We narrowed to 12,329 results for: pcdna

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pCDNA3.1-Flag-H2A K13R

Plasmid

#63566

Purpose

Expression of human H2A with K13R tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine at 13 is now Arginine

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-Flag-H2A K15R

Plasmid

#63567

Purpose

Expression of human H2A with K15R tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine at 15 is now Arginine

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-NBid-PhoCl1-CBid

Plasmid

#164050

Purpose

Optogenetic manipulation of cell apoptosis using PhoCl1

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NBid-PhoCl1-CBid

Tags

kozak sequence

Expression

Mammalian

Promoter

CMV promotor

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO MycLAP-STIL 5A

Plasmid

#80267

Purpose

mammalian expression plasmid for MycLAP-STIL 5A

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

STIL (STIL Human)

Tags

Myc-GFP

Expression

Mammalian

Mutation

S1103A, S1108A, S1111A, S1116A, T1119A

Promoter

CMV

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TAT-2-130-FLAG

Plasmid

#35984

Depositor

Susana Valente

Article

Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

Insert

transactivating protein (tat )

Tags

Flag

Expression

Mammalian

Mutation

tat from HIV2 130 amino acids

Available Since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3-MARS-p85(iSH2)

Plasmid

#205236

Purpose

Expresses PLEKAH5 aa 143-271 (K163A and R164A) fused to the iSH2 domain of mouse p85beta

Depositor

Jeremy Baskin

Article

Cao et al Nat Cell Biol. 2024 Oct;26(10):1804-1816

Inserts

PLEKHA5(143-271)-K163A/R164A (PLEKHA5 Human)
p85(iSH2) (Pik3r1 Mouse)

Tags

Nuclear Export Sequence and mCherry

Expression

Mammalian

Mutation

amino acids 143-271 of PLEKHA5 (GenBank reference…

Promoter

CMV

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HCMVgO-sfGFP (Merlin)

Plasmid

#136452

Purpose

Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) fused to superfolder GFP

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein O (UL74 Human betaherpesvirus 5)

Tags

Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HCMVgL-FLAG (Merlin)

Plasmid

#136450

Purpose

Mammalian expression plasmid for HCMV glycoprotein L (Merlin strain) with FLAG tag

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein L (UL115 Human betaherpesvirus 5)

Tags

Short SGGSG linker and FLAG tag

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-p110α-CAAX

Plasmid

#39490

Depositor

Julian Downward

Article

Rodriguez-Viciana et al EMBO J. 1996 May 15;15(10):2442-51.

Insert

p110 (PIK3CA Bovine)

Tags

CAAX and myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 hsa-mir-214

Plasmid

#26313

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-214 (MIR214 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag-YOD1 C160S

Plasmid

#49785

Purpose

Express YOD1 C160S mutant with a N-terminal Flag-tag in mammalian cells

Depositor

Christian Schlieker

Article

Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

Insert

YOD1 (YOD1 Human)

Tags

Flag-tag

Mutation

C160S

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA-f:PGC1(delta CTD)

Plasmid

#1030

Depositor

Bruce Spiegelman

Article

Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

Insert

PGC1a (Ppargc1a Mouse)

Tags

Flag

Expression

Mammalian

Mutation

delta CTD

Available Since

Oct. 4, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FRB-LplA(W37V)

Plasmid

#35608

Depositor

Alice Ting

Article

Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

Insert

FRB-LplA(W37V)

Tags

FLAG (internal fusion), HA, and His Tag

Expression

Mammalian

Mutation

LplA has Trp37 to Val mutation

Promoter

CMV

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-142

Plasmid

#26309

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-142 (MIR142 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNAintron-CHIKV-nsp2-c3xFLAG

Plasmid

#215695

Purpose

Produces the Chikungunya virus nonstructural protein 2 with a c-terminal 3xFLAG tag

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV nsp2

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNAintron-CHIKV-nsp4-c3xFLAG

Plasmid

#215697

Purpose

Produces the Chikungunya virus nonstructural protein 4 with a c-terminal 3xFLAG tag

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV nsp4

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNAintron-CHIKV-nsp1-c3xFLAG

Plasmid

#215694

Purpose

Produces the Chikungunya virus nonstructural protein 1 with a c-terminal 3xFLAG tag

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV nsp1

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3_DRD1-NTEV-TCS-GV-2xHA

Plasmid

#194358

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

DRD1-NTEV-TCS-GV-2xHA (DRD1 Synthetic, Human)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3 -5'cMyc-SMCl- S957A

Plasmid

#32368

Depositor

Michael Kastan

Article

Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

Insert

SMC1A (SMC1A Human)

Tags

c-Myc

Expression

Mammalian

Mutation

S957A

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA3 5' cMyc- SMCI-S966A

Plasmid

#32371

Depositor

Michael Kastan

Article

Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

Insert

SMC1A (SMC1A Human)

Tags

c-Myc

Expression

Mammalian

Mutation

S996A

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/NDUFS8/myc-His

Plasmid

#127489

Purpose

Expresses myc-tagged NDUFS8 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS8 (NDUFS8 Human)

Tags

myc-His

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-AC8(1-106)

Plasmid

#181958

Purpose

Residues 1-106 of rat adenylyl cyclase 8 (AC8) tagged with EGFP.

Depositor

Jin Zhang

Article

Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

Insert

EGFP-AC8(1-106) (Adcy8 Rat)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AMPKsub(TA)-YPet-NES

Plasmid

#84636

Purpose

Encodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

AMPKsub(TA)-YPet-NES

Tags

6xHis, Nuclear export signal (NES), T7 tag (gene …

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS DNAJB6b

Plasmid

#19555

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB6b

Tags

His

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP DNAJA4

Plasmid

#19494

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJA4 (DNAJA4 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Beta(4)Integrin-DeltaCYT

Plasmid

#16264

Depositor

Arthur Mercurio

Article

Clarke et al J Biol Chem. 1995 Sep 29. 270(39):22673-

Insert

Beta 4 Integrin (ITGB4 Human)

Expression

Mammalian

Mutation

truncated cytoplasmic tail introduced a stop cod…

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Jip1 JBD

Plasmid

#15751

Purpose

binds to Jnk

Depositor

Roger Davis

Article

Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

Insert

Jip1 (Jnk binding domain) (Mapk8ip1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

deleted 1-126 and 282-660

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only
AIRAP 10: PSMD14.TEV.GST.V1.pCDNA3

Plasmid

#21800

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

PSMD14 (Psmd14 Mouse)

Tags

GST and TEV

Expression

Mammalian

Available Since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA-TO-HA-SH3BP1 (RNAi-res)

Plasmid

#136645

Purpose

Expression of SH3BP1 in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC

Depositor

Maria Balda, Karl Matter

Article

Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

Insert

SH3BP1 (RNAi resistant) (SH3BP1 Human)

Tags

HA (MQDLPGNDNSTAG)

Expression

Mammalian

Mutation

GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTTC

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry GRK3 ct in pcDNA3.1

Plasmid

#64213

Purpose

mCherry fused between Cry2 and GRK3 ct to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 PHR (CRY2 Mustard Weed)
GRK3 ct (GRK3 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only