We narrowed to 21,799 results for: Sis

1. Myc-CD4-mCherry-HA-FRB

   Plasmid

   #100740

   Purpose

   To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   CD4
   

   Use

   Tags

   FRB, HA, Myc, and mCherry

   Expression

   Mammalian

   Mutation

   Engineered to remove large part of extracellular …

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTHsp18.2 (GB0035)

   Plasmid

   #68186

   Purpose

   Provides the Arabidopsis thaliana heat shock protein 18.2 terminator as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtHsp18.2 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWZLblasti-CTR1M154A

   Plasmid

   #53158

   Purpose

   Expresses human CTR1 M154A from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   CTR1 (SLC31A1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   M154A

   Promoter

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pDGE497

   Plasmid

   #153249

   Purpose

   M6E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA-T-intron(Neo)-G(FLLFLNL)

   Plasmid

   #40021

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   T (ATG)
   GFP
   beta-globin intron
   Neo
   

   Use

   Tags

   Expression

   Mutation

   deleted nucleotides 1-274 and insertion of FRT, …

   Promoter

   CAG (chicken beta globin promoter and CMV enhance…

   Available since

   Oct. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. SEPLuc-IRES-Antares

   Plasmid

   #141085

   Purpose

   precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   SEPLuc-EMCV IRES-Antares
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPAtUbq10 (GB0223)

   Plasmid

   #68174

   Purpose

   Provides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtUbq10 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPAct2noUTR (GB0192)

   Plasmid

   #68177

   Purpose

   Provides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtAct2 promoter (no UTR)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Antares-SEPLuc

   Plasmid

   #141086

   Purpose

   precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   Antares-T2A-puromycin-EMCV IRES-SEPLuc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 66 to 91 on the Luciferas…

   Promoter

   CMV

   Available since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTAtUbq3 (GB0219)

    Plasmid

    #68192

    Purpose

    Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtUbq3 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHyg-AID(31-114)-9myc

    Plasmid

    #99517

    Purpose

    Truncated C-terminal AID-9myc degron cassette with HygR marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Article

    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

    Insert

    IAA17(31-116) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    9xMyc

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR-IAA17_no stop

    Plasmid

    #83855

    Purpose

    Gateway donor vector for IAA17 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    IAA17 (AXR3 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. JW616

    Plasmid

    #64805

    Purpose

    CUC2 expression in yeast

    Depositor

    Detlef Weigel

    Article

    Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

    Insert

    CUC2 (CUC2 Mustard Weed)
    

    Use

    Tags

    GAL4 Activation Domain, HA, and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDB179-LCC-ICCG-I6M

    Plasmid

    #218690

    Purpose

    Encodes codon optimized LCC-ICCG I6M enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    LCC-ICCG
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDest-pol2-Smarcd1-Myc

    Plasmid

    #227716

    Purpose

    Expresses mouse Smarcd1 protein with a Myc tag from a Pol2 promoter. Has a Blasticidin resistance gene for stable cell line generation and Ampicilin for plasmid propagation.

    Depositor

    Kent Hunter

    Article

    Ross et al bioRxiv 2024.01.24.577061

    Insert

    Smarcd1 (Smarcd1 Mouse)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    pol2

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDB179-PES-H1-L92F-Q94Y

    Plasmid

    #218705

    Purpose

    Encodes codon optimized PES-H1 L92F-Q94Y enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    PES-H1
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    L92F-Q94Y

    Promoter

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMBA332

    Plasmid

    #214744

    Purpose

    Low copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23119-RiboJ-RBS(21992)-gfpmut3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDB179-LCC-ICCG-DAQI

    Plasmid

    #218689

    Purpose

    Encodes codon optimized LCC-ICCG DAQI enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    LCC-ICCG-DAQI
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDB179-TfCut2-L32E-S113E-T237Q

    Plasmid

    #218696

    Purpose

    Encodes codon optimized TfCut2 L32E-S113E-T237Q enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    TfCut2
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    L32E-S113E-T237Q

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDB179-LCC-ICCG-RIP

    Plasmid

    #218688

    Purpose

    Encodes codon optimized LCC-ICCG RIP enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    LCC-ICCG-RIP
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge