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pVD1 tRNA-gRNA position E2 (GB2239)

Plasmid

#160561

Purpose

tRNA and scaffold for the assembly of GBoligomers for position [2-3] of a polycistronic tRNA-gRNA

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

tRNA-gRNA position E2 (Multiplexing Edit)

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pET30a-SARS-CoV2-ORF3b (S23Q)

Plasmid

#165088

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)

Expression

Bacterial

Mutation

Stop codon to glutamine at 23rd amino acid

Available Since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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SGCD-His-bio

Plasmid

#52333

Purpose

Expresses full-length Delta-sarcoglycan ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

SGCD (SGCD Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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F2R-bio-His

Plasmid

#51863

Purpose

Expresses full-length Proteinase-activated receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

F2R (F2R Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pGL4_CRE-CMVmin-BC0491-luc2

Plasmid

#227133

Purpose

Barcoded assay, cAMP/Ca2+ sensor; barcode BC0491

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

6x clustered CRE element linked to CMV minimal promoter driving barcode 0491 and a luciferase reporter gene

Expression

Mammalian

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_ER-RE_MLPmin_BC1065-luc2

Plasmid

#227111

Purpose

Estrogen receptor response element for luciferase and barcode assays; barcode BC1065

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

12x clustered ER response element linked to MLP minimal promoter driving barcode 1065 and a luciferase reporter gene

Use

AAV

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_AR-RE_MLPmin_BC1014-luc2

Plasmid

#227108

Purpose

Androgen receptor response element for luciferase and barcode assays; barcode BC1014

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

8x clustered AR response element linked to MLP minimal promoter driving barcode 1014 and a luciferase reporter gene

Use

AAV

Expression

Mammalian

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_ER-RE_MLPmin_BC1063-luc2

Plasmid

#227109

Purpose

Estrogen receptor response element for luciferase and barcode assays; barcode BC1063

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

12x clustered ER response element linked to MLP minimal promoter driving barcode 1063 and a luciferase reporter gene

Use

AAV

Expression

Mammalian

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_NFAT-RE-CMVmin-BC0538-luc2

Plasmid

#227140

Purpose

Barcoded assay, Ca2+ sensor; barcode BC05038

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

6x clustered NFAT element linked to CMV minimal promoter driving barcode 05038 and a luciferase reporter gene

Expression

Mammalian

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-GFP [N86/34R]

Plasmid

#220419

Purpose

Mammalian Expression Plasmid of anti-GFP (Jellyfish) IgG2a R-mAb. Derived from hybridoma N86/34

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody.

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pTT3-ecdCD86-ST3-His

Plasmid

#210664

Purpose

Expression of the extracellular domain of human CD86 fused to Spytag003 + Histag in HEK293 (or similar)

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

Extracellular domain of human CD86 fused to Spytag003 and Histag (CD86 Human)

Tags

Histag and Spytag003

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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LLP790_αGCN4-EZH2-FL-CO

Plasmid

#211784

Purpose

SunTag counterpart binding domain, aGCN4, fused to polycomb repressive complex subunit EZH2, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

SnoopCatcher-KRAB

Tags

2xOLLAS

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect KRAB e…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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LLP778_Traptavidin-p65HSF1-CO

Plasmid

#211771

Purpose

AviTag counterpart binding domain, Traptavidin, fused to transcriptional activator p65HSF1, with sfGFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

Traptavidin-p65HSF1

Tags

3xV5

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect p65HSF…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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LLP776_SpyCatcher-p65HSF1-CO

Plasmid

#211769

Purpose

SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional activator p65HSF1, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

SpyCatcher-p65HSF1

Tags

3xFLAG

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect p65HSF…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-GFAP [N206A/8R-2b]

Plasmid

#199410

Purpose

Mammalian expression plasmid of Anti-GFAP [N206A/8R-2b] (Human). Derived from hybridoma N206A/8.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-GFAP (Human) recombinant mouse monoclonal antibody (GFAP Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Rem2 [N324/2]

Plasmid

#190312

Purpose

Mammalian Expression Plasmid of anti-Rem2 (Rat). Derived from hybridoma N324/2.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Rem2 (Rattus norvegicus) recombinant Mouse monoclonal antibody (Rem2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-MCS-eDHFR(69K6)-EGFP

Plasmid

#172101

Purpose

Mammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFP

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

22aa-eDHFR(69K6)-EGFP

Expression

Mammalian

Mutation

eDHFR(69K6): Hexalysine (K6) sequence is inserted…

Promoter

CMV

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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Kv2.1 K+ channel scFv [K39/25]

Plasmid

#182067

Purpose

Mammalian Expression of Kv2.1 K+ channel scFv. Derived from hybridoma K39/25.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Kv2.1 K+ channel (Rattus norvegicus) (Kcnb1 Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-GIT1 [N39B/8R]

Plasmid

#177545

Purpose

Mammalian Expression Plasmid of anti-GIT1 (Rat). Derived from hybridoma N39B/8.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GIT1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Git1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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TMED5-bio-His

Plasmid

#53338

Purpose

Expresses full-length Transmembrane emp24 domain-containing protein 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

TMED5 (TMED5 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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