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We narrowed to 9,302 results for: BLI;

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Showing: 8201 - 8250 of 9302 results

  2. pCAGGS-Twin-strep-TEV-DGKδ2-DSAMD

    Plasmid
    #223721
    Purpose
    Express human diacylglycerol kinase delta 2- SAM domain deletion mutant (N-terminal TEV protease cleavable Twin-Strep-fusion protein) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    DGKD (DGKD Human)
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted sterile alpha motif domain (deleted amino…
    Promoter
    CAG and chicken β-actin promoter
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    223721-plasmid-map-sequence-id-457432

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  3. pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-Emerald

    Plasmid
    #214468
    Purpose
    Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    ZFYVE27 (ZFYVE27 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    S14D S25D S32D T261D
    Available Since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    214468-plasmid-map-sequence-id-424065

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  4. pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-mCherry

    Plasmid
    #214471
    Purpose
    Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    ZFYVE27 (ZFYVE27 Human)
    mCherry
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    S14D S25D S32D T261D
    Available Since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214471-plasmid-map-sequence-id-425103

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  8. Venus-Bik-del72-136-pEGFP-C3

    Plasmid
    #191411
    Purpose
    Transfection of this plasmid will express "VBik-d72-136" (Venus fused to the N-terminus of the BH3-only protein, Bik that has amino acids 72-136 deleted).
    Depositor
    David Andrews
    Insert
    Bcl-2-interacting killer (BIK Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    with deletion of amino acids 72-136 (del72-136) i…
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    191411-plasmid-map-sequence-id-376884

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  9. pcDNA3_HLA-cmyc-GRM3 C127A TMD Y616M

    Plasmid
    #140454
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and Y616M mutant
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, tyrosine 616 to methioni…
    Available Since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140454-plasmid-map-sequence-id-292302

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  10. pcDNA3_HLA-cmyc-GRM3 C127A TMD L624N

    Plasmid
    #140455
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and L624N mutant
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, leucine 624 to asparagine
    Available Since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140455-plasmid-map-sequence-id-292306

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  11. pcDNA3_HLA-cmyc-GRM3 C127A TMD A634N

    Plasmid
    #140456
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and A634N mutant
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, alanine 634 to asparagine
    Available Since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140456-plasmid-map-sequence-id-292300

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  12. pCEP4_HLA-flag-GRM3 C127A-VN

    Plasmid
    #140449
    Purpose
    Mammalian expression plasmid for FLAG-tagged mGluR3 fused to the N-terminal half of split fluorescent Venus (VN)
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Tags
    FLAG tag, Signal/leader sequence from HLA class I…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine
    Available Since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140449-plasmid-map-sequence-id-292293

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  13. pCEP4_HLA-myc-GRM3 C127A-VC

    Plasmid
    #140450
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 fused to the C-terminal half of split fluorescent Venus (VC)
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine
    Available Since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140450-plasmid-map-sequence-id-292295

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  14. pcDNA3_HLA-cmyc-GRM3 C127A TMD I578A

    Plasmid
    #140452
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and I578A mutant
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, isoluecine 578 to alanine
    Available Since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140452-plasmid-map-sequence-id-292301

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  15. pcDNA3_HLA-cmyc-GRM3 C127A TMD F588V

    Plasmid
    #140453
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and F588V mutant
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, phenylalanine 588 to val…
    Available Since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140453-plasmid-map-sequence-id-292308

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  17. pB6-Id1-IRES-loxP-NeoR-loxP-DTA-TK

    Plasmid
    #158616
    Purpose
    B6J Id1-LSL-DTA allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Id1 (Id1 Mouse)
    Use
    Cre/Lox and Mouse Targeting
    Expression
    Mammalian
    Mutation
    Insertion of IRES-loxP-NeoR-loxP-DTA after the st…
    Available Since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    158616-plasmid-map-sequence-id-333472

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  19. pTEF1-UDH

    Plasmid
    #166679
    Purpose
    Yeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).
    Depositor
    Claudia Vickers
    Article
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Insert
    UDH
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    TEF1
    Available Since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    166679-plasmid-map-sequence-id-329863

