162,619 results

1. pGEX-DAXX-D/E-His

   Plasmid

   #179619

   Purpose

   Express DAXX D/E region in bacteria

   Depositor

   Xiaolu Yang

   Article

   Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

   Insert

   death domain associated protein (DAXX Human)
   

   Use

   Tags

   GST, His, and TEV

   Expression

   Bacterial

   Mutation

   amino acids 414-505 of DAXX

   Promoter

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJUMP29-1D(sfGFP)

   Plasmid

   #126963

   Purpose

   Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJUMP29-1C(sfGFP)

   Plasmid

   #126962

   Purpose

   Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJUMP29-1B(sfGFP)

   Plasmid

   #126961

   Purpose

   Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EC2_1_dCas9_sgRNA

   Plasmid

   #163710

   Purpose

   Yeast low copy plasmid with dCas9 and sgRNA expression cassette

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-miSFIT-2x

   Plasmid

   #124691

   Purpose

   Tuning expression of EGFP

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

   Plasmid

   #61364

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…

   Promoter

   CMV

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJUMP29-1E(sfGFP)

   Plasmid

   #126982

   Purpose

   Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCheY Tc delta C

   Plasmid

   #13520

   Depositor

   James McKerrow

   Article

   Eakin et al J Biol Chem. 1993 Mar 25. 268(9):6115-8.

   Insert

   cruzain
   

   Use

   Tags

   CheY

   Expression

   Bacterial

   Mutation

   Truncated variant: stop codon after Gly212

   Promoter

   Available since

   March 11, 2009

   Availability

   Academic Institutions and Nonprofits only

10. XP7 Sonic hh

    Plasmid

    #16983

    Depositor

    Randall Moon

    Insert

    sonic hedgehog
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only