We narrowed to 5,545 results for: donor

1. AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA

   Plasmid

   #97311

   Purpose

   MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb MMEJ donor
   

   Use

   AAV and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. gfap-EGFP donor

   Plasmid

   #65564

   Purpose

   Intron targeting-mediated EGFP knockin at the zebrafish gfap locus

   Depositor

   Jiu-lin Du

   Article

   Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

   Insert

   gfap-EGFP (gfap Zebrafish)
   

   Use

   Zebrafish injection

   Promoter

   none

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Halo-Erk2 donor

   Plasmid

   #113104

   Purpose

   Donor plasmid for knock-in HaloTag fusing to the N-terminal of mouse Erk2 coding sequence

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   N terminal Erk2 donor region including homology arms and HaloTag
   

   Use

   Synthetic Biology

   Available Since

   Aug. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSL0747 (pDonor_L-MmeI)

   Plasmid

   #130647

   Purpose

   Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchCAST donor DNA (L*)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Mutation

   TGTTGATG --> TGTTGGAG

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

   Plasmid

   #97312

   Purpose

   HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. Lenti backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb HMEJ donor
   

   Use

   Lentiviral and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL0746 (pDonor_R-MmeI)

   Plasmid

   #130646

   Purpose

   Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated right end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchCAST donor DNA (R*)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Mutation

   TGTTGATA --> TGTTGGAA

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330 _HITI donor B6

   Plasmid

   #172847

   Purpose

   DRS-2 sgRNA expression under a U6 promotor and HITI donor B6 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-2 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   DRS-2 sgRNA and HITI donor (phase 1) encoding mEGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. V7 pDNR Dual - Donor

   Plasmid

   #11689

   Depositor

   Tony Pawson

   Article

   Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

   Type

   Empty backbone

   Use

   Cre/Lox; Donor vector

   Available Since

   May 24, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. Id2-Halo donor

   Plasmid

   #113116

   Purpose

   Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Id2 coding sequence

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   Id2 donor region including homology arms and HaloTag
   

   Use

   Synthetic Biology

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV_Sox2 HMEJ donor

    Plasmid

    #97322

    Purpose

    HMEJ donor for fusing a p2A-mCherry reporter to mouse Sox2. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Sox2 HMEJ donor
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA

    Plasmid

    #97310

    Purpose

    NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb NHEJ donor
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSL0721 (pDonor_L1-105)

    Plasmid

    #130645

    Purpose

    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened left end. Total transposon size = 933 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCAST donor DNA (short L)
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV_Donor/Reporter-COL4A5

    Plasmid

    #130280

    Purpose

    COL4A5 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A5 donor template

    Depositor

    Silvestro Conticello

    Article

    Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

    Insert

    mCherry-eGFP
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTLR-donor-UAS

    Plasmid

    #119001

    Purpose

    Repair template for the TLR reporter with 10 UAS binding sites for yeast Gal4

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    mVenus
    

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTLR-donor-tetO

    Plasmid

    #119000

    Purpose

    Repair template for the TLR reporter with 7 tet operator elements

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    mVenus
    

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV_Dbh HMEJ donor

    Plasmid

    #97320

    Purpose

    HMEJ donor for fusing a p2A-mCherry-WPRE reporter to mouse Dbh. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Dbh HMEJ donor
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. mGDF8 donor mCherry-PA

    Plasmid

    #72596

    Purpose

    Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin cassette.

    Depositor

    Renzhi Han

    Article

    Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

    Insert

    mCherry-puromycin cassetter
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

18. WT FLAG-HaloTag-RTEL1_DonorVector

    Plasmid

    #247170

    Purpose

    HR Donor for WT C-terminal FLAG-HaloTag-RTEL1 fusion in human cells. Includes Puro selection marker.

    Depositor

    Thomas Cech

    Article

    Wu et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    WT FLAG-HaloTag-RTEL1 (RTEL1 Human)
    

    Promoter

    Endogenous RTEL1 promoter

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLP857_α-syn:oScarlet KI donor

    Plasmid

    #239403

    Purpose

    AAV vector for SpCas9-mediated CRISPR KI of oScarlet at the C-terminus of mouse α-syn

    Depositor

    Subhojit Roy

    Article

    Parra-Rivas et al STAR Protoc. 2025 Jul 21;6(3):103945. do

    Insert

    oScarlet
    

    Use

    AAV and CRISPR

    Tags

    oScarlet

    Expression

    Mammalian

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. Bxb1-GA-CAG Donor

    Plasmid

    #241374

    Purpose

    Bxb1-GA donor plasmid with CAG promoter, WPRE and polyA signal for constitutive transgene expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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