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pFUW-TetO-NFIX

Plasmid

#207192

Purpose

doxycycline-inducible expression of Human NFIX in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-NFIX

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2Cre

Plasmid

#82415

Purpose

Lentiviral vector expressing Cre recombinase alongside Cas9 and an sgRNA cloning site

Depositor

David Feldser

Article

Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

Insert

Cre Recombinase

Use

CRISPR, Cre/Lox, and Lentiviral

Mutation

Mutated BsmBI cut site

Promoter

EFS (P2A)

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-ID3

Plasmid

#207215

Purpose

doxycycline-inducible expression of Human ID3 in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-ID3

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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A*11:01:01

Plasmid

#203281

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*11:01:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-KRAS4B(G12V)

Plasmid

#35633

Depositor

Daniel Haber

Article

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

K-Ras4B(G12V) (KRAS Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pT7C-HSP90AA1

Plasmid

#177658

Purpose

Recombinant protein synthesis of human HSP90AA1

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

HSP90AA1 (HSP90AA1 Human)

Tags

6xHis-Thrombin

Expression

Bacterial

Promoter

T7

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pT7C-HSPA8

Plasmid

#177661

Purpose

Recombinant protein synthesis of human HSPA8

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

HSPA8 (HSPA8 Human)

Tags

6xHis-Thrombin

Expression

Bacterial

Promoter

T7

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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B*57:03:01

Plasmid

#203260

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*57:03:01 (HLA-B Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-CREB3L2

Plasmid

#207207

Purpose

doxycycline-inducible expression of Human CREB3L2 in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-CREB3L2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-ER-KRAS(G12V)

Plasmid

#35635

Depositor

Daniel Haber

Article

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

K-Ras4B(G12V) (KRAS Human)

Use

Lentiviral

Tags

ER

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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HA-SOCS1

Plasmid

#174571

Purpose

Human HA-tagged SOCS1 expression (N-term. tag) (CMV prom.)

Depositor

Mathew Garnett

Article

Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

Insert

HA-SOCS1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-SRGAP2A-EGFP

Plasmid

#168501

Purpose

Expression of full length SRGAP2 with a C-termonal EGFP fusion

Depositor

Franck Polleux

Article

Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

Insert

SRGAP2A (SRGAP2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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Cas9-NAT

Plasmid

#64329

Purpose

Cas9 expression cassette carried by a yeast single-copy episome plasmid

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette

Use

CRISPR

Expression

Yeast

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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B*35:03:01

Plasmid

#203255

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*35:03:01 (HLA-B Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xMx PylT_FLAG-Mx PylRS

Plasmid

#140011

Purpose

Plasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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AICSDP-55:DMD-mEGFP

Plasmid

#159744

Purpose

Homology arms and linker-mEGFP sequence for C-terminus tagging of human DMD via Cas9-excisable CAGGS-mCherry selection cassette

Depositor

Allen Institute for Cell Science

Insert

DMD Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (DMD Human)

Use

CRISPR; Donor template

Tags

linker-mEGFP

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-NFATC2

Plasmid

#207191

Purpose

doxycycline-inducible expression of Human NFATC2 in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-NFATC2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-ZEB2

Plasmid

#207203

Purpose

doxycycline-inducible expression of Human ZEB in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-ZEB2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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p7SL-neo-TET

Plasmid

#51285

Purpose

expresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassette

Depositor

Astrid Roy-Engel

Article

Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

Insert

7SL RNA (RN7SL2 Human)

Tags

neoTET cassette with a tetrahymena self-splicing …

Expression

Mammalian

Promoter

7SL upstream pollII enhancer

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-Mef2c

Plasmid

#207221

Purpose

doxycycline-inducible expression of Mouse Mef2c in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-Mef2c

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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