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mTAGBFP2 NES visual barcode

Plasmid

#158667

Purpose

tagging clonal populations with NES mTAGBFP2 fluorescent protein. Adding NES to mTAGBFP2 WC

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mTAGBFP2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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mTurquoise2 Peroxisome visual barcode

Plasmid

#158671

Purpose

tagging clonal populations with Peroxisomal mTurquoise2 fluorescent protein.

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mTurquoise2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-ArchT-GFP

Plasmid

#37807

Purpose

Viral expression of ArchT-GFP for optogenetic inhibition

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

AAV; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CamKII

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_KRAS(G12V)-P2A-Hygro_Barcode

Plasmid

#170237

Purpose

Barcoded lentiviral vector to express KRAS (G12V) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

KRAS (KRAS Human)

Use

Lentiviral

Tags

Expression

Mutation

G12V

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pJFRC-20X-UAS-ADARcd

Plasmid

#81173

Purpose

identification of cell-specific targets of RNA binding proteins

Depositor

Michael Rosbash

Article

McMahon et al Cell. 2016 Mar 30. pii: S0092-8674(16)30

Insert

UAS-ADARcd

Use

Tags

Expression

Insect

Mutation

Promoter

UAS

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FRB-VMAc-CSarcin

Plasmid

#70223

Purpose

Expresses VMA intein with C-terminal portion of alpha-sarcin

Depositor

Perry Howard

Article

Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

Insert

alpha Sarcin (C-terminal portion)

Use

Tags

FKBP-rapamycin binding (FRB) domain of the mammal…

Expression

Mammalian

Mutation

Changed Asp 28 to Cys, deleted amino acids 1-27

Promoter

CMV

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
FCK-ss-Prl-Arch-GFP

Plasmid

#22224

Purpose

Lentiviral expression of Arch-GFP, with N-terminal MHC Class I antigen signal sequence (ss) and Prolactin signal sequence (Prl), for optogenetic inhibition.

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

ss-Prl-Arch-GFP

Use

Lentiviral

Tags

GFP, Prl, and ss

Expression

Mammalian

Mutation

Promoter

CaMKII

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_PTEN(C124S)-P2A-Hygro_Barcode

Plasmid

#170248

Purpose

Barcoded lentiviral vector to express PTEN (C124S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

PTEN (PTEN Human)

Use

Lentiviral

Tags

Expression

Mutation

C124S

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_CDK4(R24C)-P2A-Hygro_Barcode

Plasmid

#170222

Purpose

Barcoded lentiviral vector to express CDK4 (R24C) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK4 (CDK4 Human)

Use

Lentiviral

Tags

Expression

Mutation

R24C

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pTRE Tight ArchT-GFP

Plasmid

#79627

Purpose

Tetracycline-dependent expression of ArchT-EGFP in mammalian cells

Depositor

Yasunori Hayashi

Article

Sakaguchi et al PLoS One. 2015 Jun 15;10(6):e0130163. do

Insert

ArchT

Use

Tags

EGFP

Expression

Mammalian

Mutation

ArchT is a more sensitive version of Arch (archae…

Promoter

Tet responsive Ptight promoter

Available since

July 1, 2016

Availability

Academic Institutions and Nonprofits only
pLJM60-S6K1(T389S) barcoded

Plasmid

#48800

Purpose

expresses mutant S6K1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

S6K1 T389S (Rps6kb1 Rat)

Use

Lentiviral

Tags

FLAG and barcode: GGTACC

Expression

Mammalian

Mutation

T389S

Promoter

CMV

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only
LV_EF1a_Caspase8(C360A)-P2A-Hygro_Barcode

Plasmid

#170217

Purpose

Barcoded lentiviral vector to express Caspase8 (C360A) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CASP8 (CASP8 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

C360A

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_ERalpha(Y537S)-P2A-Hygro_Barcode

Plasmid

#170225

Purpose

Barcoded lentiviral vector to express ERalpha (Y537S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

ER (ESR1 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Y537S

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-EF1α1.1-SomArchon-GFP

Plasmid

#153531

Purpose

AAV-mediated expression of SomArchon-GFP under the EF1α1.1 promoter.

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon-GFP

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

Promoter

EF1α1.1

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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PX386 Lactobacillus farciminis Cas9

Plasmid

#68323

Purpose

Lactobacillus farciminis Cas9

Depositor

Feng Zhang

Insert

LfCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pDest-pol2-Smarcd1-Myc

Plasmid

#227716

Purpose

Expresses mouse Smarcd1 protein with a Myc tag from a Pol2 promoter. Has a Blasticidin resistance gene for stable cell line generation and Ampicilin for plasmid propagation.

Depositor

Kent Hunter

Article

Ross et al bioRxiv 2024.01.24.577061

Insert

Smarcd1 (Smarcd1 Mouse)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

pol2

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(SMARCA2(47))-PGKpuro2ABFP-W

Plasmid

#200510

Purpose

Lentiviral vector expressing gRNA targeting human SMARCA2

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

SMARCA2(47) (SMARCA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(SMARCA4(15))-PGKpuro2ABFP-W

Plasmid

#200462

Purpose

Lentiviral vector expressing gRNA targeting human SMARCA4

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

SMARCA4(15) (SMARCA4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(SMARCA4(43))-PGKpuro2ABFP-W

Plasmid

#200464

Purpose

Lentiviral vector expressing gRNA targeting human SMARCA4

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

SMARCA4(43) (SMARCA4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(SMARCA2(46))-PGKpuro2ABFP-W

Plasmid

#200465

Purpose

Lentiviral vector expressing gRNA targeting human SMARCA2

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

SMARCA2(46) (SMARCA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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