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  20. wtVP1 only

    Plasmid
    #166673
    Purpose
    Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1, with GAL1 promoter. Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Insert
    Wild-type Murine polyomavirus VP1
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    GAL1
    Available Since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    166673-plasmid-map-sequence-id-329825

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  21. psiCHECK2 3'UTR KGA

    Plasmid
    #110402
    Purpose
    Luminescence-based glutaminase KGA isoform 3'UTR-mediated RNA stability reporter
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110402-plasmid-map-sequence-id-213697

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  22. pX330.puro sgRNA KGA Stop1

    Plasmid
    #110405
    Purpose
    CRISPR/Cas9 vector with sgRNA for glutaminase KGA isoform C-Terminal Knock-in and puromycin resistance
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110405-plasmid-map-sequence-id-213578

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  24. p1A CbbL-

    Plasmid
    #162693
    Purpose
    p1A negative control expressing inactive CbbLS (carbamylated K194M mutation) and Prk
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    H. neapolitanus CbbLS and S. elongatus Prk
    Tags
    N terminal 6x His tag on PRK
    Expression
    Bacterial
    Mutation
    CbbL K194M (inactive rubisco)
    Promoter
    PLtet0-1 promoter
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162693-plasmid-map-sequence-id-324169

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  30. pET14b-dabAB2 D353A

    Plasmid
    #133016
    Purpose
    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag
    Depositor
    David Savage
    Article
    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038
    Inserts
    dabB2
    dabA2
    Tags
    6xHis, StrepII tag, and sfGFP
    Expression
    Bacterial
    Mutation
    D353A
    Promoter
    t7
    Available Since
    Nov. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    133016-plasmid-map-sequence-id-262984

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  31. pET14b-dabAB2 C351A

    Plasmid
    #133014
    Purpose
    pET14b carrying the dabA2 gene (Uniprot: D0KWS7)  with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag
    Depositor
    David Savage
    Article
    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038
    Inserts
    dabB2
    dabA2
    Tags
    6xHis, StrepII tag, and sfGFP
    Expression
    Bacterial
    Mutation
    C351A
    Promoter
    t7
    Available Since
    Nov. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    133014-plasmid-map-sequence-id-262980

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  32. pTH862-CEN-slowmaxRLuc/minCFLuc

    Plasmid
    #115371
    Purpose
    Expresses translation initiation-controlled Renilla luciferase and translation elongation-controlled firefly luciferase
    Depositor
    Tobias von der Haar
    Inserts
    Firefly Luciferase (codon minimised)
    Gcn4 5'-UTR + Renilla luciferase (codon optimised)
    Expression
    Yeast
    Mutation
    All codons have been changed for the most favoura…
    Promoter
    ADH1 and TDH3
    Available Since
    June 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    115371-plasmid-map-sequence-id-247306

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  34. pGS1613

    Plasmid
    #63789
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal tDimer2 Strep-tag II followed by HIS5 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    tDimer2-Strep-tag II followed by HIS5
    Use
    E. coli cloning vector
    Tags
    tDimer2-Strep-tag II
    Promoter
    none
    Available Since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    63789-plasmid-map-sequence-id-110438

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  35. pGS1122

    Plasmid
    #63790
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Twin-Strep tag followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eSapphire-Twin-Strep tag followed by URA3
    Use
    E. coli cloning vector
    Tags
    eSapphire-Twin-Strep tag
    Promoter
    none
    Available Since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63790-plasmid-map-sequence-id-106477

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  36. pGS1412

    Plasmid
    #63787
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Strep-tag II followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eCitrine-Strep-tag II followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    eCitrine-Strep-tag II
    Promoter
    none
    Available Since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63787-plasmid-map-sequence-id-106472

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  50. PB-TO-oNGN2

    Plasmid
    #198397
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Inserts
    NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    rtTA3G
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…
    Promoter
    CAG, EF1a, and TRE3G
    Available Since
    April 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    198397-plasmid-map-sequence-id-389535

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Showing: 8201 - 8250 of 9302 results