We narrowed to 17,706 results for: jun

1. 3xFLAG-dCas9/p-bacteria

   Plasmid

   #64325

   Purpose

   Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR; Tetracycline-inducible

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   pLtetO-1

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRK5-HA GST RagB wt

   Plasmid

   #19301

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   Ras-related GTP binding B (RRAGB Human)
   

   Tags

   GST and HA

   Expression

   Mammalian

   Available Since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRK5-HA GST RagA wt

   Plasmid

   #19298

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   Ras-related GTP binding A (RRAGA Human)
   

   Tags

   GST and HA

   Expression

   Mammalian

   Available Since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AW01_pU.CAG.eSpCas9(1.1).rBGpA

   Plasmid

   #199255

   Purpose

   Expression construct encoding the high specificity eSpCas9(1.1) nuclease

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5

   Insert

   High specificity eSpCas9(1.1) nuclease
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   K848A K1003A R1060A

   Promoter

   CAG promoter

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Map3k1

   Plasmid

   #29581

   Depositor

   Dustin Maly

   Article

   Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

   Insert

   Map3k1 (MAP3K1 Human)
   

   Tags

   GST, HIS6, and TEV

   Expression

   Bacterial

   Mutation

   Constitutively Active. Contains catalytic domain …

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

6. Rtn2b-mCherry

   Plasmid

   #186600

   Purpose

   Encodes human full-length Reticulon 2 isoform B fused to mCherry

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Human Reticulon 2 isoform B (RTN2 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CMV-Halo-10-PFN1

   Plasmid

   #204222

   Purpose

   Expression of Halo-Profilin-1 in tissue culture cells

   Depositor

   Jessica Henty-Ridilla

   Article

   Pimm et al Elife. 2022 Jun 6;11:e76485. doi: 10.755

   Insert

   PFN1 (PFN1 Human)
   

   Tags

   Halo, TEV site, SGLRSRAQAS linker

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC57 mini-ZsGreen

   Plasmid

   #222648

   Purpose

   Vector for cloning gene of interest for in vitro transcription

   Depositor

   Silvia Monticelli

   Article

   Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

   Insert

   ZsGreen
   

   Use

   In vitro transcription

   Mutation

   mutated stop codon

   Promoter

   T7

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRK5-HA GST RagB 54L

   Plasmid

   #19302

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   Ras-related GTP binding B (RRAGB Human)
   

   Tags

   GST and HA

   Expression

   Mammalian

   Mutation

   T54L

   Available Since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA5/FRT/TO_STf_LamB1

    Plasmid

    #181999

    Purpose

    CMV overexpression of SNAP-tag fast fusion to Lamin B1 for nuclear envelope localization in mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    STf-LamB1
    

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-PEmax-SpG-P2A-hMLH1dn

    Plasmid

    #211550

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

    Insert

    PEmax-SpG-P2A-hMLH1dn
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EasyFusion Halo-mAID

    Plasmid

    #112852

    Purpose

    General donor vector with Halo-mAID coding sequence flanking by restriction sites for generating knock-in donor for Auxin inducible degradation of Halo tagged endogenous protein

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Halo-mAID
    

    Use

    Synthetic Biology

    Promoter

    No

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFUGW-hGtACR1-EYFP

    Plasmid

    #67795

    Purpose

    Mammalian expression construct of human codon-adapted anion channelrhodopsin 1 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone

    Depositor

    John Spudich

    Article

    Govorunova et al Science. 2015 Jun 25. pii: aaa7484.

    Insert

    hGtACR1
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQdCas9-sgempty

    Plasmid

    #159523

    Purpose

    Expression of dCas9 driven by lux cassette from Vibrio fischeri

    Depositor

    Radhakrishnan Mahadevan

    Article

    Liu et al ACS Synth Biol. 2020 Jun 5. doi: 10.1021

    Insert

    quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dcas9 and sgRNA
    

    Use

    CRISPR

    Promoter

    quorum sensing promoter

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TRPML3-YFP

    Plasmid

    #18835

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML3 (Mcoln3 Mouse)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Q165R, F210Y

    Available Since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT7-EGFP-C1-HsTNRC6A

    Plasmid

    #25035

    Depositor

    Elisa Izaurralde

    Article

    Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO.1 Rheb1 shRNA #1

    Plasmid

    #26625

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Rheb1 shRNA (RHEB Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pRK5-HA GST RagD 77L

    Plasmid

    #19308

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Ras-related GTP binding D (RRAGD Human)
    

    Tags

    GST and HA

    Expression

    Mammalian

    Mutation

    S77L

    Available Since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCW57.1-mDux-CA

    Plasmid

    #99284

    Purpose

    All-in-one lentivector with tet-inducible mouse Dux (codon sequences altered)

    Depositor

    Stephen Tapscott

    Article

    Whiddon et al Nat Genet. 2017 Jun;49(6):935-940. doi:

    Insert

    Dux-CA (mouse; codon altered) (Dux Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5-HA GST RagD wt

    Plasmid

    #19307

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Ras-related GTP binding D (RRAGD Human)
    

    Tags

    GST and HA

    Expression

    Mammalian

    Available Since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge