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Showing: 8301 - 8350 of 79968 results

  1. pCDH-EF1-DIO-tdTomato

    Plasmid
    #72254
    Purpose
    Express tdTomato under EF-1 promoter when Cre is expressed by another vector
    Depositor
    Kazuhiro Oka
    Insert
    tdTomato
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Promoter
    elongation factor-1
    Available Since
    Jan. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    72254-plasmid-map-sequence-id-134462

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  2. pcDNA5-FRT-TOgw-C-mEGFP

    Plasmid
    #223953
    Purpose
    Gateway cloning of genes of interest with a C-terminal mEGFP fusion; generation of stable cells with doxycycline inducible expression
    Depositor
    Karla Feijs, Roko Žaja
    Type
    Empty backbone
    Tags
    mEGFP
    Expression
    Mammalian
    Available Since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    223953-plasmid-map-sequence-id-450835

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  3. pcDNA5-FRT-TOgw-C-mECFP

    Plasmid
    #223950
    Purpose
    Gateway cloning of genes of interest with a C-terminal mECFP fusion; generation of stable cells with doxycycline inducible expression
    Depositor
    Karla Feijs, Roko Žaja
    Type
    Empty backbone
    Tags
    mECFP
    Expression
    Mammalian
    Available Since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    223950-plasmid-map-sequence-id-450833

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  4. pcDNA5-FRT-TOgw-C-mEYFP

    Plasmid
    #223951
    Purpose
    Gateway cloning of genes of interest with a C-terminal mEYFP fusion; generation of stable cells with doxycycline inducible expression
    Depositor
    Karla Feijs, Roko Žaja
    Type
    Empty backbone
    Tags
    mEYFP
    Expression
    Mammalian
    Available Since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    223951-plasmid-map-sequence-id-450832

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  5. pcDNA5-FRT-TOgw-N-mEGFP

    Plasmid
    #223949
    Purpose
    Gateway cloning of genes of interest with an N-terminal mEGFP fusion; generation of stable cells with doxycycline inducible expression
    Depositor
    Karla Feijs, Roko Žaja
    Type
    Empty backbone
    Tags
    mEGFP
    Expression
    Mammalian
    Available Since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    223949-plasmid-map-sequence-id-450830

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  6. pOpen-K12polLF

    Plasmid
    #165511
    Purpose
    DNA pol fragment that retains 5'-3' polymerase activity and 3'-5' exonuclease activity but loses 5'-3' exonuclease activity.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    DNA Polymerase I, Large (Klenow) Fragment
    Use
    Synthetic Biology
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165511-plasmid-map-sequence-id-338596

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  7. Dendra2-NS3

    Plasmid
    #238004
    Purpose
    Expresses NS3 in mammalian/mouse cell line
    Depositor
    Partha Mondal
    Insert
    NS3
    Tags
    Dendra2
    Expression
    Mammalian
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    238004-plasmid-map-sequence-id-474227

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  8. HA-IST1

    Plasmid
    #131619
    Purpose
    Expresses HA-tagged human IST1 in mammalian cells
    Depositor
    FENG-QIAN Li, Ken-Ichi Takemaru
    Insert
    human IST1
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131619-plasmid-map-sequence-id-258201

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  9. pFastBac HTB MBP-SARM1

    Plasmid
    #195763
    Purpose
    Purification of SARM1 from insect cells
    Depositor
    Hao Wu
    Insert
    SARM1 (SARM1 Human)
    Tags
    HIS-MBP-TEV
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195763-plasmid-map-sequence-id-384746

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  10. CRY2olig-FUS

    Plasmid
    #218921
    Purpose
    Expression of CRY2olig-mCherry fused to FUS
    Depositor
    Tatyana Shelkovnikova
    Insert
    CRY2olig-mCherry tagged FUS
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CMV
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    218921-plasmid-map-sequence-id-442218

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  11. pAAV-CaMKII-GFP

    Plasmid
    #64545
    Purpose
    AAV-mediated expression of GFP under the CaMKII promoter.
    Depositor
    Edward Boyden
    Insert
    GFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CaMKII
    Available Since
    Nov. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    64545-plasmid-map-sequence-id-130468

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  12. pR6K-GFP-RFP

    Plasmid
    #199652
    Purpose
    R6K backbone with Golden Gate (GG) cloning sites
    Depositor
    IIya Finkelstein
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    J23119 promoter
    Available Since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    199652-plasmid-map-sequence-id-412250

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  13. pUPD2 CBS4 (GB2718)

    Plasmid
    #160601
    Purpose
    Copper dependent CUP2 binding DNA sequence
    Depositor
    Diego Orzaez
    Insert
    CBS4
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160601-plasmid-map-sequence-id-322066

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  14. pcDNA3-GFP-MMP7

    Plasmid
    #11989
    Depositor
    Steven Johnson
    Insert
    MMP7 (MMP7 Human)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 25, 2007
    Availability
    Academic Institutions and Nonprofits only
    11989-plasmid-map-sequence-id-133484

