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Showing: 321 - 340 of 16702 results

  3. pFastBac-GFP-dynein-2(D1091–Q4307)

    Plasmid
    #64064
    Purpose
    Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.
    Depositor
    Andrew Carter
    Article
    Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi
    Insert
    Cytoplasmic dynein-2 (DYNC2H1 Human)
    Use
    Tags
    GFP, His tag, and ZZ tag
    Expression
    Insect
    Mutation
    Deleted amino acids 1-1090, extra C-terminal vali…
    Promoter
    Polyhedrin
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    64064-plasmid-map-sequence-id-110915

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  4. AAV-TRE-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

    Plasmid
    #177344
    Purpose
    AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from doxycycine-inducible promoter
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    dead hSaCas9
    Use
    AAV and CRISPR
    Tags
    3x GCN4 peptide, 3xHA, and NLS
    Expression
    Mammalian
    Mutation
    D10A, N580A
    Promoter
    Tetracycline-dependent promoters
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177344-plasmid-map-sequence-id-353629

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  6. SV.signed

    Plasmid
    #133933
    Purpose
    To validate the use of digital signatures in DNA.
    Depositor
    Jean Peccoud
    Insert
    lacZ and digital signature cassette
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133933-plasmid-map-sequence-id-268619

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  7. pSaGuide

    Plasmid
    #64710
    Purpose
    Cloning and expression of Staphylococcus aureus guide RNA
    Depositor
    Kiran Musunuru
    Insert
    Staphylococcus aureus guide RNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    64710-plasmid-map-sequence-id-108923

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  8. pJK554

    Plasmid
    #72453
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72453-plasmid-map-sequence-id-133893

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  10. pet30b-7M SrtA

    Plasmid
    #51141
    Purpose
    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity
    Depositor
    Hidde Ploegh
    Insert
    S.aureus SrtA 7M
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    P94R, E105K, E108Q, D160N, D165A, K190E, K196T
    Promoter
    T7
    Available since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    51141-plasmid-map-sequence-id-211598

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  11. pAIP4_5IHW

    Plasmid
    #234148
    Purpose
    Heterologous protein expression of serine-aspartate repeat-containing protein E in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5IHW
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234148-plasmid-map-sequence-id-464114

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  12. pAIP4_2OKT

    Plasmid
    #234149
    Purpose
    Heterologous protein expression of o-succinylbenzoate synthase (OSB synthase) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2OKT
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234149-plasmid-map-sequence-id-464115

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  13. pJK550

    Plasmid
    #72450
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    inserted Val2
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72450-plasmid-map-sequence-id-133877

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  14. pJK322

    Plasmid
    #73494
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide mutase
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    n/a
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    73494-plasmid-map-sequence-id-135746

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  15. pJK684

    Plasmid
    #71638
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Val mutant (TdPurE2-S38V)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-38 to valine
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71638-plasmid-map-sequence-id-131658

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  16. pJK685

    Plasmid
    #71639
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asn mutant (TdPurE2-S38N)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-38 to asparagine
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71639-plasmid-map-sequence-id-131659

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  17. pJK687

    Plasmid
    #71640
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Met mutant (TdPurE2-K41M)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes lysine-41 to methionine
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71640-plasmid-map-sequence-id-131661

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  18. pJK688

    Plasmid
    #71641
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Arg mutant (TdPurE2-K41R)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes lysine-41 to arginine
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71641-plasmid-map-sequence-id-131662

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  19. pJK695

    Plasmid
    #71644
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His17>Trp mutant (TdPurE2-H17W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-17 to tryptophan
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71644-plasmid-map-sequence-id-131665

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  20. pJK696

    Plasmid
    #71645
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met15>Trp mutant (TdPurE2-M15W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes methionine-15 to tryptophan
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71645-plasmid-map-sequence-id-131666

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Showing: 321 - 340 of 16702 results