We narrowed to 15,769 results for: LEA

1. pCFP

   Plasmid

   #175593

   Purpose

   Lac inducible, AMP Resistant, cerulean gene on a low copy backbone. Used as a negative control to the pMesh1_CFP plasmid.

   Depositor

   Greg Bokinsky

   Article

   Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

   Insert

   CFP (cerulean)
   

   Mutation

   An improved monomeric CFP. See doi: 10.1038/nbt945

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSC130

   Plasmid

   #216364

   Purpose

   xhNup98-Nb3i Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xhNup98-Nb3i
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-NEDD8

   Expression

   Bacterial

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNB648

   Plasmid

   #60140

   Purpose

   NanoLuc luciferase with a nuclear localization signal (NLS) driven by MET17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   NLuc
   

   Tags

   Nuclear localization signal (NLS)

   Expression

   Yeast

   Promoter

   MET17pr

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pICSL50005

   Plasmid

   #117536

   Purpose

   Level0 Golden Gate part: c-terminal tag, YFP

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   c-term tag YFP
   

   Use

   Synthetic Biology

   Mutation

   G65S, L68V, A72S, Y203T, L231H

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-deltaCKAR

   Plasmid

   #31526

   Depositor

   Alexandra Newton

   Article

   Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

   Insert

   Delta-selective C kinase activity reporter
   

   Tags

   CFP and YFP

   Expression

   Mammalian

   Available Since

   Oct. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pBT140

   Plasmid

   #27493

   Depositor

   Liqun Luo

   Article

   Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

   Insert

   Nuclear localization signal-Cre
   

   Tags

   nuclear localization signal

   Expression

   Mammalian

   Available Since

   Feb. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA.PKD.deltaPH

   Plasmid

   #10811

   Depositor

   Alex Toker

   Article

   Storz et al J Biol Chem. 2003 May 16. 278(20):17969-

   Insert

   PKD (PRKD1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PH domain deleted (amino acids 417-545); F94I, V4…

   Promoter

   CMV

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

8. pB6N_RB38-Mt-tk_v8_AGKS

   Plasmid

   #191514

   Purpose

   Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (N-terminal DddAN, Right)

   Depositor

   David Liu

   Article

   Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

   Insert

   v8 ZF-DdCBE RB38-Mt-tk AGKS
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB7N_LT51-Mt-tk_v8_AGKS

   Plasmid

   #191515

   Purpose

   Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (N-terminal DddAC, Left)

   Depositor

   David Liu

   Article

   Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

   Insert

   v8 ZF-DdCBE LT51-Mt-tk AGKS
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMINTC3GH (Hyg)

    Plasmid

    #110077

    Purpose

    Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-rBcan

    Plasmid

    #18958

    Depositor

    Mariano Viapiano

    Article

    Viapiano et al J Neurooncol. 2008 Jul . 88(3):261-72.

    Insert

    Brevican (Bcan Rat)
    

    Expression

    Mammalian

    Available Since

    Dec. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA-Flag-RCC1-polyA

    Plasmid

    #59762

    Purpose

    Express Flag-tagged RCC1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    RCC1
    

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMINTNH3

    Plasmid

    #110074

    Purpose

    Integrative vector for expression in mycobacteria based on the Tet promoter. Contains an N-terminal His10 Tag followed by a 3C cleavage site

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    His10 Tag - 3C cleavage site

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEPOR0SP0020

    Plasmid

    #117531

    Purpose

    Level0 Golden Gate part: CDS, SaCas9

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SaCas9 (with stop codon)
    

    Use

    CRISPR

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAG.3xHalo-GFP-SPC25

    Plasmid

    #176206

    Purpose

    Kinetochore anchor for TMP-Halo dimerization system

    Depositor

    Michael Lampson

    Article

    Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

    Insert

    3xHalo-GFP-SPC25
    

    Expression

    Mammalian

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBBI

    Plasmid

    #65795

    Purpose

    Vector for iCre mRNA synthesis

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al Transgenic Res. 2013 Aug;22(4):873-5. do

    Insert

    iCre
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS423-TyrSgH

    Plasmid

    #163973

    Purpose

    Helper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoter

    Depositor

    Jiazhang Lian

    Article

    Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. PEP102E

    Plasmid

    #97388

    Purpose

    binary construct of nuclear-targeted sfGFP1-10 for plant expression

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

    Insert

    sfGFP1-10OPT
    

    Tags

    Nuclear target

    Expression

    Plant

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMINTC3GH (Kan)

    Plasmid

    #110076

    Purpose

    Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMBP-LbCas12a H759A

    Plasmid

    #113518

    Purpose

    Expresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    LbCas12a
    

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    H759A

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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