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  15. pcDNA3.1-N-Luc

    Plasmid
    #185357
    Purpose
    For mammalian expression of N-terminal half of Firefly luciferase for split-Luciferase experiments (amino acids 2-416).
    Depositor
    Ayaz Najafov
    Insert
    Firefly Luciferase
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    June 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    185357-plasmid-map-sequence-id-363972

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  16. pRK5F-ALK5-HA(ecto)

    Plasmid
    #31720
    Depositor
    Rik Derynck
    Insert
    ALK5 (TGFBR1 Human)
    Tags
    FLAG and HA
    Expression
    Mammalian
    Available Since
    Feb. 14, 2012
    Availability
    Academic Institutions and Nonprofits only

  17. pColdII-SIRPT1-Bubalus bubalis

    Plasmid
    #75350
    Purpose
    Nt His-tag Bubalus bubalis Sacsin 86-1364
    Depositor
    Kalle Gehring
    Insert
    Sacsin Bubalus bubalis 86-1364
    Tags
    His-Tag
    Expression
    Bacterial
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    75350-plasmid-map-sequence-id-142906

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  18. pCAGG-NFIB2

    Plasmid
    #112700
    Purpose
    Expression of NFIB2 protein and GFP
    Depositor
    Richard Gronostajski
    Insert
    Nuclear Factor I B2 (Nfib Mouse)
    Tags
    GFP and HA
    Expression
    Mammalian
    Available Since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112700-plasmid-map-sequence-id-212390

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  19. pTRIPZ (M)-HT-NLS

    Plasmid
    #82518
    Purpose
    Lentivirus vector. Expresses Halotag proteins with nuclear localization signal in mammalian cells.
    Depositor
    Xiaojun Ren
    Insert
    Nuclear localizaition sequence
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    82518-plasmid-map-sequence-id-156794

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  22. pLenti6M GLUT9

    Plasmid
    #194144
    Purpose
    Expresses GLUT9 protein in mammalian cells
    Depositor
    Jun Liu
    Insert
    SLC2A9 (SLC2A9 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194144-plasmid-map-sequence-id-380731

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  23. GFAP-MCS-WPRE-SV40 - GCH31

    Plasmid
    #230829
    Purpose
    AAV cloning vector for expression under the GFAP promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230829-plasmid-map-sequence-id-457134

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  24. pUC119-MCS

    Plasmid
    #58807
    Purpose
    contains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.
    Depositor
    Jen Sheen
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Available Since
    July 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    58807-plasmid-map-sequence-id-91105

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  25. pColdII-SIRPT1-Cucumis sativus

    Plasmid
    #75357
    Purpose
    Nt His-tag Cucumis sativus Sacsin 1-1292
    Depositor
    Kalle Gehring
    Insert
    Sacsin Cucumis sativus 1-1292
    Tags
    His-Tag
    Expression
    Bacterial
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    75357-plasmid-map-sequence-id-142885

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  26. pCAGG-NFIC2

    Plasmid
    #112701
    Purpose
    Expression of NFIC2 protein and GFP
    Depositor
    Richard Gronostajski
    Insert
    Nuclear Factor I C2 (Nfic Mouse)
    Tags
    GFP and HA
    Expression
    Mammalian
    Available Since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112701-plasmid-map-sequence-id-212389

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  28. pCDH-EF1-IRES plum

    Plasmid
    #128072
    Purpose
    gfp was exchanged to plum
    Depositor
    Oskar Laur
    Insert
    plum
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    no
    Available Since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128072-plasmid-map-sequence-id-255193

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  29. pRosettav2

    Plasmid
    #115557
    Purpose
    To determine correct antibiotic selection conditions in mammalian cells; standard for titering experiments
    Depositor
    John Doench
    Insert
    Hygromycin-Blasticidin-Puromycin-EGFP
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    115557-plasmid-map-sequence-id-226246

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  30. pcDNA3-TopBP1-mCer

    Plasmid
    #211414
    Purpose
    mammalian expression of human TopBP1 C-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    TopBP1 (TOPBP1 Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211414-plasmid-map-sequence-id-420477

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  31. pCS2+MCS-P2A-sfGFP

    Plasmid
    #74668
    Purpose
    Plasmid for expression of wild-type or mutant cDNA fragments (mutation reporter) or complete cDNAs in frame with superfolderGFP.
    Depositor
    Jason Berman
    Insert
    sfGFP
    Expression
    Mammalian
    Available Since
    May 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    74668-plasmid-map-sequence-id-146800

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  32. pET22B_SA_PcrA

    Plasmid
    #102999
    Purpose
    The plasmid is used to express Staphylococcus aureus PcrA, a DNA helicase, with N-terminal His-tag and Gly-Gly linker
    Depositor
    Martin Webb
    Insert
    SA_PcrA
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    PcrA 199-2423
    Promoter
    T7
    Available Since
    May 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    102999-plasmid-map-sequence-id-199646

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  33. pHMR1B

    Plasmid
    #102958
    Purpose
    gene cloning of MR1B
    Depositor
    Gennaro De Libero, Lucia Mori
    Insert
    MR1B cDNA (MR1P1 Human)
    Use
    Cloning
    Mutation
    Stop codon has been removed by HindIII site
    Available Since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    102958-plasmid-map-sequence-id-198778

