3,636 results

1. pTIGER_tdTomato::3xFLAG::dCrk

   Plasmid

   #131138

   Purpose

   UASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   Crk (Crk Fly)
   

   Use

   Tags

   3XFLAG and tdTomato

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRD119 (PCNA GFP)

   Plasmid

   #14822

   Depositor

   Robert Duronio

   Article

   Thacker et al Curr Biol. 2003 Jan 8. 13(1):53-8.

   Insert

   PCNA-GFP (PCNA Fly)
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pCFJ1202

   Plasmid

   #44484

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Insert

   Minimal Mos1, NeoR, peel-1, MCS
   

   Use

   Multiple cloning site vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUAST-SIK3 WT

   Plasmid

   #52972

   Purpose

   insect expression of SIK3 WT

   Depositor

   Marc Montminy, Biao Wang

   Article

   Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

   Insert

   SIK3 (Sik3 Fly)
   

   Use

   Tags

   HA

   Expression

   Insect

   Mutation

   Promoter

   Hsp70

   Available since

   May 5, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pAc-sgRNA-Cas9.scr_2

   Plasmid

   #190704

   Purpose

   Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Scrambled sgRNA 2
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Drosophila U6

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 20XUAS IVS Jaws mCherry_tr

   Plasmid

   #111549

   Purpose

   Optogenetics in Drosophila: UAS-induced expression of Jaws mCherry

   Depositor

   Vivek Jayaraman

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws_mCherry_trafficked
   

   Use

   Tags

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-calx1

   Plasmid

   #58697

   Purpose

   Expression of calx1

   Depositor

   Craig Montell

   Article

   Wang et al Neuron 2005 Feb 3;45(3):367-78.

   Insert

   calx1 (Calx Fly)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2014

   Availability

   Academic Institutions and Nonprofits only

8. orthodenticle

   Plasmid

   #37285

   Depositor

   Norbert Perrimon

   Article

   Finkelstein et al Genes Dev. 1990 Sep;4(9):1516-27.

   Insert

   orthodenticle (oc Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pFBCPDL6

   Plasmid

   #67285

   Purpose

   CPD photolyase from Drosophila with Flag tag in pFastbac vector. Intermediate in insect cell expression

   Depositor

   Aziz Sancar

   Article

   Selby et al Biochemistry. 2012 Jan 10;51(1):167-71.

   Insert

   CPD photolyase (phr Fly)
   

   Use

   Bacterial vector, insert not expressed

   Tags

   Flag

   Expression

   Mutation

   V275E*

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

10. KHBD00332

    Plasmid

    #34455

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG1765 (EcR Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pBSc polyA + Shal1

    Plasmid

    #16196

    Depositor

    Larry Salkoff

    Article

    Wei et al Science. 1990 May 4. 248(4955):599-603.

    Insert

    Drosophila melanogaster Shaker cognate l (Shal Fly)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pUAST-Myc-dATG1

    Plasmid

    #111801

    Purpose

    Insect Expression of dAtg1

    Depositor

    Jun Hee Lee

    Article

    Kim et al Autophagy. 2013 Aug;9(8):1201-13. doi: 1

    Insert

    dAtg1 (Atg1 Fly)
    

    Use

    Tags

    Myc

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJFRC210-10XUAS-FRT>STOP>FRT-myr::smGFP-OLLAS

    Plasmid

    #63170

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-OLLAS reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-OLLAS"
    

    Use

    Tags

    N-myristoylation

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEM-T Dmel Or67b

    Plasmid

    #59672

    Purpose

    Probe for Drosophila melanogaster Or67b expression

    Depositor

    Leslie Vosshall, Richard Axel

    Article

    Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

    Insert

    Or67b (Or67b Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pMT-Pav-GFP

    Plasmid

    #24286

    Depositor

    Ron Vale

    Article

    Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

    Insert

    Pavarotti (pav Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

16. 1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]

    Plasmid

    #149426

    Purpose

    The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Inserts

    U6.3-gRNAs[Sxl, bTub]
    Hsp70Bb-Cas9-T2A-eGFP-p10
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pZG71

    Plasmid

    #202577

    Purpose

    RNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-dADARcd-35S-hptII

    Depositor

    Guoyong Xu

    Article

    Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

    Insert

    HyperADARcd
    

    Use

    Tags

    Expression

    Plant

    Mutation

    E488Q

    Promoter

    Available since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUASP dsRed Rab27 WT

    Plasmid

    #44832

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab27 (Rab27 Fly)
    

    Use

    Drosophila

    Tags

    dsRed

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pUAST-SIK3 T196A

    Plasmid

    #52974

    Purpose

    insect expression of SIK3 T196A (LKB1 phosphorylation defective mutant)

    Depositor

    Marc Montminy, Biao Wang

    Article

    Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

    Insert

    SIK3 (Sik3 Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    T196A LKB1 phosphorylation defective mutant

    Promoter

    Hsp70

    Available since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pUAST-SIK3 K70M

    Plasmid

    #52973

    Purpose

    insect expression of SIK3 K70M (kinase dead mutant)

    Depositor

    Marc Montminy, Biao Wang

    Article

    Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

    Insert

    SIK3 (Sik3 Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    K70M kinase dead mutant

    Promoter

    Hsp70

    Available since

    May 5, 2014

    Availability

    Academic Institutions and Nonprofits only