Addgene

We narrowed to 880 results for: fkbp

Showing: 481 - 510 of 880 results

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PC3-709-33-10_GAL4-FKBP12(F36V)

Plasmid

#200438

Purpose

Expresses the GAL4 DNA Binding domain linked to the FKBP12(F36V) target protein

Depositor

Xue Gao

Article

Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1

Insert

GAL4-FKBP12(F36V)

Use

Tags

GAL4, FKBP12(F36V)

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe NGF-ERBB2-2xFKBP-HA hygro

Plasmid

#192292

Purpose

Retroviral expression vector for NGF-ERBB2 -2xFKBP-HA construct

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

NGF-ERBB2-2xFKBP-HA (ERBB2 Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe NGF-Erbb2-2xFKBP-HA hygro

Plasmid

#192291

Purpose

Retroviral expression vector for NGF-Erbb2-2xFKBP-HA construct

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

NGF-Erbb2-2xFKBP-HA (Erbb2 Rat)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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Phage-PGK-Flag-HA-FKBP-T(G177D)

Plasmid

#181735

Purpose

for lentiviral expression of brachyury ORF in mammalian cells

Depositor

Charles Lin

Article

Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

Insert

T (brachyury)

Use

Lentiviral

Tags

Expression

Mutation

a silent mutation in the PAM site of an sgRNAt ta…

Promoter

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pT2Aneo-mRFP-FKBP-mSos1-linkercat

Plasmid

#173858

Purpose

Encoding a part of the rapamycin-induced Ras activation system.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

mRFP-FKBP-mSos1-linkercat

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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PB-PGK-FKBP-Zte-Cit-tv-DHFR

Plasmid

#116049

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

FKBP-Zte-Cit-tv-DHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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PB-PGK-FKBP-Zte-Cit-hc-DHFR

Plasmid

#116047

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

FKBP-Zte-Cit-hc-DHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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PB-CMV-FKBP-Zte-Cit-hc-DHFR

Plasmid

#116059

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

FKBP-Zte-Cit-hc-DHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-FKBP12_F36V-CasRx-tagBFP-PGK-Blasticidin

Plasmid

#187952

Purpose

FKBP12 (F36V mutant) degron-tagged CasRx fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

FKBP12_F36V-CasRx-tagBFP

Use

CRISPR and Synthetic Biology

Tags

GGS linker, HA-tag, and NLS

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLX304 CMV FKBP-V5-sTurboID-high affinity (N)

Plasmid

#153004

Purpose

Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID-high affinity (N) (G78)

Use

Lentiviral

Tags

FKBP and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+) miniTurboID-6xGGSG linker-FKBPF36V-2xHA

Plasmid

#200641

Purpose

Expresses miniTurboID-FKBPF36V-2xHA in mammalian cells.

Depositor

Fleur Ferguson, Justin English

Article

Tao et al Nat Commun. 2023 Dec 4;14(1):8016. doi:

Insert

miniTurboID-6xGGSG linker-FKBPF36V-2xHA

Use

Tags

HA-tag

Expression

Mammalian

Mutation

Promoter

T7

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-mEGFP-WIPI2d (R108E/R125E)

Plasmid

#223770

Purpose

Stable expression and inducible recruitment of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

WIPI2d (WIPI2 Human)

Use

Lentiviral

Tags

FKBP-mEGFP

Expression

Mutation

R108E/R125E

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti PGK FKBP12(F36V)-OGT (Neo)

Plasmid

#154294

Purpose

Lentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoter

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Lentiviral

Tags

2x HA and FKBP12(F36V)

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir

Plasmid

#90009

Purpose

Split-BioID plasmid: Expresses C-terminally tagged CBir[257-321]-FRB and C-terminally tagged NBir[2-256]-FKBP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

FKBP-linker-Myc-NBirA*
FRB-linker-CBirA*

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII(0.3)-mVenus-FKBP-K7GEF

Plasmid

#225828

Purpose

A component of SYNCit-K (Synapse-targeted chemically induced translocation of Kalirin-7)

