We narrowed to 15,985 results for: nes
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Plasmid#14686DepositorAvailable SinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only
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pCMV-hA3G-BE3
Plasmid#113415PurposeExpresses hA3G-BE3 in mammalian cellsDepositorInserthA3G-BE3 (APOBEC3G S. pyogenes and Bacteriophage PBS2, Human)
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-human NMyc-humanER-IRES-mCherry
Plasmid#73571PurposeMammalian retroviral expression of NMyc-ERDepositorInsertNMyc (MYCN Human)
UseRetroviralTagsHuman ER and IRES-mCherryExpressionMammalianPromoterMSCVAvailable SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn
Plasmid#214102PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dnDepositorInsertPE-Plus
ExpressionMammalianAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACM4-ACA
Plasmid#49809PurposeEncodes fabD, accA, accB, accC, accD in pseudo-operon configuration.DepositorInsertsACP transacylase
Acetyl-CoA carboxylase
acetyl-CoA carboxylase
Acetyl-CoA carboxylase
Acetyl-CoA carboxylase
ExpressionBacterialMutationSilent site-directed mutagenesis to remove SalI s…PromoterT7Available SinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPHAGE-CR-PINK1-C-TAP
Plasmid#128510PurposeLentiviral constitutive expression of CRISPR-resistant PINK1 with c-terminal FLAG and HA tag.DepositorInsertPTEN induced kinase 1 (PINK1 Human)
UseLentiviralTagsFLAG and HA tagsExpressionMammalianMutationSynonymous silent mutation in Q5 to render sgRNA …PromoterCMVAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-EglN3 H196A-pcDNA3
Plasmid#22717DepositorInsertEgg laying Nine - 3 (EGLN3 Human)
TagsHA-tagExpressionMammalianMutationmutant cDNA: point mutation at codon 196 was ins…Available SinceDec. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag LIMK2
Plasmid#20513DepositorAvailable SinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α P405A/P531A/N847A-pBabe-Puro
Plasmid#25956DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…Available SinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PAK4
Plasmid#20460DepositorAvailable SinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV TRE3G Neo GFP-Progerin C661S
Plasmid#118711PurposeLentiviral TET-ON inducible GFP-Progerin C661S (Farnesylation mutant)DepositorInsertGFP-Progerin C661S (LMNA Human)
UseLentiviral; Tet-on inducibleTagsGFPExpressionMammalianMutationC661S farnesylation mutantPromoterCMV TRE3G (TET-ON)Available SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag MAPK13
Plasmid#20523DepositorAvailable SinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag YES1
Plasmid#20665DepositorAvailable SinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag AKAP8L
Plasmid#20481DepositorAvailable SinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s-GFP
Plasmid#66083PurposeG protein alpha-s internally tagged with EGFP and EE epitopeDepositorInsertG-alpha-s-EE-EGFP (Gnas Rat)
TagsEGFP flanked by SGGGS on each side was inserted i…ExpressionMammalianMutationBamHI sites in the alpha-s cDNA were removed and …PromoterCMVAvailable SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin
Plasmid#99277PurposeDual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistanceDepositorInsertsH2B-mOrange2-IRES-EGFP
Puro
UseLentiviralTagsEGFP, H2B, IRES, PGK, and mOrange2PromoterEF1 alpha-human and PGK-mouseAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-racRNA-B
Plasmid#200825PurposeAAV transfer plasmid encoding a circularized RNA barcode B under U6 promoter and a subcellular localization protein binder under hSyn promoterDepositorInsertsU6-racRNA
V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
UseAAVTagsC-Far, Flag, and V5ExpressionMammalianPromoterhSynAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-racRNA-C
Plasmid#200826PurposeAAV transfer plasmid encoding a circularized RNA barcode C under U6 promoter and a subcellular localization protein binder under hSyn promoterDepositorInsertsU6-racRNA
V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
UseAAVTagsC-Far, Flag, and V5ExpressionMammalianPromoterhSynAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-racRNA-D
Plasmid#200827PurposeAAV transfer plasmid encoding a circularized RNA barcode D under U6 promoter and a subcellular localization protein binder under hSyn promoterDepositorInsertsU6-racRNA
V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
UseAAVTagsC-Far, Flag, and V5ExpressionMammalianPromoterhSynAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TRS-Leader-SARS-CoV-2-EGFP
Plasmid#171585PurposeExpression of 75 nt of the SARS-CoV-2 Leader sequence at 5' of destabilized eGFP reporterDepositorInsertTRS-Leader-SARS-CoV-2-d2eGFP
UseLentiviralTags2PEST AND NLSAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only