We narrowed to 22,820 results for: Fus

1. pET SUMO-syt7 C2AB-2A

   Plasmid

   #213627

   Purpose

   Bacterial expression of SUMO-tagged mouse syt7 C2AB-2A

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt7 C2AB-2A (Syt7 Mouse)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   I298, L361A

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET SUMO-FL-syt1 5W

   Plasmid

   #213629

   Purpose

   Bacterial expression of SUMO-tagged rat FL-syt1 5W

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   FL-syt1 5W (Syt1 Rat)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   M173W, F231W, F234W, C277A, V304W, Y364W, I367C

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1 NKX6-2-Venus R200W

   Plasmid

   #209179

   Purpose

   Mammalian expression of SPAX8-related missense mutation (R200W) in human NKX6-2 fused with the Venus fluorescent protein

   Depositor

   Federico Herrera

   Article

   Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

   Insert

   NKX6-2 (R200W) fused with Venus (NKX6-2 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   NKX6-2 (R200W)

   Promoter

   CMV

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1 NKX6-2-Venus L163V

   Plasmid

   #209178

   Purpose

   Mammalian expression of SPAX8-related missense mutation (L163V) in human NKX6-2 fused with the Venus fluorescent protein

   Depositor

   Federico Herrera

   Article

   Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

   Insert

   NKX6-2 (L163V) fused with Venus (NKX6-2 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   NKX6-2 (L163V)

   Promoter

   CMV

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pminiTol2-4kb wnt3 promoter-PMTmApple

   Plasmid

   #203794

   Purpose

   Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of PMTmApple for zebrafish transgenesis

   Depositor

   Thorsten Wohland

   Article

   Veerapathiran et al Elife. 2020 Nov 25;9:e59489. doi: 10.755

   Insert

   PMT
   

   Use

   Zebrafish expression

   Tags

   mApple

   Expression

   Mutation

   Promoter

   4kb Wnt3 promoter

   Available since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p3xFLAG-CMV-14-Mfn1-T564A

   Plasmid

   #191978

   Purpose

   To produce mutants mimicking dephosphorylated Mfn1

   Depositor

   Luca Scorrano

   Article

   Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

   Insert

   Mfn1 T564A (Mfn1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T564A

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p3xFLAG-CMV-14-Mfn1-T564D

   Plasmid

   #191979

   Purpose

   To produce mutants mimicking phosphorylated Mfn1

   Depositor

   Luca Scorrano

   Article

   Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

   Insert

   Mfn1 T564D (Mfn1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T564D

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pfa6a mScarlet-FKBP::KANMX

   Plasmid

   #185905

   Purpose

   Plasmid for cloning mScarlet fused to FKBP into yeast.

   Depositor

   Matthew Good

   Article

   Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

   Insert

   mScarlet-FKBP
   

   Use

   Tags

   mScarlet fused to FKBP

   Expression

   Yeast

   Mutation

   Promoter

   SP6

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPBO.ACT002

   Plasmid

   #182712

   Purpose

   aTc inducible dCasRx-IF1

   Depositor

   Joseph Schoeniger

   Article

   Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

   Insert

   dCasRx
   

   Use

   CRISPR

   Tags

   3x(GGGS)-Escherichia Coli Initiation Factor 1

   Expression

   Bacterial

   Mutation

   Catalytically deactivated R295A, H300A, R849A, H8…

   Promoter

   pTet

   Available since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPBO.ACT004

    Plasmid

    #182714

    Purpose

    Constituitvely expressed CasRx crRNA cloning vector, extended 3' terminator region

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    CasRx crRNA cloning backbone
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pfa6a mScarlet-TsCC(B)::KANMX

    Plasmid

    #176397

    Purpose

    Plasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    mScarlet-TsCC(B)
    

    Use

    Tags

    mScarlet fused to TsCC(B)

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    none

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGB-Co-NF-kB

    Plasmid

    #180206

    Purpose

    Expression GAL4-fusion of Co-NF-kB in yeast

    Depositor

    Thomas Gilmore

    Article

    Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

    Insert

    Co-NF-kB
    

    Use

    Tags

    GAL4 (1-147) fusion

    Expression

    Yeast

    Mutation

    aa 2-1224

    Promoter

    AdhI

    Available since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MUM2SP-Citrine

    Plasmid

    #181963

    Purpose

    MUM2 signal peptide fused to Citrine, under control of MUM4_1.5kb promoter

    Depositor

    George Haughn

    Article

    Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

    Insert

    MUM2 signal peptide, fused to the Citrine YFP in pAD
    

    Use

    Tags

    Citrine YFP

    Expression

    Plant

    Mutation

    Promoter

    MUM4pro 1.5kb fragment

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFX-H2B-Sirius

    Plasmid

    #175837

    Purpose

    Express Sirius with nucleus-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Sirius with nucleus-targeting sequence
    

