CRISPR

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g1

Plasmid

#80784

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 1/11/21 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g4

Plasmid

#80787

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 4/14/24 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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