We narrowed to 22,191 results for: his
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Plasmid#222207PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with a C-terminal His tagDepositorInsertfghA
UseTags6x HisExpressionBacterialMutationPromoterT7Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB109
Plasmid#222208PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the K10N mutation and a C-terminal His tagDepositorInsertfghA (K10N)
UseTags6x HisExpressionBacterialMutationChanged Lys10 to AsnPromoterT7Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB110
Plasmid#222209PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G258D mutation and a C-terminal His tagDepositorInsertfghA (G258D)
UseTags6x HisExpressionBacterialMutationChanged Gly258 to AspartatePromoterT7Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
P15-pZE-SUMO-PiTTA
Plasmid#204630PurposeColE1 ori, KanR, TetR, Tet promoter with a codon optimized piTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.DepositorInsertHis-SUMO-TEV-BuTTA
UseSynthetic BiologyTagsHis Tag; SUMO Tag; TEV Protease SiteExpressionBacterialMutationPromoterPLtetO-1 promoterAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
P17-pZE-SUMO-CsTTA
Plasmid#204631PurposeColE1 ori, KanR, TetR, Tet promoter with a codon optimized csTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.DepositorInsertHis-SUMO-TEV-CsTTA
UseSynthetic BiologyTagsHis Tag; SUMO Tag; TEV Protease SiteExpressionBacterialMutationPromoterPLtetO-1 promoterAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
P18-pZE-SUMO-BuTTA
Plasmid#204632PurposeColE1 ori, KanR, TetR, Tet promoter with a codon optimized buTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.DepositorInsertHis-SUMO-TEV-BuTTA
UseSynthetic BiologyTagsHis Tag; SUMO Tag; TEV Protease SiteExpressionBacterialMutationPromoterPLtetO-1 promoterAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
P24-pZE-SUMO-KaTTA
Plasmid#204633PurposeColE1 ori, KanR, TetR, Tet promoter with a codon optimized kaTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.DepositorInsertHis-SUMO-TEV-KaTTA
UseSynthetic BiologyTagsHis Tag; SUMO Tag; TEV Protease SiteExpressionBacterialMutationPromoterPLtetO-1 promoterAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 KATNAL2 residues 1-98
Plasmid#180619PurposeBacterial expression for KATNAL2 , residues 1-98. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-28.DepositorInsertKATNAL2 (KATNAL2 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 1-98PromoterT7Available SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 RPS6KC1 MIT, residues 235-316
Plasmid#180622PurposeBacterial expression for RPS6KC1 MIT domain, residues 235-316. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-87.DepositorInsertRPS6KC1 (RPS6KC1 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 235-316PromoterT7Available SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT021-LAT_HASA-HaloTag-3x FLAG
Plasmid#202505PurposeExpress high ASA Lat mutantDepositorInsertLAT (LAT Human)
UseTagsHaloTag-3x FLAGExpressionMammalianMutationSeveral leucine subsitutions in transmembrane dom…PromoterCMVAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Chinchilla–insM
Plasmid#191226PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationremoved methionine at 122 in the protein sequencePromoterPH and p10Available SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sandgrouse–insD
Plasmid#191228PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationremoved aspartic acid at 121 in the protein seque…PromoterPH and p10Available SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCC_162 SpCas9 KES(1107-1109)GG
Plasmid#179526PurposeFor bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tagDepositorInsertSpCas9 KES(1107-1109)GG
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationreplaced KES (residues 1107-1109) with GGPromoterAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCC_163 SpCas9 K233A/K234A/K253A/K263A
Plasmid#179527PurposeFor bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tagDepositorInsertSpCas9 K233A/K234A/K253A/K263A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged lysines 233, 234, 253, and 263 to alaninePromoterAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_ANKRA2_ANK
Plasmid#178466PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertANKRA2_ANK (ANKRA2 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_KLC1_TPR
Plasmid#178477PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertKLC1_TPR (KLC1 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_IRF3_SMAD/FHA
Plasmid#178479PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertIRF3_IRF3 (IRF3 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLN492 (FuGW-G8p-antiPD1(HC)-1Pe)
Plasmid#105194PurposeModule 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab heavy chain transcripts containing miR1-Bs(Pe)DepositorInsertG8p-antiPD1(HC)-1Pe