    Use

    Tags

    H2B (human histone 2B)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFX-Sirius-KRasCT

    Plasmid

    #175831

    Purpose

    Express Sirius with plasma membrane-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Sirius with plasma membrane-targeting sequence
    

    Use

    Tags

    C-terminal hypervariable region of K-Ras 4B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFX-SECFP-KRasCT

    Plasmid

    #175832

    Purpose

    Express SECFP with plasma membrane-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    SECFP with plasma membrane-targeting sequence
    

    Use

    Tags

    C-terminal hypervariable region of K-Ras 4B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFX-Lifeact-EGFP

    Plasmid

    #174477

    Purpose

    Express EGFP with actin-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    EGFP with actin-targeting sequence
    

    Use

    Tags

    Lifeact (N-terminal 17 amino acids from S. Cerevi…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFX-SECFP-Sec61β

    Plasmid

    #174465

    Purpose

    Express SECFP with endoplasmic reticulum-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    SECFP with endoplasmic reticulum-targeting sequence
    

    Use

    Tags

    Sec61 subunit beta

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFX-iRFP-KRasCT

    Plasmid

    #175836

    Purpose

    Express iRFP with plasma membrane-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    iRFP with plasma membrane-targeting sequence
    

    Use

    Tags

    C-terminal hypervariable region of K-Ras 4B

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFX-Lifeact-SECFP

    Plasmid

    #174476

    Purpose

    Express SECFP with actin-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    SECFP with actin-targeting sequence
    

    Use

    Tags

    Lifeact (N-terminal 17 amino acids from S. Cerevi…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFX-Lifeact-Sirius

    Plasmid

    #174475

    Purpose

    Express Sirius with actin-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Sirius with actin-targeting sequence
    

    Use

    Tags

    Lifeact (N-terminal 17 amino acids from S. Cerevi…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFX-Tom20-Sirius

    Plasmid

    #174184

    Purpose

    Express Sirius with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Sirius with mitchondria-targeting sequence
    

    Use

    Tags

    the mitochondria-targeting sequence of TOM20

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFX-Sirius-Sec61β

    Plasmid

    #174464

    Purpose

    Express Sirius with endoplasmic reticulum-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Sirius with endoplasmic reticulum-targeting sequence
    

    Use

    Tags

    Sec61 subunit beta

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFX-GalT-Sirius

    Plasmid

    #174469

    Purpose

    Express Sirius with golgi apparatus-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Sirius with golgi apparatus-targeting sequence
    

    Use

    Tags

    β1,4-galactose transferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPLN-hFluc-WT-eGFP-KDEL

    Plasmid

    #177727

    Purpose

    Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminus

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    hFluc
    

    Use

    Tags

    KDEL, Prolactin Signal Sequence, and eGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMP71-mGFP-LLO-hPMEL

    Plasmid

    #174607

    Purpose

    expresses palmitoylated GFP with a C terminal fusion of the LLO190 epitope and the human hPMEL epitope

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    expresses palmitoylated GFP w/C-term fusion of the LLO190 epitope and human hPMEL epitope
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV-FLAG-PGRMC-Hybrid1

    Plasmid

    #173469

    Purpose

    N-terminal region of PGRMC2 fused to the transmembrane and C-terminal region of PGRMC1

    Depositor

    Bill Tsai

    Article

    Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

    Insert

    PGRMC1/PGRMC2 fusion (PGRMC1, PGRMC2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-FLAG-PGRMC-Hybrid2

    Plasmid

    #173470

    Purpose

    N-terminal and transmembrane region of PGRMC2 fused to the C-terminal region of PGRMC1

    Depositor

    Bill Tsai

    Article

    Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

    Insert

    PGRMC1/PGRMC2 fusion (PGRMC1, PGRMC2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-FLAG-PGRMC-Hybrid3

    Plasmid

    #173471

    Purpose

    N-terminal region of PGRMC1 fused to the transmembrane and C-terminal region of PGRMC2

    Depositor

    Bill Tsai

    Article

    Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

    Insert

    PGRMC1/PGRMC2 fusion (PGRMC1, PGRMC2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCA528-PPxY1-GS-WW3e

    Plasmid

    #166705

    Purpose

    expresses an N-terminal fusion of AMOT PPxY1 to NEDD4L WW3 in bacterial cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    AMOT PPxY1 fused to NEDD4L WW3
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. C514-E54: attB5r-RBS-His6-Halotag-nostop-attB1r

