We narrowed to 7,778 results for: CCH
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Plasmid#59576PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection markerDepositorInsertTwin-Strep tag optimized for PCR amplification followed by URA3 selection marker
UseE. coli cloning vectorTagsTwin-Strep tagPromoterNoneAvailable SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCfB3039(XII-2 MarkerFree)
Plasmid#73279PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-2 (Chr XII: 808805..809939)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAVA-Gal11p-LGF2
Plasmid#127480PurposePositive control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (RNAp associated) interactionDepositorInsertGal11p-LGF2 (GAL11 Budding Yeast)
ExpressionBacterialAvailable SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBBK86 pHomL-RPL18Bp-preCOX4-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179070PurposeVector containing a yeast integration cassette for RPL18B-driven expression of preCOX4-Link-Scarlet(rfp), a marker for the mitochondriaDepositorInsertRPL18Bp-preCOX4-Link-Scarlet(rfp)
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK68 pHomL-RPL18Bp-SRM1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179052PurposeVector containing a yeast integration cassette for RPL18B-driven expression of SRM1-Link-Scarlet(rfp), a marker for the nucleusDepositorInsertRPL18Bp-SRM1-Link-Scarlet(rfp)
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBMN_HA-TBK1 (E696K)
Plasmid#199207PurposeUsed for the expression of TBK1 carrying E696K mutation (SMC Internal No. 1998)DepositorAvailable SinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA0055
Plasmid#131774PurposeGuide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.DepositorInsert20mer CASS5a guide (gCASS5a) and 5' sgRNA
ExpressionBacterial and YeastAvailable SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
SNAP-MIDAS
Plasmid#171604PurposeExpresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) in bacteria.DepositorInsertMIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
TagsHis6 tag and SNAP tagExpressionBacterialAvailable SinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBBK51 pHomL-RPL18Bp-Neon(gfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)
Plasmid#179035PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-SCS2tmh, a marker for the endoplasmic reticulumDepositorInsertRPL18Bp-Neon(gfp)-Link-SCS2tmh
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB3036(XI-1 MarkerFree)
Plasmid#73274PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-1 (Chr XI: 67491..68573)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRpr150-LUC
Plasmid#85700Purposeluciferase reporter driven by a DNA damage responsive cis-regulatory sequence from the reaper locus, containing a p53 binding site (Rpr150 enhancer)DepositorInsertRpR 150 enhancer and hsp70 minimal promoter
UseLuciferaseTagsluciferaseExpressionMammalianPromoterRpR 150 enhancer and hsp70 minimal promoterAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBBK92 pHomL-TDH3p-Scarlet(rfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)
Plasmid#179076PurposeVector containing a yeast integration cassette for expressing TDH3p-Scarlet(rfp)-Link-SCS2tmh, an overexpressed (bright) marker for the endoplasmic reticulumDepositorInsertTDH3p-Scarlet(rfp)-Link-SCS2tmh
ExpressionYeastAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK87 pHomL-RPL18Bp-TOM70-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179071PurposeVector containing a yeast integration cassette for RPL18B-driven expression of TOM70-Link-Scarlet(rfp), a marker for the mitochondriaDepositorInsertRPL18Bp-TOM70-Link-Scarlet(rfp)
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK85 pHomL-RPL18Bp-PEX25-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179069PurposeVector containing a yeast integration cassette for RPL18B-driven expression of PEX25-Link-Scarlet(rfp), a marker for the peroxisomeDepositorInsertRPL18Bp-PEX25-Link-Scarlet(rfp)
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK81 pHomL-RPL18Bp-PRC1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179065PurposeVector containing a yeast integration cassette for RPL18B-driven expression of PRC1-Link-Scarlet(rfp), a marker for the vacuoleDepositorInsertRPL18Bp-PRC1-Link-Scarlet(rfp)
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK78 pHomL-RPL18Bp-SNF7-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179062PurposeVector containing a yeast integration cassette for RPL18B-driven expression of SNF7-Link-Scarlet(rfp), a marker for the endosome, class E compartmentDepositorInsertRPL18Bp-SNF7-Link-Scarlet(rfp)
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK77 pHomL-RPL18Bp-Scarlet(rfp)-Link-VPS21-SSA1t-HomR (AmpR)
Plasmid#179061PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-VPS21, a marker for the endosomesDepositorInsertRPL18Bp-Scarlet(rfp)-Link-VPS21
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK74 pHomL-RPL18Bp-Scarlet(rfp)-Link-SFT2-SSA1t-HomR (AmpR)
Plasmid#179058PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SFT2, a marker for the late GolgiDepositorInsertRPL18Bp-Scarlet(rfp)-Link-SFT2
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK73 pHomL-RPL18Bp-Scarlet(rfp)-Link-SED5-SSA1t-HomR (AmpR)
Plasmid#179057PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SED5, a marker for the early GolgiDepositorInsertRPL18Bp-Scarlet(rfp)-Link-SED5
