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pESC-MET

Plasmid

#222482

Purpose

Co-expression of two genes in yeast with FLAG and MYC tags and MET15 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

FLAG and Myc

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pESC-LYS

Plasmid

#222483

Purpose

Co-expression of two genes in yeast with FLAG and MYC tags and LYS2 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

FLAG and Myc

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-eDHFR-NAT

Plasmid

#188926

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-NAT

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBEVY-GU

Plasmid

#51229

Purpose

bi-directional expression vector for yeast, galactose inducible, URA3 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GAL1,10

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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FRP718_PACT1(-1-520)-LexA-ER-haB42-TCYC1

Plasmid

#58431

Purpose

Expresses LexA-ER-B42 in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-B42

Expression

Yeast

Promoter

Act1 promoter (-520;1)

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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N114 nLV EF-1a-Gal4-VP16-PGK-Puro

Plasmid

#44014

Depositor

Jerry Crabtree

Article

Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

Insert

Gal4/DBD (1-147)

Use

Lentiviral

Tags

VP16/Activation/Domain

Promoter

EF-1a

Available Since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

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pBEVY-GL

Plasmid

#51225

Purpose

bi-directional expression vector for yeast, galactose inducible, LEU2 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GAL1,10

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pCCL-GKL1RC-Short-LEUg1mKateg2GKL2ORF10UCS

Plasmid

#52345

Purpose

Integration cassette to create p1-Short-Leu-mKate with p2 ORF10 UCS>mKate

Depositor

Chang Liu

Article

Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

Insert

mKate2

Use

P1 integration vector

Expression

Bacterial

Mutation

changed V2H

Promoter

pGKL2 ORF10 UCS

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pRN1120

Plasmid

#101750

Purpose

Multi-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.

Depositor

Rene Verwaal

Article

Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

Type

Empty backbone

Expression

Yeast

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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YIplac211-SEC7-mCherry2Bx6

Plasmid

#105267

Purpose

Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-mCherry2Bx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Sec7 portion (SEC7 Budding Yeast)

Tags

mCherry2Bx6

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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FRP467-PACT1(-1-520)-LexA-ER-haVP16

Plasmid

#58430

Purpose

Expresses LexA-ER-VP16 in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-VP16

Expression

Yeast

Promoter

Act1 promoter (-520;1)

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPH1-mEGFP

Plasmid

#105270

Purpose

Plasmid for pop-in/pop-out replacement of Vph1 with Vph1-mEGFP.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vph1 portion (VPH1 Budding Yeast)

Tags

mEGFP

Expression

Yeast

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pTH650-RLuc/staFLuc

Plasmid

#29696

Depositor

Tobias von der Haar

Article

Chu et al Nucleic Acids Res. 2011 Aug;39(15):6705-

Insert

Firefly Luciferase

Expression

Yeast

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only

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YIplac211-SEC7-msCFPx6

Plasmid

#105268

Purpose

Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-msCFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Sec7 portion (SEC7 Budding Yeast)

Tags

msCFPx6

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-HALO-URA

Plasmid

#188932

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-HALO-URA

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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FRP991_PACT1(-1-520)-LexA-ER-Gal4AD

Plasmid

#58438

Purpose

Expresses LexA-ER-Gal4AD from yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-Gal4AD

Expression

Yeast

Promoter

Act1 promoter (-520;1)

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pBPGw

Plasmid

#17574

Depositor

Gerald Rubin

Article

Pfeiffer et al Tools for neuroanatomy and neurogenetics

Insert

GAL4 (GAL4 Budding Yeast)

Expression

Insect

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only
pBPHLWL-VP16AD

Plasmid

#26259

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

VP16AD-ZIP+

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fSNAP-Hygromycin

Plasmid

#188914

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fSNAP-Hygromycin

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pWS931

Plasmid

#123053

Purpose

TetR bacterial repressor

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

TetR-NLS [3a]

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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