We narrowed to 13,780 results for: TIM

1. pUC-hU6-crScaffold_SV40-BFP

   Plasmid

   #224860

   Purpose

   crRNA scaffold for RfxCas13d expressed from hU6 promoter and reporter BFP protein expressed from SV40 promoter

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Orange Nano-lantern(Ca2+)/pcDNA3

   Plasmid

   #71346

   Purpose

   Express Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cell

   Depositor

   Takeharu Nagai

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Orange Nano-lantern(Ca2+)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pCNL-N1_vinculin

   Plasmid

   #65691

   Purpose

   Expresses Vinculin-CNL in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   vinculin (Vcl Mouse)
   

   Use

   Luciferase

   Tags

   CNL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

4. CRY2olig(FL)-mCherry

   Plasmid

   #60033

   Purpose

   Expresses a fusion of full length CRY2 (E490G) with mCherry

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

   Insert

   CRY2 E490G (deltaNLS) (CRY2 Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E490G, NLS mutated

   Promoter

   CMV

   Available since

   Oct. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET22b_EcSSB_1

   Plasmid

   #78203

   Purpose

   Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor

   Depositor

   Martin Webb

   Article

   Dillingham et al Biophys J. 2008 Oct;95(7):3330-9. doi: 1

   Insert

   ssb (G26C)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G26C (please see depositor comment below)

   Promoter

   T7

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJH4542

   Plasmid

   #200779

   Purpose

   Ptwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptune

   Depositor

   Mei Zhen

   Article

   Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

   Insert

   GCaMP6
   

   Use

   Tags

   mNeptune

   Expression

   Worm

   Mutation

   Promoter

   Ptwk-40

   Available since

   May 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pPL5618_pUG_MtxR

   Plasmid

   #60929

   Purpose

   PCR template vector for generating methotrexate resistance cassette.

   Depositor

   Robert Piper

   Article

   MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

   Insert

   Dihydrofolate reductase (DHFR Human)
   

   Use

   Loxp flanked deletion cassette template

   Tags

   Expression

   Mutation

   L22F F31S

   Promoter

   TEF1

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.2/NS3

   Plasmid

   #120822

   Purpose

   Ectopic expression of ZIKV protein

   Depositor

   Jan Rehwinkel

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   NS3 ZIKV
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYSD300

   Plasmid

   #212930

   Purpose

   Encodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   AGA1 (AGA1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.2/NS2B

    Plasmid

    #120819

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    NS2B ZIKV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.2/NS5-YFP

    Plasmid

    #120831

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    ZIKV NS5-eYFP
    

    Use

    Tags

    eYFP-V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMJA167

    Plasmid

    #128536

    Purpose

    Expresses DsRed

    Depositor

    Marcos Alcocer

    Article

    Wang et al Mol Biotechnol. 2014 Feb;56(2):136-46. d

    Insert

    NFAT-DsRed reporter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40-NFAT

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEB2-mNeptune2.5

    Plasmid

    #104013

    Purpose

    Plasmid encoding mNeptune2.5

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mNeptune2.5
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (MSKGEE LIKENM… M11…

    Promoter

    proC

    Available since

    Dec. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-CAG-Electra1-P2A-EGFP

    Plasmid

    #184935

    Purpose

    Expresses blue fluorescent protein Electra1 and EGFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Electra1-P2A-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAVS1_EF1a-MitoTrap-mCherry-IRES-Puro

    Plasmid

    #197067

    Purpose

    Donor plasmid for human AAVS1 locus knock-in, constitutive expression of MitoTrap-mCherry

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    MitoTrap-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUCHR-inmCherry

    Plasmid

    #60239

    Purpose

    HIV-1 transfer vector that encodes mcherry reporter gene interrupted by intron

    Depositor

    Dmitriy Mazurov

    Article

    Shunaeva et al J Virol. 2015 Oct 15;89(20):10591-601. d

    Insert

    inmCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ScFv-2ERT2-VPH

    Plasmid

    #120556

    Purpose

    Encode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to 2 tandem ERT2, VP64, P65 and HSF1

    Depositor

    Yu Wang

    Article

    Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

    Insert

    scFvGCN4, GB1, ERT2, VP64, P65 and HSF1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMU-4

    Plasmid

    #172353

    Purpose

    CRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNA

    Depositor

    Eriko Takano

    Article

    Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

    Insert

    D4-TheoR-cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    D4

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-FOXO3a WT-ER

    Plasmid

    #8355

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    FOXO3a (FOXO3 Human)
    

    Use

    Tags

    HA (YDVPDYASL)

    Expression

    Mammalian

    Mutation

    mouse estrogen receptor (G525R) on C-term

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMMK ENTR 2

    Plasmid

    #206261

    Purpose

    ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Type

    Empty backbone

    Use

    Multimate/gateway entr 2

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge