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pGEX-5X1 Ash2L full

Plasmid

#15549

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

Ash2L (ASH2L Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
GAL FLAG25QDProGFP p306

Plasmid

#15831

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q Delta Pro (HTT Human)

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only
GAL FLAG103QDProGFP p306

Plasmid

#15834

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 103Q Delta Pro (HTT Human)

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only
pSLQ10871

Plasmid

#183962

Purpose

U6-SKSKSO(crRNA array)-CAG-dCas12a(WT)-miniVPR

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Inserts

dCas12a(wildtype)
poly-crRNA array targeting Sox2-Klf4-Oct4

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D832A

Promoter

CAG and U6

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET151-GyrB

Plasmid

#216233

Purpose

S. aureus USA300 GyrB Codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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PITPbeta-GFP

Plasmid

#58274

Purpose

mammalian expression of GFP-PITP beta

Depositor

Shamshad Cockcroft

Article

Morgan et al Biochem J. 2006 Sep 15;398(3):411-21.

Insert

PITP beta (PITPNB Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
FRB-BFP-GCN4-ppKin14

Plasmid

#240426

Purpose

-end oriented kinesin for rapalog induced pulling experiments

Depositor

Anna Akhmanova

Article

Chen et al Elife. 2022 Jul 5;11:e77892. doi: 10.755

Insert

ppKin14Vib

Tags

FRB, GCN4, and tagBFP

Expression

Mammalian

Mutation

deleted amino acids 1-850

Available Since

July 30, 2025

Availability

Academic Institutions and Nonprofits only

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pRevTRE-hygro-GFP-Crumbs3a WT

Plasmid

#195217

Purpose

Retroviral Tet response vector containing GFP tagged Crumbs3a

Depositor

David Bryant, Ben Margolis

Article

Schluter et al Mol Biol Cell. 2009 Nov;20(22):4652-63.

Insert

CRB3A (CRB3 Human)

Use

Retroviral

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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VinTS-tCRMod-GGSGGS7

Plasmid

#111764

Purpose

Expresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 module

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-BiP-Myc-KDEL P495L

Plasmid

#27166

Depositor

Ron Prywes

Article

Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

Insert

BiP (HSPA5 Human)

Tags

KDEL and Myc

Expression

Mammalian

Mutation

Peptide Binding Mutant: P495L

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
pEND-446_Cacnes_ProA

Plasmid

#225609

Purpose

Suicide C. acnes vector to generate ProA (PPA_RS04250) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-BiP-Myc-KDEL T37G

Plasmid

#27165

Depositor

Ron Prywes

Article

Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

Insert

BiP (HSPA5 Human)

Tags

KDEL and Myc

Expression

Mammalian

Mutation

ATP bound form: T37G

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
pCS2+ Gal4-GBP2-IRES-GBP7-p65

Plasmid

#50020

Purpose

Multi-purpose expression of a GFP-dependent transcription factor

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

Gal4DBD-GBP2-IRES-NLS-GBP7-p65AD

Use

Multi-purpose expression

Promoter

CMV-immediate early promoter and SP6 promoter

Available Since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pUCmEos3.2:cat

Plasmid

#78100

Purpose

Plasmid template that encodes the photoconvertible fluorescent protein mEos3.2 next to chloramphenicol resistance gene (cat). This template was used to copy out mEos3.2-cat for genetic tagging.

Depositor

Peng Chen

Article

Chen et al Nat Commun. 2015 Jul 6;6:7445. doi: 10.1

Insert

mEos3.2-cat

Expression

Bacterial

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only
pGDP4 ermC

Plasmid

#112884

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

ermC (ermM Staphylococcus epidermis)

Tags

6xHis

Expression

Bacterial

Promoter

Plac

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-tetO-CTR1-shRNA

Plasmid

#53180

Purpose

Expresses shRNA against human CTR1 from puromycin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

CTR1 (SLC31A1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only
MAC3-EGFR

Plasmid

#222610

Purpose

Expression of MAC3-tagged EGFR

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFR (EGFR Human)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pMB1-A-TelR

Plasmid

#204979

Purpose

Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Added replications elements for Acinetobacter spe…

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pGDP3 cat

Plasmid

#112888

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

cat (Brevibacillus brevis) (cat Brevibacillus brevis)

Tags

6xHis

Expression

Bacterial

Promoter

Pbla

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pmKate2-MS2coatProtein

Plasmid

#103833

Purpose

Live cell reader for MS2 loop RNA production

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

mKate2-MS2 coat protein (cp )

Expression

Mammalian

Mutation

MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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