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK72 pHomL-RPL18Bp-Scarlet(rfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)
Plasmid#179056PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SCS2tmh, a marker for the endoplasmic reticulumDepositorInsertRPL18Bp-Scarlet(rfp)-Link-SCS2tmh
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK71 pHomL-RPL18Bp-ARC35-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179055PurposeVector containing a yeast integration cassette for RPL18B-driven expression of ARC35-Link-Scarlet(rfp), a marker for the actin cytoskeletonDepositorInsertRPL18Bp-ARC35-Link-Scarlet(rfp)
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK69 pHomL-RPL18Bp-SPC42-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179053PurposeVector containing a yeast integration cassette for RPL18B-driven expression of SPC42-Link-Scarlet(rfp), a marker for the spindle poleDepositorInsertRPL18Bp-SPC42-Link-Scarlet(rfp)
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a SVEM hphMX6 lys1-I-SceI cleavage site
Plasmid#67648PurposeI-SceI cleavage site at lys1-hphMX6DepositorInsertsUseTo test i-scei cleavage efficiencyAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS315-48nt-telo-I-SceI
Plasmid#67623Purposetelomere cassette for telomere formation system containing 48 bp of S. pombe telomere repeatsDepositorInsertsUseYeast cen/ars plasmidAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBBK49 pHomL-RPL18Bp-Neon(gfp)-Link-TUB1-SSA1t-HomR (AmpR)
Plasmid#179033PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-TUB1, a marker for the microtubulesDepositorInsertRPL18Bp-Neon(gfp)-Link-TUB1
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-FSF1
Plasmid#110641PurposeRetroviral expression of flag-tagged yeast FSF1 in mammalian cellsDepositorInsertFSF1 (FSF1 Budding Yeast)
UseRetroviralTagsFLAGExpressionMammalianMutationcodon-optimized cDNA (no amino acid mutations)Available SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(-) + SLNCR1 _701-902
Plasmid#86821Purposemammalian expression of lncRNA LINC00673/SLNCR1 deletion constructDepositorAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDN-NG1Tt
Plasmid#44523DepositorInsertsUseSynthetic Biology; Expression regulator/reporterExpressionYeastPromoterPGal1 (wt)Available SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
DT050A
Plasmid#159411PurposeConstitutive murine mature BDNF expression, controlled under E.coli J23101 (strong) promoterDepositorInsertpr.J23101-BDNF (Bdnf, Mouse) (Bdnf Synthetic, Mouse)
UseSynthetic BiologyTagsFLAGExpressionBacterialPromoterJ23101 promoterAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS306Sup35R1
Plasmid#17715DepositorInsertpRS306Sup35R1 (SUP35 Budding Yeast)
ExpressionYeastMutationAn pRS306 based integrating plasmid designed for …Available SinceApril 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFL His-SDS22
Plasmid#113512PurposeExpression of His-SDS22 in insect cells (Baculovirus system)DepositorAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBacPAK8-His-myc-Uba1
Plasmid#29508DepositorAvailable SinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB401+Citrine
Plasmid#128432PurposeModified R4pGWB401 three way Gateway destination vector (Nakagawa et al. 2008) that enables C-terminal fusion of Citrine, a dimeric acid stable yellow fluorescent protein.DepositorTypeEmpty backboneUseGateway destination vectorTagsCitrine, yellow fluorescence proteinExpressionPlantAvailable SinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ABKAR-Kras
Plasmid#84637PurposeUnimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targetedDepositorInsertCerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras
Tags6xHis, C-terminal targeting sequence from Kras, C…ExpressionMammalianPromoterCMVAvailable SinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAEL007584-Cas9
Plasmid#100706PurposeThe promoter of AAEL007584 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL007584-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectPromoterAAEL007584 and Opie2Available SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNIA-CEN-Venus-FLAG-LINXa4-Bre1
Plasmid#73618PurposeVisualizing the nuclear translocation of LINXa4-Bre1 in yeast.DepositorInsertVenus
UseSynthetic BiologyTagsFLAGExpressionYeastMutationBre1 point mutations K8A, K9A and K11APromoterADH1Available SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(-) + SLNCR1 _462-699
Plasmid#86819Purposemammalian expression of lncRNA LINC00673/SLNCR1 deletion constructDepositorAvailable SinceMarch 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(-) + SLNCR1 _568-637
Plasmid#86820Purposemammalian expression of lncRNA LINC00673/SLNCR1 deletion constructDepositorAvailable SinceMarch 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDSM-de-Pfba1-PYC-Trpl15a
Plasmid#127738PurposeYeast pathway position 5. PYC transcription unit with the FBA1 promoter and RPL15A terminator.DepositorInsertpyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
UseSynthetic BiologyExpressionYeastPromoterPfba1Available SinceJan. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-ODC2
Plasmid#133257PurposeExpress FLAG-tagged ODC2 in mammalian cellsDepositorInsertODC2 (ODC2 Budding Yeast)
UseRetroviralTags3xFLAGExpressionMammalianMutationcodon-optimized for expression in human cellsAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only