We narrowed to 9,591 results for: EPO

1. Tol2 8xSTAR-mNeonGreen. PGK-puro

   Plasmid

   #136255

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

   Insert

   stem cell Ascl2 reporter, 8 repeats
   

   Use

   Tol2 transposase

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMVtrunc Affimer6-msfGFPΔN12

   Plasmid

   #231550

   Purpose

   Mammalian expression of Affimer6 fused to N-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   Affimer6
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMVtrunc

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-Actin-Vhh-sfGFP-P2A-1xHA-tdiRFP-caax (JDW 1311)

   Plasmid

   #224497

   Purpose

   Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and tdiRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Actin-Vhh-sfGFP-P2A-HA-tdiRFP-caax
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. A3Ai-Cas9n-UGI-NLS

   Plasmid

   #109425

   Purpose

   Expresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Insertion of an L1 intron into the coding sequenc…

   Promoter

   CMV

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C

   Plasmid

   #154196

   Purpose

   Lentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Insert

   sgRNA-BC1-A, sgRNA-BC1-C
   

   Use

   Lentiviral; Catch barcode activation

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6 and mU6

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHL-H1-ccdB-mEF1a-RiH

   Plasmid

   #60601

   Purpose

   Cloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.

   Depositor

   Akitsu Hotta

   Article

   Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

   Inserts

   Red Fluorescent Protein
   Hygromycin resistance gene
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. CaTCH Dual-sgRNA_sgBC2-A_sgBC2-C

   Plasmid

   #154197

   Purpose

   Lentiviral expression plasmid encoding two sgRNAs without a target. These can be used as a off-target control for CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Insert

   sgRNA-BC2-A, sgRNA-BC2-C
   

   Use

   Lentiviral; Catch barcode activation

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6 and mU6

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAVS1P-iCLHN

   Plasmid

   #66579

   Purpose

   AAVS1 safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-AAVS1 TALENs

   Depositor

   Jizhong Zou

   Article

   Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

   Insert

   Nanoluc-HaloTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-AAVS1

   Plasmid

   #184397

   Purpose

   Donor plasmid for stable integration of firefly luciferase NMD(+) PTC39 reporter at AAVS1

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   Firefly luciferase beta-globin-PTC39
   

   Use

   CRISPR; Donor plasmid for hdr

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   PTC in beta-globin at amino acid position 39

   Promoter

   Tet-On 3G

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGAPTrap-mtagBFP2-IRESMuro

    Plasmid

    #82335

    Purpose

    Vector targeting the mtagBFP2 gene to the GAPDH locus of human cells. BFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    mtagBFP2-IRESMuro
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-AAVS1

    Plasmid

    #184398

    Purpose

    Donor plasmid for stable integration of Renilla luciferase NMD(+) PTC39 reporter at AAVS1

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    Renilla luciferase beta-globin-PTC39
    

    Use

    CRISPR; Donor plasmid for hdr

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    PTC in beta-globin at amino acid position 39

    Promoter

    Tet-On 3G

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC13P-iCLHN

    Plasmid

    #66580

    Purpose

    CLYBL safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-C13 TALENs

    Depositor

    Jizhong Zou

    Article

    Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

    Insert

    Nanoluc-HaloTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHL-EF1a-SphcCas9-iP-A

    Plasmid

    #60599

    Purpose

    Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Insert

    CRISPR Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Codon usage optimized for human usage

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW57.1 _3xHA-Ago2

    Plasmid

    #73538

    Purpose

    Inducible lentiviral expression of Ago2

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    Ago2 (Ago2 Mouse)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. TRE-UBC-hCD59-T2A-GFP

    Plasmid

    #205460

    Purpose

    lentiviral plasmid for expression of human CD59

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    hCD59 (CD59 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGAPTrap-mCherry-IRESMygro

    Plasmid

    #82505

    Purpose

    Vector targeting the mCherry gene to the GAPDH locus of human cells. mCherry is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    mCherry-IRESMygro
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGAPTrap-eGFP-IRESMygro

    Plasmid

    #82503

    Purpose

    Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    eGFP-IRESMygro
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. INS-GFP-CD19-EPT

    Plasmid

    #210469

    Purpose

    Donor plasmid for knock-in GFP-CD19 into the human INS locus

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    INS homologous recombination arms with a GFP-CD19 and EF1alpha-drived puromycin-NLS-tdTomato selection cassette (INS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCJ207

    Plasmid

    #162680

    Purpose

    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGAPTrap-eGFP-IRESMuro

    Plasmid

    #82504

    Purpose

    Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    eGFP-IRESMuro
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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