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Plasmid#154356PurposeBacterial expression of MTalphaDepositorInsertMTalpha
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHES553 [prGal1-RAS2(KAN) pnh604]
Plasmid#103846PurposeYeast Expression PlasmidDepositorInsertpGal1-Ras2(KAN)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-LINKER
Plasmid#52685PurposeSame as 41023, with additional linkerDepositorInserttetO7-Pcyc1+LINKER
UsePromoter replacementTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTF260 (pFA6a-PSc-6xGly-KanMX)
Plasmid#44086DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags(XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZC12 (pFA6a-PSc-6xGly-V5-KanMX)
Plasmid#44091DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, ADH1 transcription terminator, KanM…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pXTK-BB3 [Backbone_repABC-pMlb-KanR_PartTypeBB]
Plasmid#229389PurposeXanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_repABC-pMlb-KanR_PartTypeBB and an sfGFP dropoutDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXTK-BB2 [Backbone_RK2-KanR-TetR_PartTypeBB]
Plasmid#229388PurposeXanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_RK2-KanR-TetR_PartTypeBB and an sfGFP dropoutDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FRB-3HA-KanMX6
Plasmid#228020PurposeFor TurboID-FRB integration; yeast BioID with KanMX6 markerDepositorInsertTurboID-FRB
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-GFPenhancer
Plasmid#202468PurposeExpresses the nanobody GFP enhancer after phosphate depletionDepositorInsertnanobody GFP enhancer
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-GFPminimizer
Plasmid#202469PurposeExpresses the nanobody GFP minimizer after phosphate depletionDepositorInsertnanobody GFP minimizer
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-H6antivenin
Plasmid#202470PurposeExpresses the nanobody H6 antivenin after phosphate depletionDepositorInsertnanobody H6 antivenin
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-1B5
Plasmid#202472PurposeExpresses the nanobody 1B5 after phosphate depletionDepositorInsertnanobody 1B5
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-2E7
Plasmid#202473PurposeExpresses the nanobody 2E7 after phosphate depletionDepositorInsertnanobody 2E7
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-3E3
Plasmid#202474PurposeExpresses the nanobody 3E3 after phosphate depletionDepositorInsertnanobody 3E3
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-C9BoNT-A
Plasmid#202475PurposeExpresses the nanobody C9 BoNT-A after phosphate depletionDepositorInsertnanobody C9 BoNT/A
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-E7BoNT-A
Plasmid#202476PurposeExpresses the nanobody E7 BoNT-A after phosphate depletionDepositorInsertnanobody E7 BoNT/A
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-JM3
Plasmid#202477PurposeExpresses the nanobody JM3 after phosphate depletionDepositorInsertnanobody JM3
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-m36.4
Plasmid#202479PurposeExpresses the nanobody m36.4 after phosphate depletionDepositorInsertnanobody m36.4
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-Re5D06
Plasmid#202480PurposeExpresses the nanobody Re5D06 after phosphate depletionDepositorInsertnanobody Re5D06
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-Re9F06
Plasmid#202481PurposeExpresses the nanobody Re9F06 after phosphate depletionDepositorInsertnanobody Re9F06
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FKBP-3HA-KanMX6
Plasmid#228024PurposeTo integrate TID-FKBP fusion at yeast promoter; yeast BioID with KanMX6 markerDepositorInsertTurboID-FKBP
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-tRNAGln-BbsI-sU6-Kan
Plasmid#213016PurposeVector for Cloning of multiple gRNAs driven by distinct promoters (tRNA-Gln and synthetic U6)DepositorInserttRNAGln promoter, sU6 promoter, gRNA scaffolds
UseTagsExpressionMutationPromotertRNAGln promoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
human KANK2 Ankyrin Repeats pET151
Plasmid#164054PurposeExpresses human KANK2 Ank domain in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 2 (KANK2 Human)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mouse KANK3 Ankyrin Repeats pET151
Plasmid#164056PurposeExpresses mouse KANK3 Ank domain in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 3 (Kank3 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mouse KANK4 Ankyrin Repeats pET151