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable SinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLN493 (FuGW-G8p-antiPD1(LC)-1Pe)
Plasmid#105195PurposeModule 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab light chain transcripts containing miR1-Bs(Pe)DepositorInsertG8p-antiPD1(LC)-1Pe
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable SinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-43
Plasmid#172759PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-43; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-43
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-35
Plasmid#172755PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-35; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-35
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-19
Plasmid#172748PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-19; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-19
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-3
Plasmid#172740PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-3; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-3
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-12
Plasmid#172744PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-12; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-12
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-14
Plasmid#172745PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-14; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-14
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM10 with rat Δ192Annexin A11
Plasmid#164498PurposeFor bacterial expression of rat His-tagged Δ192Annexin A11DepositorInsertrat Δ192Annexin A11 (Anxa11 Rat)
UseTagsHisExpressionBacterialMutationN-terminal truncationPromoterT7Available SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM10 with rat Δ188Annexin A11
Plasmid#164497PurposeFor bacterial expression of rat His-tagged Δ188Annexin A11DepositorInsertrat Δ188Annexin A11 (Anxa11 Rat)
UseTagsHisExpressionBacterialMutationN-terminal truncationPromoterT7Available SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMtb MCM
Plasmid#138296PurposeExpression of Mtb MCM small and large subunitDepositorInsertsmutA
mutB
UseTagsHis tag and TEV cleavage siteExpressionBacterialMutationinserted TEV cleavage site between His-tag and m…PromoterT7Available SinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEThT-MAHS delta MTP
Plasmid#90032PurposeTo express His-tagged MAHS in bacteriaDepositorInsertMAHS
UseTagsHisExpressionBacterialMutationDeletion of mitochondria targeting peptidePromoterT7Available SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGMEH-0X750-luxAB-DAS+4
Plasmid#49519PurposeHygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; replicates episomallyDepositorInsertP750-luxAB-DAS+4
UseTagsDAS+4 (AANDENYSENYADAS)ExpressionBacterialMutationPromoterP750Available SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21a-LIC
Plasmid#62303PurposeE. coli expression vector with N-terminal Hexa-HIS and TEV cleavageDepositorTypeEmpty backboneUseTags6xHisExpressionBacterialMutationPromoterT7Available SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQE81XN-mGold
Plasmid#218959PurposeExpresses Hisx6-Tagged mGold in bacteriaDepositorInsertmGold
UseTags6X-HisExpressionBacterialMutationPromoterT5Available SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE81XN-msYFP
Plasmid#218960PurposeExpresses Hisx6-Tagged msYFP in bacteriaDepositorInsertmsYFP
UseTags6X-HisExpressionBacterialMutationPromoterT5Available SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
883 pET15b PTEN
Plasmid#10936DepositorAvailable SinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLIC-SGC1
Plasmid#39187PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsHis TevExpressionBacterialMutationPromoterAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pJBL7065
Plasmid#167234Purposecell-free cascaded nitrate sensorDepositorInsertpNar-AKSIRV
UseSynthetic BiologyTags6X HisExpressionMutationPromoterAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7066
Plasmid#167235Purposecell-free cascaded mercury sensorDepositorInsertpMer-AKSIRV
UseSynthetic BiologyTags6X HisExpressionMutationPromoterAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
MZB133
Plasmid#228484Purpose"Empty" N-terminal TEV cleavable 6x His tag vector for rapid cloning/DNA Assembly via SmaI restriction site (Bacterial Expression)DepositorTypeEmpty backboneUseTags6xHis-TEVExpressionBacterialMutationPromoterT7 PromoterAvailable SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQE81XN-moxGFP2
Plasmid#218962PurposeExpresses Hisx6-Tagged moxGFP2 in bacteriaDepositorInsertmoxGFP2
UseTags6X-HisExpressionBacterialMutationPromoterT5Available SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only