We narrowed to 12,476 results for: pcdna

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pcDNA3-FLAG-RIPK3 K50A

Plasmid

#78817

Purpose

To overexpress RIPK3 K50A in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

K50A

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3−3xFLAG−AGO2(S387A)

Plasmid

#220462

Purpose

Express S387 phospho-null mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Human AGO2 (AGO2 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Serine 387 to alanine

Promoter

CMV

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3−3xFLAG−AGO2(S387D)

Plasmid

#220463

Purpose

Express S387 phosphomimetic mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Human AGO2 (AGO2 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Serine 387 to aspartate

Promoter

CMV

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Tau Authentic Alu 10-12V337M

Plasmid

#194169

Purpose

Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau V337M mutant. Expresses tau circular RNA 12-->10 VM with 3X flag tag in exon 10.

Depositor

Stefan Stamm

Article

Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

Insert

Microtubule-associated protein tau 10-12 V337M

Tags

3X flag

Expression

Mammalian

Mutation

Changed Valine 337 to Methinonine

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-f:PGC1(delta CTD)

Plasmid

#1030

Depositor

Bruce Spiegelman

Article

Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

Insert

PGC1a (Ppargc1a Mouse)

Tags

Flag

Expression

Mammalian

Mutation

delta CTD

Available Since

Oct. 4, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-214

Plasmid

#26313

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-214 (MIR214 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-Flag-H2A K13R

Plasmid

#63566

Purpose

Expression of human H2A with K13R tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine at 13 is now Arginine

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-Flag-H2A K15R

Plasmid

#63567

Purpose

Expression of human H2A with K15R tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine at 15 is now Arginine

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-RIPK3[S227D]-FLAG

Plasmid

#199320

Purpose

mutated phosphorylation site (MLKL interaction permanent active)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 227 to Aspartic Acid

Available Since

May 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S227A]-FLAG

Plasmid

#199318

Purpose

mutated phosphorylation site (MLKL interaction inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 227 to Alanine

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-YOD1 C160S

Plasmid

#49785

Purpose

Express YOD1 C160S mutant with a N-terminal Flag-tag in mammalian cells

Depositor

Christian Schlieker

Article

Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

Insert

YOD1 (YOD1 Human)

Tags

Flag-tag

Mutation

C160S

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3puro-mGFP-ROCK2-N (1-941)

Plasmid

#225473

Purpose

Express mEGFP-fusion protein of ROCK2 fragment (1-941)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

ROCK2 (ROCK2 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

N terminal fragment (1-941)

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-NBid-PhoCl1-CBid

Plasmid

#164050

Purpose

Optogenetic manipulation of cell apoptosis using PhoCl1

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NBid-PhoCl1-CBid

Tags

kozak sequence

Expression

Mammalian

Promoter

CMV promotor

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FRB-LplA(W37V)

Plasmid

#35608

Depositor

Alice Ting

Article

Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

Insert

FRB-LplA(W37V)

Tags

FLAG (internal fusion), HA, and His Tag

Expression

Mammalian

Mutation

LplA has Trp37 to Val mutation

Promoter

CMV

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS HSPA2

Plasmid

#19539

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 2 (HSPA2 Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-142

Plasmid

#26309

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-142 (MIR142 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 -5'cMyc-SMCl- S957A

Plasmid

#32368

Depositor

Michael Kastan

Article

Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

Insert

SMC1A (SMC1A Human)

Tags

c-Myc

Expression

Mammalian

Mutation

S957A

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(-)-C2C1-ErNaR-TS-EYFP-ES

Plasmid

#209851

Purpose

Expression of human codon-optimized light-driven sodium pump ErNaR in mammalian cells

Depositor

Volker Busskamp

Article

Podoliak et al Nat Commun. 2024 Apr 10;15(1):3119. doi:

Insert

light-driven sodium pump ErNaR

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TAT-2-130-FLAG

Plasmid

#35984

Depositor

Susana Valente

Article

Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

Insert

transactivating protein (tat )

Tags

Flag

Expression

Mammalian

Mutation

tat from HIV2 130 amino acids

Available Since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HCMVgL-FLAG (Merlin)

Plasmid

#136450

Purpose

Mammalian expression plasmid for HCMV glycoprotein L (Merlin strain) with FLAG tag

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein L (UL115 Human betaherpesvirus 5)

Tags

Short SGGSG linker and FLAG tag

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3 5' cMyc- SMCI-S966A

Plasmid

#32371

Depositor

Michael Kastan

Article

Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

Insert

SMC1A (SMC1A Human)

Tags

c-Myc

Expression

Mammalian

Mutation

S996A

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-HCMVgO-sfGFP (Merlin)

Plasmid

#136452

Purpose

Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) fused to superfolder GFP

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein O (UL74 Human betaherpesvirus 5)

Tags

Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP DNAJA4

Plasmid

#19494

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJA4 (DNAJA4 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Beta(4)Integrin-DeltaCYT

Plasmid

#16264

Depositor

Arthur Mercurio

Article

Clarke et al J Biol Chem. 1995 Sep 29. 270(39):22673-

Insert

Beta 4 Integrin (ITGB4 Human)

Expression

Mammalian

Mutation

truncated cytoplasmic tail introduced a stop cod…

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3_DRD1-NTEV-TCS-GV-2xHA

Plasmid

#194358

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

DRD1-NTEV-TCS-GV-2xHA (DRD1 Synthetic, Human)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-MARS-p85(iSH2)

Plasmid

#205236

Purpose

Expresses PLEKAH5 aa 143-271 (K163A and R164A) fused to the iSH2 domain of mouse p85beta

Depositor

Jeremy Baskin

Article

Cao et al Nat Cell Biol. 2024 Oct;26(10):1804-1816

Inserts

PLEKHA5(143-271)-K163A/R164A (PLEKHA5 Human)
p85(iSH2) (Pik3r1 Mouse)

Tags

Nuclear Export Sequence and mCherry

Expression

Mammalian

Mutation

amino acids 143-271 of PLEKHA5 (GenBank reference…

Promoter

CMV

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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AIRAP 10: PSMD14.TEV.GST.V1.pCDNA3

Plasmid

#21800

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

PSMD14 (Psmd14 Mouse)

Tags

GST and TEV

Expression

Mammalian

Available Since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jip1 JBD

Plasmid

#15751

Purpose

binds to Jnk

Depositor

Roger Davis

Article

Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

Insert

Jip1 (Jnk binding domain) (Mapk8ip1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

deleted 1-126 and 282-660

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-137

Plasmid

#26327

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-137 (Mir137 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO HIS DNAJB6b

Plasmid

#19555

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB6b

Tags

His

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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