Plasmid#164057PurposeExpresses mouse KANK4 Ank domain in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 4 (Kank4 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-eDHFR-Kan
Plasmid#188928PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-eDHFR-Kan
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA KanR neo_zhang2.0
Plasmid#167917PurposeLentiviral vector for expressing U6 MS2-sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pMAL-C2X dAmp/Kana+-AFP
Plasmid#154359PurposeBacterial expression of AFPDepositorInsertAFP
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAL-C2X dAmp/Kana+-2MT
Plasmid#154353PurposeBacterial expression of 2MTDepositorInsert2MT
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAL-C2X dAmp/Kana+-2AFP
Plasmid#154358PurposeBacterial expression of 2AFPDepositorInsert2AFP
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
MoClo adapted pUC57-Kan Level 2
Plasmid#109218PurposeNon binary level 2 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
YCe2764 HC_Kan_RFP-Connector entry vector
Plasmid#100636PurposeEMMA toolkit connector-entry vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTF259 (pFA6a-TEV-6xGly-KanMX)
Plasmid#44092DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags(XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX601-GFP
Plasmid#84040PurposeStaphylococcus aureus (SaCas9) conjugated with GFPDepositorInsertSaCas9
UseAAV and CRISPRTagsNLS and T2A-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TRE-MA4-GFP
Plasmid#114380PurposeDoxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.DepositorInsertMLL-AF4 fusion gene (AFF1 Human)
UseTagsGFPExpressionMammalianMutationPromoterTREAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX601-mCherry
Plasmid#84039PurposeStaphylococcus aureus (SaCas9) conjugated with mCherryDepositorInsertSaCas9
UseAAV and CRISPRTagsNLS and T2A-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-PuroDTK.Neo
Plasmid#72307PurposePiggyBac Cassette contains puromycin and TK genesDepositorInsertPuromycin-DTK-neo
UseTagsExpressionBacterial and MammalianMutationPromoterCAG promoterAvailable sinceFeb. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Tet-on Advanced -BGH-PA
Plasmid#114379PurposeExpress reverse tetracycline inducible rtTA transcriptional activatorDepositorInsertrtTA and BGH-pA
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Neo-PuroDTK
Plasmid#84036PurposePiggyBac cassette contains Neomycin, puromycin and TK genesDepositorTypeEmpty backboneUseTagsExpressionBacterial and MammalianMutationPromoterCAG promoterAvailable sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBR BsmAIM Kan + p15A BsaIR Amp
Plasmid#206208PurposeContains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIRDepositorInsertBsamAIM and BsaIR
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-TarHom-Tol2-kanaR-cryaa-CFP
Plasmid#74151PurposeBAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker CeruleanDepositorInsertTol2-kanaR-cryaa-CFP
UseTagsExpressionMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET264-pUC 24xMS2V6 Loxp KANr Loxp
Plasmid#104393PurposeYeast endogenous mRNA taggingDepositorInsert24xMS2V6
UseTagsExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…PromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET251-pUC 12xMS2V6 Loxp KANr Loxp
Plasmid#104392PurposeYeast endogenous mRNA taggingDepositorInsert12xMS2V6
UseTagsExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…PromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-p2A-CreERT2-FRT-kan-FRT
Plasmid#108454PurposeThis plasmid can be used to recombine the mCherry-p2a-CreERT2-pA cassette (or only CreERT2-pA) into a BAC. Also to produce mCherry-p2A-CreERT2 mRNA using SP6.DepositorInsertmCherry-p2a-CreERT2
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBBR1-kan-Ptet-plu-gamma-ET_rhi145
Plasmid#180427PurposeGenome engineering of AgrobacteriumDepositorInsertplu-gamma-ET_rhi145
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTF277 (pFA6a-TEV-6xGly-2xStrep-KanMX)
Plasmid#44094DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags2xStrep tag, 6xGly linker, ADH1 transcription ter…ExpressionMutationPromoterAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBS-TarHom-Tol2-kanaR-cryaa-dsRed
Plasmid#74152PurposeBAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker dsRedDepositorInsertTol2-kanaR-cryaa-dsRed
UseTagsExpressionMutationPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBBR1-kan-Ptet-plu-gamma-ETh_rhi483
Plasmid#180429PurposeGenome engineering of AgrobacteriumDepositorInsertplu-gamma-ETh_rhi483
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only