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20,047 results

Showing: 3261 - 3280 of 20047 results

  2. pMH-SFB

    Plasmid
    #99391
    Purpose
    Gateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)
    Depositor
    Michael Huen
    Article
    An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114
    Type
    Empty backbone
    Tags
    SFB (S protein-FLAG-Streptavidin binding peptide)
    Expression
    Mammalian
    Available Since
    Sept. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    99391-plasmid-map-sequence-id-194202

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  4. pET His6 MBP TEV LIC cloning vector (1M)

    Plasmid
    #29656
    Depositor
    Scott Gradia
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    6xHis, MBP, and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    Aug. 17, 2011
    Availability
    Academic Institutions and Nonprofits only
    29656-plasmid-map-sequence-id-237947

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  11. PB53x EF1-Dendra2-RPB1Amr

    Plasmid
    #81228
    Purpose
    expresses Dendra2-Rpb1 fusion in mammalian cells. Possible to insert into the genome using the Piggibac system
    Depositor
    Timothée Lionnet
    Article
    Cho et al Elife. 2016 May 3;5. pii: e13617. doi: 1
    Insert
    Dendra2 - Rpb1 (alpha amanitin resistant) (POLR2A Human)
    Tags
    Dendra2
    Expression
    Mammalian
    Mutation
    N792D alpha amanitin resistance mutation (Bartolo…
    Promoter
    EF1a
    Available Since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    81228-plasmid-map-sequence-id-394051

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  12. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid
    #185912
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8e-nSpRY-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185912-plasmid-map-sequence-id-367362

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  14. pGL4.10/RSV_SF3B3-miniXon_Luciferase

    Plasmid
    #174660
    Purpose
    Plasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.
    Depositor
    Beverly L. Davidson
    Article
    Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586
    Insert
    miniXon cassette
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Kozak ATG initiation sequence in middle exon
    Promoter
    RSV
    Available Since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    174660-plasmid-map-sequence-id-351622

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  17. pENN.AAV.cTNT.PI.eGFP.WPRE.rBG

    Plasmid
    #105543
    Purpose
    AAV expression of EGFP from cTNT promoter
    Depositor
    James M. Wilson
    Has Service
    AAV9
    Insert
    cTNT-PI-EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    cTNT
    Available Since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105543-plasmid-map-sequence-id-202437

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  20. pLenti-sgRNA

    Plasmid
    #71409
    Purpose
    There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI
    Depositor
    Eric Lander, David Sabatini
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71409-plasmid-map-sequence-id-322475

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Showing: 3261 - 3280 of 20047 results