We narrowed to 18,356 results for: Tec

1. pLR5-CAG-NLS-tdiRFP670

   Plasmid

   #82621

   Purpose

   Expresses NLS-tdiRFP670 in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

   Insert

   NLS-tdiRFP670
   

   Use

   Piggybac vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pML2003

   Plasmid

   #193255

   Purpose

   Propionate sensor expressing eYFP reporter

   Depositor

   Lauren Andrews

   Article

   Lebovich et al Front Bioeng Biotechnol. 2022 Aug 25;10:

   Inserts

   prpR
   eYFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Pprp and Synthetic promoter P6

   Available since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA4/TO-2xStrep-BFLF1

   Plasmid

   #162626

   Purpose

   Expresses N-terminal 2x-Strep-tagged BFLF1 in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   BFLF1
   

   Use

   Tags

   N-terminal 2xStrep-Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. mEos2-VASP-5

   Plasmid

   #57433

   Purpose

   Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

   Insert

   VASP
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   A209T in VASP

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pTMS2

   Plasmid

   #87800

   Purpose

   For constructing MBP-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.

   Depositor

   Yu-Zhu Zhang

   Article

   Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

   Type

   Empty backbone

   Use

   Tags

   6xHis and Strep II

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-IL2sp-6His-Asprosin plasmid

   Plasmid

   #190683

   Purpose

   Expresses His-tagged asprosin in mammalian cells. An IL2 signal peptide at N-terminus allows secretion of asprosin from the transduced cells.

   Depositor

   Atul Chopra

   Article

   Mishra et al STAR Protoc. 2022 Dec 16;3(4):101762. do

   Insert

   IL2-6xHis-Asprosin
   

   Use

   Tags

   IL2 signal peptide, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. GGE209

   Plasmid

   #120793

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsUp-Mfe
   

   Use

   Cloning/assembly plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCfB5935

   Plasmid

   #106128

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntA_1, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. myc-Rapr-FAK-YM

   Plasmid

   #25927

   Depositor

   Klaus Hahn

   Article

   Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

   Insert

   RapR-FAK (Ptk2 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   This myc-RapR-FAK-YM plasmid contains FAK mutatio…

   Promoter

   Available since

   Aug. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pX330A_FokI-1x4

    Plasmid

    #63604

    Purpose

    Expresses FokI-dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    FokI-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. TAL2368

    Plasmid

    #36779

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    NCOR1-TALEN_Left (Ncor1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pHCKan-yibDp-mCherry

    Plasmid

    #127058

    Purpose

    Low phosphate inducible mCherry with N-terminal linker and His tag

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. BCJJ340

    Plasmid

    #117502

    Purpose

    UBI10:Cas9:E9 Golden Gate Level 1 Position 2

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUBI10

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. YSBE 558 : pNAT_4xFLAG

    Plasmid

    #92086

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; 4xFLAG tag, NAT selection

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    4xFlag
    

    Use

    tagging proteins in cryptococcus neoforman

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFLIP37

    Plasmid

    #65505

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-Cit-attR1 and attR2-Cer-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCellFree_G03 SREBF2

    Plasmid

    #67129

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    SREBF2 (SREBF2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pCfB2192

    Plasmid

    #67539

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae XII-2 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. YSBE 628 : pHYG_mCherry

    Plasmid

    #92092

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; mCherry tag, HYG selection

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    mCherry
    

    Use

    tagging proteins in cryptococcus neoforman

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mApple-VASP-5

    Plasmid

    #54958

    Purpose

    Localization: Actin/Focal Adhesions, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    VASP
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    A209T in VASP

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pCoofy47

    Plasmid

    #55188

    Purpose

    Mammalian expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    10x His-Stop-OneStrep-Stop-S-tag-Stop-CBP-Stop-HP…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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