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We narrowed to 8,064 results for: gnal

Showing: 4981 - 5010 of 8064 results

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pET28-p53 (1-90) (72P)

Plasmid

#62080

Purpose

Expression of human p53 (1-90) (72P) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

contains TAD/PP residues 1-90; 72P

Promoter

T7

Available since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6

Plasmid

#192788

Purpose

aGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

Depositor

Jacob Piehler

Article

Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

Insert

antiGFP nanobody enhancer

Use

Tags

H6 and ybbR

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Fzd6

Plasmid

#123588

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

frizzled class receptor 6 (Fzd6 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2 Pc-gA

Plasmid

#51255

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 216,220,224,230,248 mutated to Ala

Promoter

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
HMGN1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176943

Purpose

Mammalian expression vector encoding HMGN1 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

HMGN1 (HMGN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag Grb10 9A long

Plasmid

#37485

Depositor

David Sabatini

Article

Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

Insert

Grb10 9A (GRB10 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

isoform A, 594aa, S104A S150A T155A S158A S426A S…

Promoter

Available since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
pWZLblasti-HA-MEK1 C121S

Plasmid

#53165

Purpose

Expresses HA tagged human MEK1 C121S from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

C121S

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A

Plasmid

#51257

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248,789,805,817,848,923,956 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 216,220,224,230,248,789,805,817,848,9…

Promoter

EF1a

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-Venus-cpVenus-FLARE-EKAR-EV

Plasmid

#123339

Purpose

Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-cpVenus-FLARE-EKAR-EV

Use

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCWXPGR-pTF-DVL2deltaDIX

Plasmid

#114276

Purpose

TET-inducible expression of DVL2 protein with a deletion of its DIX-domain

Depositor

Patrick Salmon

Article

Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

Insert

DVL2 (Dvl2 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

deletion of DIX domain

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2 Pc-gE

Plasmid

#51256

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Glu

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 216,220,224,230,248 mutated to Glu

Promoter

EF1a

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pCMV4Neo-murine IL-17RASEFIR-HA

Plasmid

#46863

Purpose

expresses mouse IL-17RA with SEFIR deletion

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RA-delta-SEFIR (Il17ra Mouse)

Use

Tags

3x HA

Expression

Mammalian

Mutation

Deletion of SEFIR domain (AA 394-485)

Promoter

CMV

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pPGK-T7/2-CD44v9 (-cyt)

Plasmid

#137819

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v9 (-cyt) (CD44 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region

Promoter

PGK

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-Venus-cpVenus-FLARE-AKAR

Plasmid

#123337

Purpose

Plasma membrane-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Lyn-Venus-cpVenus-FLARE-AKAR

Use

Tags

N-terminal targeting sequence from Lyn kinase, Ve…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO SnRK2.2 (20-362)

Plasmid

#177845

Purpose

Bacterial Expression of SnRK2.2

Depositor

Karsten Melcher

Article

Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

SNF1-related protein kinase 2 (SNRK2.2 Mustard Weed)

Use

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-cpVenus-FLARE-CKAR

Plasmid

#123343

Purpose

Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-cpVenus-FLARE-CKAR

Use

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMT-ubb-NLS-BirA-2a-mCherry

Plasmid

#79886

Purpose

Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Tags

3x HA

Expression

Bacterial

Mutation

Promoter

Ubiquitin

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-FLCN (C terminus)

Plasmid

#72297

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FLCN C-terminus (FLCN Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Contains only C terminus of FLCN as defined in PM…

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
3xNLS-NLP-cMyc-cMyc enAspCas12a

Plasmid

#182127

Purpose

pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

3xNLS-NLP-cMyc-cMyc enAspCas12a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-L3F6H

Plasmid

#177765

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2 P1-4A

Plasmid

#51252

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 789,805,817,848 mutated to Ala

Promoter

EF1a

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP Kind2 S181E

Plasmid

#105312

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S181E

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S181A

Plasmid

#105307

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S181A

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 beta4 1355T

Plasmid

#16038

Depositor

Filippo Giancotti

Article

Nikolopoulos et al Mol Cell Biol. 2005 Jul . 25(14):6090-10

Insert

beta4 (ITGB4 Human)

Use

Tags

Expression

Mammalian

Mutation

1355T: amino acids 1-1355

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pLKO.1puro-shKIBRA-A

Plasmid

#40888

Depositor

Paul Reynolds

Article

Moleirinho et al Oncogene. 2012 May 21. doi: 10.1038/onc.

Insert

shKIBRA-A (WWC1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only
pLKO.1puro-shKIBRA-B

Plasmid

#40889

Depositor

Paul Reynolds

Article

Moleirinho et al Oncogene. 2012 May 21. doi: 10.1038/onc.

Insert

shKIBRA-B (WWC1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

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pCR3-FLAG-Gephyrin G

Plasmid

#68817

Purpose

Geph G domain expression in mamalian cells

Depositor

Shiva Tyagarajan

Article

Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

Insert

Gephyrin (Gphn Rat)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

NIL

Promoter

CMV

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only
pCS2-zfIl34

Plasmid

#168112

Purpose

Express zebrafish Il34, mRNA synthesis

Depositor

Petr Bartunek

Article

Hason et al Blood Adv. 2022 Jan 3. pii: 483305. doi:

Insert

Il34 (il34 Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/GFPZNF598-9P-9A

Plasmid

#141192

Purpose

Expresses GFP-ZNF598 mutated in polyproline streches in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

ZNF598 (ZNF598 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

all 3 proline repeats are mutated to Alanine (9xP…

Promoter

CMV

Available since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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GET1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176985

Purpose

Mammalian expression vector encoding GET1 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

GET1 (GET1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge