We narrowed to 10,893 results for: ENA;

1. pMOD_A0601

   Plasmid

   #91016

   Purpose

   Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Csy4-P2A-AtCas9_D10A nickase
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A

   Promoter

   35S

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJG363

   Plasmid

   #91183

   Purpose

   BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase (nAtCas9_D10A+gNt_R2+donor)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   nAtCas9_D10A+gNt_R2+donor
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJG370

   Plasmid

   #91187

   Purpose

   BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase no gRNA control (nAtCas9_D10A+gRNActrl+donor)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   nAtCas9_D10A+gRNActrl+donor
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Bongwoori

   Plasmid

   #63720

   Purpose

   Consists of the triple mutant voltage sensing domain and the optimized linker between the S4 transmembrane domain and the super ecliptic pHlorin A227D

   Depositor

   Bradley Baker

   Article

   Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

   Insert

   Bongwoori
   

   Use

   Tags

   Super ecliptic pHlorin A227D

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pQCi-hTRAC-sgRNA

   Plasmid

   #154093

   Purpose

   pQCi backbone with sgRNA targeting human TCR-alpha common chain

   Depositor

   Scott McComb

   Article

   Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

   Insert

   sgRNA targeting human TRAC locus
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. AA015

   Plasmid

   #216058

   Purpose

   Fragmid fragment: (2A Selection) Puromycin resistance, T2A, EGFP

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   P2A_v1.1; PuroR_v1.1; T2A_v1.2; EGFP_v1.1; Stop
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AA176

   Plasmid

   #216063

   Purpose

   Fragmid fragment: (2A Selection) mCherry

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   P2A_v1.1; mCherry_v1.1; Stop
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRVdG-4RFP

   Plasmid

   #52489

   Purpose

   Expresses TagRFP-T

   Depositor

   Sebastian Seung

   Article

   Nakashiba et al Cell. 2012 Mar 30;149(1):188-201. doi: 1

   Insert

   TagRFP-T
   

   Use

   Rabies virus

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYES260-sc.eEF2

   Plasmid

   #85119

   Purpose

   yeast expression of EEF2

   Depositor

   Pal Falnes

   Article

   Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

   Insert

   EFT1/EFT2 (EFT2, EFT1 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. RCAS (A) Wnt3a (CT#169)

    Plasmid

    #13976

    Depositor

    Cliff Tabin

    Article

    Kengaku et al Science. 1998 May 22. 280(5367):1274-7.

    Insert

    Wnt3a (Wnt3a Mouse)
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pLX208-gLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4

    Plasmid

    #125230

    Purpose

    For generating SPARK NanoLuc-oβ2AR transmembrane component lentivirus

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    gLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4
    

    Use

    Lentiviral

    Tags

    HA,Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJMP1217

    Plasmid

    #119258

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p2CT-His-MBP-Lbu_C2c2_R472A_H477A

    Plasmid

    #83483

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R472A_H477A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R472A, H477A

    Promoter

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. YEpURA-CUP1-HBTubiquitin

    Plasmid

    #26900

    Depositor

    Peter Kaiser

    Article

    Tagwerker et al Mol Cell Proteomics. 2006 Apr . 5(4):737

    Insert

    HBT-tagged ubiquitin
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. S12.1_Empty_Bridge

    Plasmid

    #127670

    Purpose

    Ampicillin resistant multiplexing backbone without CRY2HA and mCherry

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMOD_C2813

    Plasmid

    #91092

    Purpose

    Module C, Promoter: PvUbi2, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4 (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    PvUbi2

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSilencer2.1-U6-3JB1F+2

    Plasmid

    #190833

    Purpose

    For mammalian expression of 3JB1F+2, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.

    Depositor

    Gerald Hart

    Article

    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

    Insert

    3JB1F_T1+2bp (A Dual-Specificity aptamer targeting OGT and GFP)
    

    Use

    Affinity Reagent/ Antibody and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 9, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pK RagA(Q66L)-RagC

    Plasmid

    #99700

    Purpose

    Bacterial expression of RagA(Q66L)-RagC

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag, Poly Arg, and SUMO

    Expression

    Bacterial

    Mutation

    L91P

    Promoter

    T7

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJMP1069

    Plasmid

    #119244

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

    Plasmid

    #185687

    Purpose

    Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-GST-2xHaloTag-KKMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge