We narrowed to 5,560 results for: chia

1. pCDNA5-TEX264-SIM1_mut-cGFP

   Plasmid

   #220212

   Purpose

   Mammalian expression of TEX264 with SIM1 motif mutated, fused at C-terminus to GFP

   Depositor

   Kristijan Ramadan

   Article

   Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   p.111LIDLY>AAAAA; c.331C>G,332T>C,334A&g…

   Promoter

   CMV/TetO

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV5D HA-Δ37, 115-133, 204-217 PPM1H

   Plasmid

   #207344

   Purpose

   Mammalian expression of HA-Δ37, 115-133, 204-217 PPM1H

   Depositor

   Suzanne Pfeffer

   Article

   Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

   Insert

   PPM1H (PPM1H Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Δ37, Δ115-133, Δ204-217

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQE-80L 2xHis-LRRK2 Armadillo E240R

   Plasmid

   #207345

   Purpose

   Bacterial expression of LRRK2 Armadillo

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   LRRK2 Armadillo (LRRK2 Human)
   

   Use

   Tags

   2xHis

   Expression

   Bacterial

   Mutation

   E240R; Armadillo domain only (aa 1-552)

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE-80L 2xHis-LRRK2 Armadillo K439E

   Plasmid

   #207346

   Purpose

   Bacterial expression of LRRK2 Armadillo

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   LRRK2 Armadillo (LRRK2 Human)
   

   Use

   Tags

   2xHis

   Expression

   Bacterial

   Mutation

   K439E; Armadillo domain only (aa 1-552)

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE-80L 2xHis-LRRK2 Armadillo F283A

   Plasmid

   #207348

   Purpose

   Bacterial expression of LRRK2 Armadillo

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   LRRK2 Armadillo (LRRK2 Human)
   

   Use

   Tags

   2xHis

   Expression

   Bacterial

   Mutation

   F283A; Armadillo domain only (aa 1-552)

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV Flag-LRRK2 K17A/K18A/R1441G

   Plasmid

   #186013

   Purpose

   Flag-LRRK2 K17A/K18A/R1441G

   Depositor

   Suzanne Pfeffer

   Article

   Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

   Insert

   Flag-LRRK2 K17A/K18A/R1441G (LRRK2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See depositor's comments below.

   Promoter

   Available since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-guide-puro mBltp1 (KIAA1109) - 1

   Plasmid

   #198489

   Purpose

   lentiviral stable expression of mBltp1 (KIAA1109) gRNA 1

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mBltp1 (4932438A13Rik Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-guide-puro mBltp1 (KIAA1109) - 2

   Plasmid

   #198490

   Purpose

   lentiviral stable expression of mBltp1 (KIAA1109) gRNA 2

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mBltp1 (4932438A13Rik Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT22gcfUbm5En25EE4PCh(#552)

   Plasmid

   #184092

   Purpose

   cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:

   Insert

   5myc-chkEn2(5E)-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   5xMyc

   Expression

   Mutation

   chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …

   Promoter

   Zf-Ubi

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT22Ubm4Eng2bKRPC(#493)

    Plasmid

    #184091

    Purpose

    cyclofen-inducible fish Eng2b (mut) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:

    Insert

    4myc-ZfEng2b(WW>KK)-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    4xMyc

    Expression

    Mutation

    ZfEng2b: W141K, W144K; ERT2: G400V, M543A, L544A,…

    Promoter

    Zf-Ubi

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti_CRISPRi_sgAR10

    Plasmid

    #167003

    Purpose

    Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

    Depositor

    Gian-Paolo Dotto

    Article

    Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

    Insert

    AR Promoter sgRNA 10 (AR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET15-His-∆NGlpG(C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G)

    Plasmid

    #155134

    Purpose

    Expresses N-terminally His-tagged E. coli GlpG C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G variant (residues 87-276) from pET15b

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C104A/D243G/L244G/F245G/M247C/S248G/M249G/A250G, …

    Promoter

    T7

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. rat brain inositol 1,4,5-trisphosphate 3-kinase A (Clone C5, partial sequence from V66)

    Plasmid

    #122450

    Purpose

    Expression of inositol 1,4,5-trisphosphate 3-kinase A

    Depositor

    Christophe Erneux

    Article

    Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.

    Insert

    inositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)
    

    Use

    Tags

    beta-galactosidase

    Expression

    Bacterial

    Mutation

    coding sequence from V66 till stop codon

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. N295A (EcN), E166Q (EcN) MetNI LmH

    Plasmid

    #118269

    Purpose

    N295A and E166Q Mutation of MetN in Methionine ABC Transporter with 6x His Tag. Used to solve crystal structure

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetI (metI Escherichia coli)
    MetN (E166Q ; N295A) (metN Escherichia coli)
    

    Use

    Tags

    6x His Tag

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 166 to Glutamine (Gln…

    Promoter

    T7 Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBEST-p70a-UTR3-Nterm 6xHis mRFP K15TAG-T500

    Plasmid

    #92319

    Purpose

    Expression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR3

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    Optimized NTerm 6xHis tagged monomeric RFP (mRFP)
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    p70a

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBEST-p70a-UTR1-Nterm 6xHis mRFP K15TAG-T500

    Plasmid

    #92318

    Purpose

    Expression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR1

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    Optimized NTerm 6xHis tagged monomeric RFP (mRFP)
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    p70a

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBEST-p70a-UTR1-NTerm 6xHis-zmADH V86TAG-T500

    Plasmid

    #92230

    Purpose

    Expression of zmADH mutant V86TAG for ncAA incoporation using the p70a promoter and UTR1

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    NTerm 6xHis Tagged zmADH
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Residue V86 mutated into TAG (Amber) stop codon f…

    Promoter

    p70a

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAc-GFP-dSUMOdeltaC

    Plasmid

    #85697

    Purpose

    expression of GFP-SUMO1 delta C in Drosophila cells

    Depositor

    Giannino Del Sal, Licio Collavin

    Article

    Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

    Insert

    SUMO (Sumo Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    non-conjugatable SUMO, lacking the C-terminal gly…

    Promoter

    pAc5

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pETM6-1D4-mCherry

    Plasmid

    #73423

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1D4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 1D4 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1D4 (orthogonal T7-lac variant)

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETM6-4A6-mCherry

    Plasmid

    #73424

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4A6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 4A6 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P4A6 (orthogonal T7-lac variant)

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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