159,333 results

1. pLenti_CMV: Flag-Akaluc_SV40:PuroR

   Plasmid

   #183048

   Purpose

   Expresses FLAG-tagged AkaLuc (Far-Red Firefly luciferase) in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

   Insert

   AkaLuc
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO_CalR_HaloTag9_KDEL

   Plasmid

   #175540

   Purpose

   Expression of HaloTag9 in the endoplasmid reticulum of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   CalR-HaloTag9-KDEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_IDH1_p.R132H

   Plasmid

   #81686

   Purpose

   Gateway Donor vector containing IDH1, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   IDH1 (IDH1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   R132H

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. HyPer7.2DAAO-NLS

   Plasmid

   #168302

   Purpose

   Chemogenetic fluorescent H2O2 reporter targeted to the nucleus

   Depositor

   Thomas Michel

   Article

   Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

   Insert

   HyPer7.2DAAO-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 236HSC WT (BRCA2)

   Plasmid

   #16246

   Depositor

   Mien-Chie Hung

   Article

   Wang et al Cancer Res. 2002 Mar 1. 62(5):1311-4.

   Insert

   BRCA2 (BRCA2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. APEX2-csGBP

   Plasmid

   #108874

   Purpose

   Expression of APEX2 fused to a conditionally stable nanobody against GFP

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   APEX2-csGBP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBABE-neo-hTERT

   Plasmid

   #1774

   Purpose

   Retroviral expression of hTERT. Used to create immortalized cell lines.

   Depositor

   Bob Weinberg

   Article

   Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

   Insert

   hTERT (TERT Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. pL-CRISPR.EFS.tRFP

   Plasmid

   #57819

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-tRFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMOD_B2103

   Plasmid

   #91061

   Purpose

   Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CmYLCV

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-4T MDM2 WT

    Plasmid

    #16237

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pN1-PinkyCaMP

    Plasmid

    #232861

    Purpose

    A vector expressing the mScarlet based calcium indicator PinkyCaMP under CMV promoter

    Depositor

    Olivia Masseck

    Article

    Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

    Insert

    PinkyCaMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

    Plasmid

    #179105

    Purpose

    pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. rEstus

    Plasmid

    #209767

    Purpose

    To express a bright and sensitive genetically encoded voltage indicator in mammalian cells.

    Depositor

    Stefan Heinemann

    Article

    Rühl et al bioRxiv 2023.

    Insert

    rEstus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRDA_816

    Plasmid

    #201157

    Purpose

    EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRa

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    5x-ALFA tag-dEnAsCas12a-VP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    D908A

    Promoter

    EFS

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG425GPD-ccdB

    Plasmid

    #14154

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. AbVec1.1-IGLC2-XhoI

    Plasmid

    #99575

    Purpose

    Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCVD442

    Plasmid

    #11074

    Depositor

    Michael Donnenberg, James Kaper

    Article

    Donnenberg et al Infect Immun. 1991 Dec . 59(12):4310-7.

    Insert

    sacB (sacB Bacillus subtilis)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEC3135 (pERASE)

    Plasmid

    #220306

    Purpose

    Enables scarless gene deletions in Streptococcus pyogenes using the counterselection marker pheS*

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-C1-LgBiT-MYPT1(1-314)(KAKA)-Flag

    Plasmid

    #224894

    Purpose

    Mammalian expression of LgBiT-MYPT1(1--314)

    Depositor

    Mathieu Bollen

    Article

    Claes et al Cell Chem Biol. 2023 Dec 21;30(12):1666-

    Insert

    LgBiT-MYPT1(1-314)KAKA-Flag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-puro

    Plasmid

    #39481

    Purpose

    Tetracycline inducible 3rd generation lentiviral vector

    Depositor

    Ie-Ming Shih

    Article

    Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5 and 6XHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge