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Plasmid#226167PurposeUsed to transiently express the pErk reporter in LABEL-seq MAPK activity assays.DepositorInsert3xFlag-tdMCP-Erk2 (MAPK1 Human)
UseTags3xFlag-tdMCPExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInducer-myc-ERK2-R67S/S153D
Plasmid#213597PurposeDoxycycline inducible expression of myc-tagged ERK2-R67S/S153D cDNA in mammilian cellsDepositorInsertMAPK1 (MAPK1 Human)
UseLentiviralTagsMYCExpressionMammalianMutationR67S, S153DPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPuro3.1(+)_Strep-Lrrk2(G2019S)-Myc
Plasmid#161585Purposeexpression of Myc-tagged mouse Lrrk2 containing Parkinson's associated G2019S mutationDepositorInsertLrrk2 (Lrrk2 Mouse)
UseTagsMyc tag and StrepIIExpressionMammalianMutationG2019SPromoterCMVAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPuro3.1(+)_Strep-Lrrk2(D1994S)-Myc
Plasmid#161584Purposeexpression of Myc-tagged kinase-dead mouse Lrrk2 containing D1994S mutationDepositorInsertLrrk2 (Lrrk2 Mouse)
UseTagsMyc tag and StrepIIExpressionMammalianMutationD1994SPromoterCMVAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-ERK2-L4A-MEK1_fusion
Plasmid#39197DepositorUseTagsMycExpressionMammalianMutationL4A = L33A, L37A, L40A, L42APromoterCMVAvailable sinceJuly 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-rat ERK2-L73P/S151D
Plasmid#40819DepositorInsertMAPK1 (Mapk1 Rat)
UseTagsHis6ExpressionMammalianMutationL73P, S151DPromoterCMVAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBACgus4x/cdc37/LRRK2 1867-2186
Plasmid#40364DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTagsHisTevExpressionMutationWT aa1867-2186PromoterpolHAvailable sinceOct. 2, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPICZ-mouseGIRK2-eGFP-StII-6xHis
Plasmid#124277PurposeExpresses mouse GIRK2 as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tagDepositorInsertGIRK2 (Kcnj6 Mouse)
UseTagseGFP-StreptagII-6xHisExpressionYeastMutationPromoterAOX1Available sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-GRK2RH-Nluc L118A
Plasmid#158606PurposePlasmid expressing GRK2(RH)-Nluc L118A in mammalian cellsDepositorInsertmyristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain L118A - linker (GGTGGS) - Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-DYRK2-FullLength-V5-miniTurbo_IDG-K
Plasmid#198010PurposeGateway destination clone of DYRK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertDYRK2-V5-miniTurbo (DYRK2 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPuro3.1(+)_Strep-Lrrk2(delGTPase)-Myc
Plasmid#161587Purposeexpression of Myc-tagged mouse Lrrk2 without GTPase domainDepositorInsertLrrk2 (Lrrk2 Mouse)
UseTagsMyc tag and StrepIIExpressionMammalianMutationdeletion: AA1023-1879PromoterCMVAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPuro3.1(+)_Strep-Lrrk2(delKinase)-Myc
Plasmid#161586Purposeexpression of Myc-tagged mouse Lrrk2 without kinase domainDepositorInsertLrrk2 (Lrrk2 Mouse)
UseTagsMyc tag and StrepIIExpressionMammalianMutationdeletion: AA1871-2540PromoterCMVAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis
Plasmid#124278PurposeExpresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tagDepositorInsertGIRK2 (Kcnj6 Mouse)
UseTagseGFP-StreptagII-6xHisExpressionYeastMutationR201APromoterAOX1Available sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSUMO His6 SUMO SnRK2.2 (20-362)
Plasmid#177845PurposeBacterial Expression of SnRK2.2DepositorInsertSNF1-related protein kinase 2 (SNRK2.2 Mustard Weed)
UseTags6xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag VRK2
Plasmid#20663DepositorInsertVRK2 (VRK2 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBACgus_Sumostar/LRRK2 1867-2161_C2024S/C2025S
Plasmid#40394DepositorInsertsUseBaculovirusTags6xHis and HisTEVExpressionMutationaa1867-2161,C2024S/C2025SPromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pWZL Neo Myr Flag DYRK2
Plasmid#20479DepositorInsertDYRK2 (DYRK2 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBACgus4x/cdc37/LRRK2 1867-2141
Plasmid#40368DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTagsHisTevExpressionMutationWT, aa1867-2141PromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBACgus/LRRK2 1867-2161 C-His
Plasmid#40377DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTags6xHisExpressionMutationWT aa1867-2161PromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBACgus4x/cdc37/LRRK2 1867-2186_G2019S
Plasmid#40365DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTagsHisTevExpressionMutationaa1867-2186, G2019SPromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits