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Showing: 641 - 680 of 3556 results

  9. pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394

    Plasmid
    #128386
    Purpose
    Expresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruption
    Depositor
    Craig Duvall
    Article
    Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acss
    Inserts
    NLuc-3xGGGGS-Gal8-NCRD (LGALS8 Human, Synthetic)
    Gal8-NCRD-3xGGGGS-CLuc398 (LGALS8 Human)
    Use
    Lentiviral, Luciferase, and Synthetic Biology
    Tags
    C-terminal luc2 fragment and N-terminal luc2 frag…
    Expression
    Mammalian
    Mutation
    N terminal carbohydrate recognition domain fused …
    Promoter
    EF1A and IRES
    Available since
    Jan. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    128386-plasmid-map-sequence-id-263327

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  11. RIP7-RLuc-YFP-pBS

    Plasmid
    #72482
    Purpose
    Expresses a renilla luciferase-YFP fusion protein under control of the rat insulin 2 gene promoter (RIP7) suitable for transfection or generation of transgenic animals
    Depositor
    Vincent Poitout
    Article
    Ghislain et al Endocrinology. 2015 Dec 15:en20151550.
    Inserts
    rat ins2 gene promoter (Ins2 Rat)
    luciferase
    eYFP
    Use
    Luciferase
    Tags
    eYFP and renilla luciferase
    Expression
    Mammalian
    Mutation
    eYFP varient
    Promoter
    Available since
    March 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    72482-plasmid-map-sequence-id-136970

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  12. pLuc-Optop38i-lsm

    Plasmid
    #89747
    Purpose
    Expression of light-regulated p38 inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor MK3BD3-13F
    Depositor
    Michael Courtney
    Article
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    Optop38i lit-state mutant
    Use
    Luciferase
    Tags
    Firefly luciferase
    Expression
    Mammalian
    Mutation
    I539E mutation of AsLov2Jalpha
    Promoter
    CMV
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89747-plasmid-map-sequence-id-192491

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  13. pLuc-OptoJNKi-lsm

    Plasmid
    #89746
    Purpose
    Expression of light-regulated JNK inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11
    Depositor
    Michael Courtney
    Article
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    OptoJNKi lit-state mutant
    Use
    Luciferase
    Tags
    Firefly luciferase
    Expression
    Mammalian
    Mutation
    I539E mutation of AsLov2Jalpha
    Promoter
    CMV
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89746-plasmid-map-sequence-id-192490

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  15. pLuc-OptoJNKi-dsm

    Plasmid
    #89745
    Purpose
    Expression of light-regulated JNK inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11
    Depositor
    Michael Courtney
    Article
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    OptoJNKi dark-state mutant
    Use
    Luciferase
    Tags
    Firefly luciferase
    Expression
    Mammalian
    Mutation
    C450A mutation of AsLov2Jalpha
    Promoter
    CMV
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89745-plasmid-map-sequence-id-192487

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  16. psiCHECK2-miR-15-5p

    Plasmid
    #135709
    Purpose
    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-15-5p)
    Depositor
    Florian Karreth
    Insert
    miR-15-5p reverse complement
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135709-plasmid-map-sequence-id-322208

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  17. psiCHECK2-miR-145-5p

    Plasmid
    #135718
    Purpose
    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-145-5p)
    Depositor
    Florian Karreth
    Insert
    miR-145-5p reverse complement
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    135718-plasmid-map-sequence-id-322199

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  18. psiCHECK2-miR-106b-5p

    Plasmid
    #135713
    Purpose
    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-106-5p)
    Depositor
    Florian Karreth
    Insert
    miR-106b-5p reverse complement
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    135713-plasmid-map-sequence-id-322204

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  19. psiCHECK2-miR-142-3p

    Plasmid
    #135716
    Purpose
    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-142-3p)
    Depositor
    Florian Karreth
    Insert
    miR-142-3p reverse complement
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    135716-plasmid-map-sequence-id-322201

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  20. psiCHECK2-miR-96-5p

    Plasmid
    #135712
    Purpose
    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-96-5p)
    Depositor
    Florian Karreth
    Insert
    miR-96-5p reverse complement
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    135712-plasmid-map-sequence-id-322205

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  21. psiCHECK2-miR-144-3p

    Plasmid
    #135717
    Purpose
    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-144-3p)
    Depositor
    Florian Karreth
    Insert
    miR-144-3p reverse complement
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    135717-plasmid-map-sequence-id-322200

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  22. psiCHECK2-miR-19-3p

    Plasmid
    #135710
    Purpose
    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-19-3p)
    Depositor
    Florian Karreth
    Insert
    miR-19-3p reverse complement
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    135710-plasmid-map-sequence-id-322207

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  23. psiCHECK2-miR-130a-3p

    Plasmid
    #135714
    Purpose
    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-130a-3p)
    Depositor
    Florian Karreth
    Insert
    miR-130-3p reverse complement
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    135714-plasmid-map-sequence-id-322203

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  24. pLSU1/t35s[TMV]:nnLuz:NosT