Depositor

Haruo Kasai

Article

Sawada et al Science. 2024 Sep 27;385(6716):1459-1465

Insert

mVenus-FKBP-K7GEF

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-CBir-FRB_Myc-NBir-FKBP

Plasmid

#90003

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and N-terminally tagged NBir[2-256]-FKBP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

NBirA*-linker-FKBP
CBirA*-linker-FRB

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP

Plasmid

#90008

Purpose

Split-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

NBirA*-linker-FKBP
FRB-linker-CBirA*

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-CBir-FRB_FKBP-Myc-Nbir

Plasmid

#90004

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and C-terminally tagged FKBP-NBir[2-256]

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

FKBP-linker-Myc-NBirA*
CBirA*-linker-FRB

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pBa-tdTomato-flag-BicD2 594-FKBP

Plasmid

#64205

Purpose

Bicaudal D protein aa1-594 (excluding start Met) with N-terminal tandem dimer Tomato and flag tags and C-terminal FKBP

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Bicaudal D protein (Bicd2 Mouse)

Use

Tags

FKBP, flag, and tdTomato

Expression

Mammalian

Mutation

no start methionine, aa1-594

Promoter

chicken Beta Actin

Available since

July 22, 2015

Availability

Academic Institutions and Nonprofits only

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pAAVS1_EF1a-GEM2-Halo-FKBP-IRES-Blast

Plasmid

#197066

Purpose

Donor plasmid for human AAVS1 locus knock-in, constitutive expression of GEM2-Halo-FKBP

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

Encapsulin

Use

CRISPR

Tags

FKBP and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBa-eGFP-flag-BicD2 594-FKBP

Plasmid

#63569

Purpose

Bicaudal D protein aa1-594 (excluding start Met) tagged with N-terminal eGFP and flag and a C-terminal FKBP

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Bicaudal D protein (Bicd2 Mouse)

Use

Tags

FKBP, eGFP, and flag

Expression

Mammalian

Mutation

no start methionine, aa1-594

Promoter

chicken Beta Actin

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FKBP-C-mSOG-J-alpha-IRES-mCherry

Plasmid

#136603

Purpose

Encodes FKBP tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).

Depositor

John Ngo

Article

Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

Insert

FKBP-C-mSOG-J-alpha

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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mCh-LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)

Plasmid

#165030

Purpose

Express cpFRB2-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

Depositor

Yubin Zhou

Article

Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

Insert

LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)

Use

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

T7

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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N118 nLV EF-1a-Gal-FKBPx1-HA-PGK-Blast

Plasmid

#44245

Depositor

Jerry Crabtree

Article

Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

Insert

FKBP (FKBP1A Human)

Use

Lentiviral

Tags

Gal4/DBD/aa1-147 and HA

Expression

Mutation

Promoter

EF-1a

Available since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

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pJB001 CMV 3xFLAG-NLS-FKBP-ZF2

Plasmid

#161545

Purpose

Constitutive expression of 3xFLAG-NLS-FKBP-ZF2 under the CMV promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-FKBP-ZF2

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSD36_pCAG-PV1-FKBP-L1-TP901-327C-BGHpA

Plasmid

#108814

Purpose

Expresses aa327 to C-terminus of TP901 integrase fused to FKBP

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

TP901 integrase fragment aa327 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pBW1309_pCAG-PV1-FKBP-L1-CTD-ENTRY-BGHpA

Plasmid

#108837

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to FKBP tag on N-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

FKBP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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PB-PGK-FKBP-Ztetv-Cit-tvhc-DHFR

Plasmid

#116052

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

FKBP-Ztetv-Cit-tvhc-DHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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FKBP-beta2(616-951)-GFP Double

Plasmid

#100729

Purpose

To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Use

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Long isoform, deletion of clathrin box motif and …

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pFLinkC-AI(GPG)-FKBP12(P9)-FRB(GPG)-TVMV

Plasmid

#137118

Purpose

Protease Switch Clone ID: 2-B3

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

AI(GPG)-FKBP12(P9)-FRB(GPG)-TVMV

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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