    Plasmid

    #162965

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal His6-Halotag-tagged fusion proteins in bacterial expression

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-Halotag fusion tag with upstream E. coli ribosome binding site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. C514-E51: attB5r-RBS-His6-MBP-nostop-attB1r

    Plasmid

    #162964

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal His6-MBP-tagged fusion proteins in bacterial expression

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-Maltose-binding protein fusion tag with upstream E. coli ribosome binding site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. C514-E15: attB5r-RU5u-His6-MBP-nostop-attB1r

    Plasmid

    #162957

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-Maltose-binding protein fusion tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. C514-E13: attB5r-RU5u-His6-GST-nostop-attB1r

    Plasmid

    #162955

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-Glutathione-S-transferase fusion tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. C514-E12: attB5r-RU5u-His6-eGFP-nostop-attB1r

    Plasmid

    #162954

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal eGFP-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-Green fluorescent protein fusion tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. p3E-U6a-U6c-mfn2 guide

    Plasmid

    #121993

    Purpose

    An entry vector with U6a and U6c promoter driving mfn2 guide RNAs expression

    Depositor

    Qing Deng

    Article

    Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

    Insert

    mfn2 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p3E-U6a-U6c-mfn2 guide#2

    Plasmid

    #121994

    Purpose

    An entry vector with U6a and U6c promoter driving mfn2 guide RNAs expression

    Depositor

    Qing Deng

    Article

    Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

    Insert

    mfn2 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. p3E-U6a-U6c-opa1 guide

    Plasmid

    #121995

    Purpose

    An entry vector with U6a and U6c promoter driving opa1 guide RNAs expression

    Depositor

    Qing Deng

    Article

    Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

    Insert

    opa1 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET_4E2H.210

    Plasmid

    #155212

    Purpose

    Expresses 4E2H.210 de novo protein in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    4E2H.210
    

    Use

    Tags

    6x His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBz172_CsoS2_N1_GFP

    Plasmid

    #140852

    Purpose

    His-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeat 1 fused to GFP
    

    Use

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBz175_CsoS2_N4_GFP

    Plasmid

    #140855

    Purpose

    His-tagged fourth N-peptide of NTD fused to Superfolder GFP ([N4]-GFP) used for BLI, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeat 4 fused to GFP
    

    Use

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pBz173_CsoS2_N2_GFP

    Plasmid

    #140853

    Purpose

    His-tagged second N-peptide of NTD fused to Superfolder GFP ([N2]-GFP) used for BLI, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeat 2 fused to GFP
    

    Use

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pBz174_CsoS2_N3_GFP

    Plasmid

    #140854

    Purpose

    His-tagged third N-peptide of NTD fused to Superfolder GFP ([N3]-GFP) used for BLI, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeat 3 fused to GFP
    

    Use

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLz26_CsoS2_N1-N2_GFP

    Plasmid

    #140851

    Purpose

    His-tagged first two N-peptides of NTD fused to Superfolder GFP ([N1-N2]-GFP) used for MST, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeats 1-2 fused to GFP
    

    Use

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pSLQ8636 PB-PGK-RR12EE345L-C term (406) dLbCpf1-N intein-pA

    Plasmid

    #140223

    Purpose

    Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activator

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to N-intein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    PGK

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pRS424-ysg(G:H)

    Plasmid

    #138257

    Purpose

    Expression of GFP-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequence to be used in yeast dual reporter system

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    GFP targeting sgRNAs G and H
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pUC19-hsg(G:H)

    Plasmid

    #138259

    Purpose

    Expression of humanized mCherry-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequences to be used in Traffic Light reporter system

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    mCherry targeting sgRNAs G and H
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCB6-Occludin

    Plasmid

    #136637

    Purpose

    Expression of Occludin in mammalian cells

    Depositor

    Maria Balda, Karl Matter

    Article

    Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

    Insert

    Occludin (OCLN Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCB6-HAOccludin

    Plasmid

    #136638

    Purpose

    Expression of HAOccludin in mammalian cells

    Depositor

    Maria Balda, Karl Matter

    Article

    Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

    Insert

    HAOccludin (OCLN Chicken)
    

    Use

    Tags

    HA (MQDLPGNDNSTAG)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCB6-OccludinCT3

    Plasmid

    #136639

    Purpose

    Expression of OccludinCT3 in mammalian cells

    Depositor

    Maria Balda, Karl Matter

    Article

    Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

    Insert

    OccludinCT3 (OCLN Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal cytoplasid domain

    Promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge