We narrowed to 9,648 results for: ADA

1. pYPQ121-mTCABE-T

   Plasmid

   #218173

   Purpose

   Express plastid monomeric TALE-linked CABE-T, composed of TALE, a catalytically impaired, full length DddA variant (E1347A), a TadA-derived deaminase, and UGI

   Depositor

   Yiping Qi

   Article

   Wang et al Plant Biotechnol J. 2024 May 6. doi: 10.

   Insert

   TALE-Intron-TadAC-DddA full (E1347A)-UGI
   

   Use

   Plastid base editing

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-FKBP-GFP-OPTN

   Plasmid

   #208866

   Purpose

   Stably express GFP-tagged OPTN for CID induced mitophagy

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   OPTN (OPTN Human)
   

   Use

   Tags

   FKBP-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. RT-5

   Plasmid

   #220442

   Purpose

   Construct used to generate the PUF3/PUP2/3HA tagging strains. (aka pBSII/PUF3 5’ flank/PUP2-DADA/3HA/URA3/PUF3 3’ flank)

   Depositor

   Marvin Wickens

   Article

   Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

   Insert

   PUF3 (puf-3 Nematode)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C1-IFT56

   Plasmid

   #218727

   Purpose

   Expresses N-terminally EGFP-tagged IFT56 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Nozaki et al J Cell Sci. 2016 Dec 7. pii: jcs.197004.

   Insert

   IFT56 (TTC26 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3xMS2 TR knockin sgRNA 1

   Plasmid

   #207607

   Purpose

   sgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   cgactcgcccggcagcgcac
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3xMS2 TR knockin sgRNA 2

   Plasmid

   #207608

   Purpose

   sgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   acccccaaacctgactgact
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. TR knockout HRD

   Plasmid

   #207579

   Purpose

   Homologous recombination donor replacing TR with a puro cassette.

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   puro resistance cassette flanked by TR locus sequences excluding TR
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-Duet_OPTN-thrombin-GST

   Plasmid

   #216843

   Purpose

   For expression in E. coli of OPTN (Internal ref. SMC1789)

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   Optineurin (OPTN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTcI39-PRM-wasabi

   Plasmid

   #195555

   Purpose

   Temperature-dependent expression of mWasabi reporter with transcription activation by TcI39

   Depositor

   Mikhail Shapiro

   Article

   Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

   Inserts

   mWasabi
   TcI39
   

   Use

   Tags

   6x His and AANDENYADAS degradation tag

   Expression

   Bacterial

   Mutation

   A67T

   Promoter

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. ipGL2Cas

    Plasmid

    #210555

    Purpose

    Binary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by GL2 promoter.

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    GL2p::XVE::UBQ10t::LexA::SpCas9::PEA3AT::VenusSSM
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. ipYUC2Cas-LT1

    Plasmid

    #210559

    Purpose

    Binary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by M. polymorpha YUC2 promoter.

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pMpEF1::XVE::UBQ10t::LexA::SpCas9::PEA3AT::pU6::AAVS1gRNA::pMpEF1::LT1::Hygromycin
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF1Cas-LT1

    Plasmid

    #210556

    Purpose

    Binary vector expressing the Lineage Tracing system. Cas9 expression driven by M. polymorpha EF1 promoter. LT1 reporter under control of MpEF1p

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pMpEF1::Cas9::PEA3AT::U6p::AAVS1gRNA::EF1p::LT1::Hygromycin
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGGC-LexACasPea

    Plasmid

    #210539

    Purpose

    Cloning estradiol-inducible expression cassette containi LexA XVE binding sequence followed by SpCas9 CDS and PEA3AT as GreenGate module C

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    LexA::SpCas9::PEA3AT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGGA-pCLV3

    Plasmid

    #210528

    Purpose

    Cloning A. thaliana CLV3 promoter as GreenGate module A

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pCLV3 (CLV3 Mustard Weed)
    

    Use

    Synthetic Biology; Golden gate compatible cloning…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMx/ATP10D-HA

    Plasmid

    #209234

    Purpose

    Mammalian expression of ATP10D

    Depositor

    Hye-Won Shin

    Article

    Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

    Insert

    ATP10D (ATP10D Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-WDR5-P2A-SH2-P2A-Ras-CAAX

    Plasmid

    #209712

    Purpose

    Mammailan expression of WDR5, SH2, and HRas separated by P2A.

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    WDR5-P2A-SH2-P2A-Ras-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-NLS-MBP-rATOM(WDR5)

    Plasmid

    #209710

    Purpose

    Mammalian expression of r-ATOM biosensor for WDR5 with nuclear localization signal

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    NLS-MBP-rATOM(WDR5)
    

    Use

    Tags

    NLS-MBP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-hARFGAP3-HA

    Plasmid

    #206411

    Purpose

    Mammalian expression of hARFGAP3

    Depositor

    Kazuhisa Nakayama, Hye-Won Shin

    Article

    Saitoh et al J Biol Chem. 2009 May 15;284(20):13948-1

    Insert

    ARFGAP3 (ARFGAP3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHWBF09exp

    Plasmid

    #130833

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

    Insert

    MLKP1
    

    Use

    Tags

    eGFP-FLAG-HA

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    Sept. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. MLKP2del4

    Plasmid

    #130837

    Purpose

    Expression of plant LINC fluorescent fusion protein for cell biology

    Depositor

    Hank Bass

    Article

    Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

    Insert

    MLKP2
    

    Use

    Tags

    eGFP-FLAG-HA

    Expression

    Plant

    Mutation

    Deletion of terminal four amino acid residues

    Promoter

    CaMV 35S

    Available since

    Sept. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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