    Plasmid
    #212188
    Purpose
    This binary vector expresses mushroom luciferase (nnLuz) with the truncated 35sCAMV promoter and Tomato mosaic virus UTR
    Depositor
    Wayne Curtis
    Insert
    nnLuz
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    truncated 35sCaMV
    Available since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212188-plasmid-map-sequence-id-422977

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  25. pLSU1/o35s[TMV]:nnLuz:NosT

    Plasmid
    #212189
    Purpose
    This binary vector expresses mushroom luciferase (nnLuz) with the original 35sCAMV promoter and Tomato mosaic virus UTR
    Depositor
    Wayne Curtis
    Insert
    nnLuz
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    original 35sCaMV
    Available since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212189-plasmid-map-sequence-id-422982

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  26. psiCHECK2-miR-16-5p

    Plasmid
    #135708
    Purpose
    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-16-5p)
    Depositor
    Florian Karreth
    Insert
    miR-16-5p reverse complement
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135708-plasmid-map-sequence-id-322209

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  27. psiCHECK2-miR-223-3p

    Plasmid
    #135723
    Purpose
    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-223-3p)
    Depositor
    Florian Karreth
    Insert
    miR-223-3p reverse complement
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135723-plasmid-map-sequence-id-322175

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  28. psiCHECK2-miR-199a-5p

    Plasmid
    #135721
    Purpose
    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-199a-5p)
    Depositor
    Florian Karreth
    Insert
    miR-199a-5p reverse complement
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    135721-plasmid-map-sequence-id-322177

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  29. psiCHECK2-miR-203a-3p

    Plasmid
    #135722
    Purpose
    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-203a-3p)
    Depositor
    Florian Karreth
    Insert
    miR-203a-3p reverse complement
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    135722-plasmid-map-sequence-id-322176

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  30. psiCHECK2-miR-489-3p

    Plasmid
    #135724
    Purpose
    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-489-3p)
    Depositor
    Florian Karreth
    Insert
    miR-489-3p reverse complement
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    135724-plasmid-map-sequence-id-322174

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  31. psiCHECK2-miR-503-5p

    Plasmid
    #135725
    Purpose
    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-503-5p)
    Depositor
    Florian Karreth
    Insert
    miR-503-5p reverse complement
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    135725-plasmid-map-sequence-id-322172

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  32. psiCHECK2-miR-23a-3p

    Plasmid
    #135711
    Purpose
    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-23a-3p)
    Depositor
    Florian Karreth
    Insert
    miR-23-3p reverse complement
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    135711-plasmid-map-sequence-id-322206

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  33. psiCHECK2-miR-183-5p

    Plasmid
    #135719
    Purpose
    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-183-5p)
    Depositor
    Florian Karreth
    Insert
    miR-183-5p reverse complement
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    135719-plasmid-map-sequence-id-322198

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  34. psiCHECK2-miR-194-5p

    Plasmid
    #135720
    Purpose
    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-194-5p)
    Depositor
    Florian Karreth
    Insert
    miR-194-5p reverse complement
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    135720-plasmid-map-sequence-id-322197

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  36. psiCHECK2-let-7 MT

    Plasmid
    #78261
    Purpose
    psiCHECK2 dual luciferase reporter harboring a mutated let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene
    Depositor
    Joanne Weidhaas
    Article
    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10
    Insert
    mutated let-7 target site
    Use
    Luciferase; Microrna activity
    Tags
    Expression
    Mammalian
    Mutation
    nucleotides 2-5 and 10-11 changed from CTCC to GA…
    Promoter
    Available since
    June 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    78261-plasmid-map-sequence-id-149478

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  37. AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-AAVS1

    Plasmid
    #184397
    Purpose
    Donor plasmid for stable integration of firefly luciferase NMD(+) PTC39 reporter at AAVS1
    Depositor
    Robert Bradley
    Article
    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:
    Insert
    Firefly luciferase beta-globin-PTC39
    Use
    CRISPR; Donor plasmid for hdr
    Tags
    3X FLAG
    Expression
    Mammalian
    Mutation
    PTC in beta-globin at amino acid position 39
    Promoter
    Tet-On 3G
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    184397-plasmid-map-sequence-id-362358

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  38. AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-AAVS1

    Plasmid
    #184398
    Purpose
    Donor plasmid for stable integration of Renilla luciferase NMD(+) PTC39 reporter at AAVS1
    Depositor
    Robert Bradley
    Article
    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:
    Insert
    Renilla luciferase beta-globin-PTC39
    Use
    CRISPR; Donor plasmid for hdr
    Tags
    3X FLAG
    Expression
    Mammalian
    Mutation
    PTC in beta-globin at amino acid position 39
    Promoter
    Tet-On 3G
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    184398-plasmid-map-sequence-id-362369

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  40. psiCHECK2-miR-34 MT

    Plasmid
    #78259
    Purpose
    psiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene
    Depositor
    Joanne Weidhaas
    Article
    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10
    Insert
    mutated miR-34 target site
    Use
    Luciferase; Microrna activity
    Tags
    Expression
    Mammalian
    Mutation
    nucleotides 2-5 and 10-11 changed from CCGT to GG…
    Promoter
    Available since
    June 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    78259-plasmid-map-sequence-id-149444

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Showing: 641 - 680 of 3556 results