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pCIG3 (pCMV-IRES-GFP version 3)

Plasmid

#78264

Purpose

Lentiviral empty cloning vector containing IRES and GFP

Depositor

Felicia Goodrum

Article

Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CMV, lentiviral LTR

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgLKB1 clone2

Plasmid

#162126

Purpose

Lentiviral sgRNA plasmid targeting human LKB1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgLKB1 (STK11 Human)

Use

Lentiviral

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG_hPPP1R15B_4-713_mCherry_UK1298

Plasmid

#80707

Purpose

mammalian expression N-term FLAG and C-term mCherry hPPP1R15B (CReP 4-713)

Depositor

David Ron

Article

Chen et al Elife. 2015 Mar 16;4. doi: 10.7554/eLife

Insert

human PPP1R15B (PPP1R15B Human)

Tags

mCherry

Expression

Mammalian

Mutation

CDS 4-713 no stop codon

Promoter

CMV

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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FUGW-ER-TurboID

Plasmid

#200965

Purpose

3rd gen lentiviral plasmid for expressing endoplasmic reticulum localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

ER-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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p1A

Plasmid

#162692

Purpose

Expresses H. neapolitanus CbbLS and S. elongatus Prk. Rescues CCMB1 in M9 glycerol at 10% CO2

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

H. neapolitanus CbbLS and S. elongatus Prk

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFE-Reu-FI-prk

Plasmid

#162696

Purpose

Expresses R. eutropha Form IC rubisco and S. elongatus Prk

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

R. eutropha CbbLS and S. elongatus Prk

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Mito-TurboID

Plasmid

#200960

Purpose

Transiently expresses mitochondria localized TurboID with V5 tags

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

mito-TurboID

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pFE-Rs-FI-prk

Plasmid

#162697

Purpose

Expresses R. spaeroides Form IC rubisco and S. elongatus Prk

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

R. spaeroides Form IC rubisco and S. elongatus Prk

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET3a-OSF-CypA

Plasmid

#79039

Purpose

Bacterial vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OneSTrEP-FLAG- Cyclophilin A (PPIA Human)

Tags

OneSTrEP-FLAG

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-HaloTag7

Plasmid

#167266

Purpose

Overexpression of HaloTag7 in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

HaloTag7

Tags

His x10

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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PB_iMYOD1_P2A_GFP_Puro

Plasmid

#168804

Purpose

PiggyBac transposon construct with doxycycline inducible MYOD1 + GFP expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

MYOD1 (MYOD1 Human, Synthetic)

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGIGYF-WFFtoAAA_AH

Plasmid

#148939

Purpose

Insect Expression of DmGIGYF-WFFtoAAA

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF-WFFtoAAA (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4EHP-W85A_AH

Plasmid

#148917

Purpose

Insect Expression of Dm4EHP-W85A

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

Dm4EHP-W85A (4EHP Fly)

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-DIO-PSD95.FingR-eGFP-CCR5TC

Plasmid

#126216

Purpose

Labeling of excitatory synapses (green fluorescence) in cre-expressing neurons

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP-CCR5TC

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1A

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-sgGFP-NT1

Plasmid

#46914

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1)

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

sgGFP-NT1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-WT-VWF

Plasmid

#124794

Purpose

Expresses multimeric human VWF with C-terminal c-Myc tag

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF (VWF Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCB

Plasmid

#162699

Purpose

Expresses pHnCB10 derived carboxysome operon and prk

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

pHnCB10 derived carboxysome operon, prk

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgLKB1 clone1

Plasmid

#162125

Purpose

Lentiviral sgRNA plasmid targeting human LKB1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgLKB1 (STK11 Human)

Use

Lentiviral

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pNoRBP.iGlucoSnFR2

Plasmid

#244104

Purpose

Bacterial expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-Mito-TurboID

Plasmid

#200963

Purpose

3rd gen lentiviral plasmid for expressing mitochondria localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

Mito-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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