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  35. pCDH-CMV-Nluc

    Plasmid
    #73038
    Purpose
    Express Nluc reporter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73038-plasmid-map-sequence-id-136036

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  36. pBOB-Septin 2 -GFP

    Plasmid
    #118734
    Purpose
    To express Septin 2 tagged with GFP on C-terminus
    Depositor
    John Cooper
    Insert
    Septin 2 (SEPTIN2 Human)
    Use
    Lentiviral
    Tags
    GFP
    Promoter
    CMV
    Available Since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    118734-plasmid-map-sequence-id-229082

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  37. pPGS-hSLUG.fl.flag

    Plasmid
    #25696
    Depositor
    Eric Fearon
    Insert
    SLUG (SNAI2 Human)
    Use
    Retroviral
    Tags
    FLAG
    Available Since
    July 9, 2010
    Availability
    Academic Institutions and Nonprofits only

  38. pCDH-CB-copGFP-T2A-iCre

    Plasmid
    #72256
    Purpose
    Express copGFP and iCre under CB promoter
    Depositor
    Kazuhiro Oka
    Insert
    copGFP-T2A-iCre
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CB and chicken beta-actin + CMV enhancer
    Available Since
    Jan. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72256-plasmid-map-sequence-id-134470

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  39. pRSV-PKImut-v2

    Plasmid
    #45067
    Purpose
    Expression of intactive PKI (PKA inhibitor) mutant
    Depositor
    Richard Maurer
    Insert
    cAMP-dependent protein kinase inhibitor alpha
    Expression
    Mammalian
    Mutation
    changed Arg 20 & 21 to Glycines
    Promoter
    RSV
    Available Since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    45067-plasmid-map-sequence-id-62502

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  40. pColdII-SIRPT1-Eptesicus fuscus

    Plasmid
    #78113
    Purpose
    Nt His-tag Eptesicus fuscus Sacsin 89-1368
    Depositor
    Kalle Gehring
    Insert
    Sacsin Eptesicus fuscus 89-1368
    Tags
    His-Tag
    Expression
    Bacterial
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78113-plasmid-map-sequence-id-145659

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  41. pLNCX mouse src

    Plasmid
    #13658
    Depositor
    Joan Brugge
    Insert
    src (Src Mouse)
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    Jan. 4, 2007
    Availability
    Academic Institutions and Nonprofits only

  42. CMV-VGF

    Plasmid
    #107575
    Purpose
    Encodes the entire VGF cDNA sequence under the control of CMV promoter. Allows expression of VGF in mammalian cells.
    Depositor
    Andrea Levi
    Insert
    VGF (Vgf Rat)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107575-plasmid-map-sequence-id-207612

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  43. pHAGE2-EF1aL-dsRed-W

    Plasmid
    #195863
    Purpose
    lentiviral expression of the red fluorescent protein driven by human EF1aL promoter
    Depositor
    Darrell Kotton
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    dsRed
    Promoter
    EF1aL
    Available Since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195863-plasmid-map-sequence-id-383935

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  44. BII-Sh-BnTW

    Plasmid
    #133365
    Purpose
    Piggybac vector for shRNA-mediated knockdown, with co-expression with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Tags
    Flag-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available Since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133365-plasmid-map-sequence-id-268571

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  46. pOT2-poly-cis

    Plasmid
    #141177
    Purpose
    To clone any gene or STTM sequence or artificial miRNA under CaMV 35S promoter
    Depositor
    Guiliang Tang
    Type
    Empty backbone
    Use
    RNAi and Synthetic Biology
    Expression
    Plant
    Promoter
    CaMV 35S promoter
    Available Since
    Oct. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    141177-plasmid-map-sequence-id-299765

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  47. pCFP Paxillin

    Plasmid
    #50510
    Depositor
    Kenneth Yamada
    Insert
    Paxillin (PXN Human)
    Tags
    ECFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  48. pLenti-CaMKIIa-mTagBFP2

    Plasmid
    #87254
    Purpose
    Lentiviral fluorescent reporter using the neuronal cell type-specific promoter of the CaM Kinase II alpha gene.
    Depositor
    James Ellis
    Insert
    mTagBFP2
    Use
    Lentiviral
    Tags
    6xHis
    Promoter
    CaMKIIa
    Available Since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    87254-plasmid-map-sequence-id-174000

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  49. pBOB-Septin 2 -tdTomato

    Plasmid
    #118735
    Purpose
    To express Septin 2 tagged with tdTomato on C-terminus
    Depositor
    John Cooper
    Insert
    Septin 2 (SEPTIN2 Human)
    Use
    Lentiviral
    Tags
    tdTomato
    Promoter
    CMV
    Available Since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    118735-plasmid-map-sequence-id-229277

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  50. pPB-spCas9-pNeo

    Plasmid
    #214120
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    spCas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available Since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    214120-plasmid-map-sequence-id-423617

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Showing: 8301 - 8350 of 79968 results