We narrowed to 13,780 results for: TIM

1. LENC

   Plasmid

   #111163

   Purpose

   miR-E (miR-30 variant)-based RNAi with mCherry reporter

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM6-G6-vioABECD

   Plasmid

   #66536

   Purpose

   pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with G6 promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Inserts

   VioA
   VioB
   VioE
   VioC
   VioD
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Mutant T7 Promoter - G6

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. TagBFP2-C1-SACM1LdeltaTMD-C389S-Fis1tail

   Plasmid

   #220079

   Purpose

   SAC1 with a C389S mutation in it with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue Fluorophore

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

   Insert

   SACM1l-C389S-Fis1tail (SACM1L Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C389SdeltaTMD

   Promoter

   Available since

   May 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pet28a-His6-Halo-Tev-Keap1

   Plasmid

   #62456

   Purpose

   His6-halo tagged human Keap1 protein for E.coli expression

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Keap1 (KEAP1 Human)
   

   Use

   Tags

   His-Halo-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pN006

   Plasmid

   #188470

   Purpose

   Lentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)

   Depositor

   Le Cong

   Article

   Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

   Insert

   AsCas12a
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Sept. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. XE118 CAMKII-T286D-CS2+

   Plasmid

   #16736

   Depositor

   Randall Moon

   Article

   Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

   Insert

   CAMKII-T286D (Camk2a Rat)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   threonine 286 mutated to aspartate

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pN256

   Plasmid

   #188472

   Purpose

   scDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFP

   Depositor

   Le Cong

   Article

   Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

   Insert

   eGFP-WPRE-DAISY-4T
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV500 A-C/EBP

   Plasmid

   #33352

   Depositor

   Charles Vinson

   Article

   Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

   Insert

   C/EBP (Cebpa Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Dominant Negative (see notes)

   Promoter

   CMV

   Available since

   Dec. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.2/NS4A

   Plasmid

   #120826

   Purpose

   Ectopic expression of ZIKV protein

   Depositor

   Jan Rehwinkel

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   NS4A ZIKV
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDIV300

    Plasmid

    #204968

    Purpose

    AMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance marker

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

    Inserts

    Mad7
    hph (hygromycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIVT/Smarca4

    Plasmid

    #122146

    Purpose

    For making Smarca4 RNA

    Depositor

    Carmen Williams

    Article

    Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

    Insert

    Smarca4 (Smarca4 Mouse)
    

    Use

    For in vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGLucIPZ

    Plasmid

    #228703

    Purpose

    Constitutive expression of Gaussia luciferase, puromycin N-acetyl-transferase and shRNAmir of interest

    Depositor

    Thorsten Stiewe

    Article

    Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

    Inserts

    Gaussia luciferase
    non-silencing shRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pFPV25.1_rpsM_mCerulean3

    Plasmid

    #124904

    Purpose

    rpsM promoter (S. typhimurium) driving mCerulean3 (codon-optimized for S. typhimurium)

    Depositor

    Markus Covert

    Article

    Lane et al Cell Syst. 2019 Mar 27;8(3):183-196.e4.

    Insert

    rpsM promoter and mCerulean3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cry2L348F(1-531)-mCh-BAXS184E

    Plasmid

    #117239

    Purpose

    Light activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switch

    Depositor

    Robert Hughes

    Article

    Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

    Insert

    Cry2531.L348F-mCh-BAXS184E
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmKate2-MS2coatProtein

    Plasmid

    #103833

    Purpose

    Live cell reader for MS2 loop RNA production

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    mKate2-MS2 coat protein (cp )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CAG-EBFP2-P2A-EGFP

    Plasmid

    #184938

    Purpose

    Expresses blue fluorescent protein EBFP2 and EGFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    EBFP2-P2A-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.2/NS4B

    Plasmid

    #120823

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    NS4B ZIKV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRU5-inGFPt

    Plasmid

    #60236

    Purpose

    HTLV-1 based transfer vector encoding gfp-turbo reporter gene interrupted by intron

    Depositor

    Dmitriy Mazurov

    Article

    Shunaeva et al J Virol. 2015 Oct 15;89(20):10591-601. d

    Insert

    inGFPt
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pF208wt-luc

    Plasmid

    #122143

    Purpose

    For luciferase expression

    Depositor

    Carmen Williams

    Article

    Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

    Insert

    Fam208a promoter (Tasor Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Fam208a

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Myristoylated optoDroplet

    Plasmid

    #111507

    Purpose

    For membrane localized optoDroplets

    Depositor

    Jared Toettcher

    Article

    Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

    Insert

    Cry2 (CRY2 Mustard Weed)
    

    Use

    Lentiviral

    Tags

    FUS, Fusion Red, and Myristoylation Sequence (MYR…

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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