We narrowed to 12,677 results for: sequence

1. pAAV-CAGGS-Flex-GFP

   Plasmid

   #197883

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   CAG

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGZ110 (pFA6a-3FLAG-MNase-TRP1)

   Plasmid

   #70233

   Purpose

   ChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA-Trp1 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope tag

   Depositor

   Steven Henikoff

   Article

   Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

   Insert

   3xFLAG tag and aa 83-231 of Mnase
   

   Use

   Yeast genomic targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTYB3-human PUM1-HD MUT6-2 7-2 (N1043S-Q1047E-S1079N-E1083Q)-intein-CBD

   Plasmid

   #73300

   Purpose

   Expresses mutant human PUM1-HD

   Depositor

   Traci Hall

   Article

   Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

   Insert

   human PUM1 Pumilio homology domain (PUM1 Human)
   

   Use

   Tags

   intein

   Expression

   Bacterial

   Mutation

   N1043S-Q1047E-S1079N-E1083Q

   Promoter

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

4. NELFE-mOrange pHis-//

   Plasmid

   #53303

   Purpose

   Expresses His-tagged NELF-E-mOrange fusion protein.

   Depositor

   John Lis

   Article

   Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

   Insert

   NELF-E protein (Nelf-E Fly)
   

   Use

   Tags

   His and mOrange

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDblet

   Plasmid

   #8848

   Depositor

   Joel Huberman

   Article

   Brun et al Gene. 1995 Oct 16. 164(1):173-7.

   Insert

   ARS doublet
   

   Use

   Yeast cloning vector

   Tags

   Expression

   Yeast

   Mutation

   This vector contains, between AatII sites, a dire…

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAV-U6+27-Linear-2_PolyU(1)_Tornado-Corn

   Plasmid

   #159487

   Purpose

   Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-2_PolyU(1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFX-Venus-Sec61β

   Plasmid

   #174467

   Purpose

   Express Venus with endoplasmic reticulum-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   Venus with endoplasmic reticulum-targeting sequence
   

   Use

   Tags

   Sec61 subunit beta

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-EF1a-MTS-FLAG-LOV*

   Plasmid

   #202208

   Purpose

   Plasmid for the generation of a lentiviral vector encoding the photoproximity labeling catalyst LOV* fused to the mitochondria targeting sequence (MTS) in mammalian cells

   Depositor

   Thomas Muir

   Article

   Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

   Insert

   Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, aa 1-24
   

   Use

   Lentiviral

   Tags

   FLAG and LOV*

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFX-H2B-EGFP

   Plasmid

   #175839

   Purpose

   Express EGFP with nucleus-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   EGFP with nucleus-targeting sequence
   

   Use

   Tags

   H2B (human histone 2B)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICSL80036

    Plasmid

    #68259

    Purpose

    Level 0 Golden Gate Part: Coding sequence, hygromycin phophotransferase II (Escherichia coli)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Coding sequence, hygromycin phophotransferase II (Escherichia coli)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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