We narrowed to 18,889 results for: Tec

1. pX330A_dCas9-1x2

   Plasmid

   #63596

   Purpose

   Expresses dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   humanized S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D1361Y

   Promoter

   CBh

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. YEp181-CUP1-His-Smt3

   Plasmid

   #99536

   Purpose

   Yeast episomal vector for expression of His-tagged Smt3 (SUMO); LEU2 marker

   Depositor

   Helle Ulrich

   Article

   Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

   Insert

   SMT3 (SMT3 Budding Yeast)
   

   Tags

   7xHis

   Expression

   Yeast

   Promoter

   CUP1

   Available Since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-L1-PGK-HygTK-L2

   Plasmid

   #141006

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   HygTK
   

   Expression

   Mammalian

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBT373

   Plasmid

   #125614

   Purpose

   Expresses FERNY-BE4max-NG in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   FERNY-BE4max-NG
   

   Expression

   Mammalian

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-Neq2X

   Plasmid

   #182365

   Purpose

   Expresses Neq2X DNA polymerase

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

   Insert

   Neq2X
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBLO1811_Cas9_noNLS_human

   Plasmid

   #74490

   Purpose

   Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   Cas9
   

   Expression

   Mammalian

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. GB4070

   Plasmid

   #187811

   Purpose

   Module for the constitutive expression of Cup2:Gal4AD and gRNA-1 DFR, and the copper-inducible expression of dCasEV2.1 (dCas9:EDLL and MS2:VPR).

   Depositor

   Diego Orzaez

   Article

   Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

   Insert

   copper-inducible dCasEV2.1
   

   Expression

   Plant

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBLO1808_arC9_2xNLS_human

   Plasmid

   #74491

   Purpose

   Human codon optimized plasmid to express arC9 t2a mCherry w/2xNLS and a sgRNA

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   arC9
   

   Tags

   t2a mCherry

   Expression

   Mammalian

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMR-A-6

   Plasmid

   #138520

   Purpose

   P3d

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   T7-RNAP
   

   Use

   Synthetic Biology

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Gata4-GFP donor

    Plasmid

    #113115

    Purpose

    Donor plasmid for knock-in eGFP fusing to the c-terminal of mouse Gata4 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Gata4 donor region including homology arms and eGFP
    

    Use

    Synthetic Biology

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tet-O-FUW-Olig2

    Plasmid

    #45844

    Depositor

    Marius Wernig

    Article

    Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

    Insert

    Olig2 (Olig2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    tet-ON

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. BiP-mGFP P495L

    Plasmid

    #62232

    Purpose

    Mutant of hamster BiP-mGFP unable to bind clients.

    Depositor

    Erik Snapp

    Article

    Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

    Insert

    hamster BiP-mGFP-KDEL
    

    Tags

    mGFP

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTKhc-yibDp-GFP-cp6

    Plasmid

    #134938

    Purpose

    Low phosphate inducible circular permutation of GFP

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    GFPcp6
    

    Tags

    His tag

    Expression

    Bacterial

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCHERRY10

    Plasmid

    #24664

    Depositor

    Tanya Parish

    Article

    Carroll et al PLoS One. 2010 . 5(3):e9823.

    Insert

    mCherry
    

    Expression

    Bacterial

    Available Since

    July 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. PlayBack-Ef1a-Bamh1

    Plasmid

    #203308

    Purpose

    For expression of eukaryotic genes of interest with Ef1a promoter with Bamh1 as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCfB4787

    Plasmid

    #106147

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac StrepII msfGFP TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-Rgfp)

    Plasmid

    #55221

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Tags

    StrepII and msfGFP

    Expression

    Insect

    Available Since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFW11-ffluc

    Plasmid

    #128487

    Purpose

    Allelic exchange plasmid to to integrate firefly luciferase gene into S. pyogenes for biophotonic imaging

    Depositor

    Thomas Proft

    Article

    Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

    Insert

    firefly luciferase gene
    

    Use

    Allelic replacement vector

    Tags

    spy0535 downstream flanking region and spy0535 up…

    Promoter

    synthetic

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTW195b63

    Plasmid

    #136931

    Purpose

    Directed evolution of SpCas11

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    gIII(N)-npuN
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. apt-CBE-3'end

    Plasmid

    #219939

    Purpose

    Mammalian expression of apt-CBE-3'end protein (MCP_32aa-linker_evoAPOBEC1) with P2A-EGFP marker

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    MCP_32-aa_evo-rA1_2xUGI
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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