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pGEX Socs7-SH2

Plasmid

#46509

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Socs7 (SOCS4 Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 280-386

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Nsp3-SH2

Plasmid

#46461

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Nsp3 (SH2D3C Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 212-330

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pIS50 KIF2 3'UTR

Plasmid

#14493

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

KIF2 3'UTR (KIF2A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry MALAT1_Exon.2

Plasmid

#78541

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 (for expressing sgRNA) and U6 promoter

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry MALAT1_Exon.4

Plasmid

#78543

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 and U6 (for expressing sgRNA)

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMT3-RhoAC

Plasmid

#100032

Purpose

This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with adenylyl cyclase activity.

Depositor

Daniel Oprian

Article

Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

Insert

RhoAC

Tags

1D4 (TETSQVAPA)

Expression

Mammalian

Mutation

E497K/C566D

Promoter

Adenovirus major late promoter

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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mTFP1-CENPB-N-22

Plasmid

#55470

Purpose

Localization: Nucleus/Centromeres, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

CENPB (CENPB Human)

Tags

mTFP1

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pMT3-RhoGC E254D

Plasmid

#100033

Purpose

This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 390 nm.

Depositor

Daniel Oprian

Article

Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

Insert

RhoGC E254D

Tags

1D4 (TETSQVAPA)

Expression

Mammalian

Mutation

E254D

Promoter

Adenovirus major late promoter

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only
tdEos-VASP-5

Plasmid

#57685

Purpose

Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

VASP

Tags

tdEos

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mCardinal-Cx43-7

Plasmid

#56157

Purpose

Localization: Gap Junctions, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Cx43

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mKO-Tubulin-6

Plasmid

#57850

Purpose

Localization: Microtubules, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Article

Shroff et al Proc Natl Acad Sci U S A. 2007 Dec 18;10

Insert

Tubulin (TUBA1B Human)

Tags

mKO

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
tdEos-PMP-N-10

Plasmid

#57658

Purpose

Localization: Peroxisomes, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Huang et al Nat Methods. 2013 Jul;10(7):653-8. doi:

Insert

PMP (PXMP2 Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mTFP1-H3.3-N-14

Plasmid

#55490

Purpose

Localization: Nucleus/Histones, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

H3.3 (H3f3b Mouse)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
BacPAK Flag-Adrm1

Plasmid

#19419

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

Insert

adhesion regulating molecule 1 (ADRM1 Human)

Tags

Flag and His

Expression

Insect

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pET-51b(+)_His10x-TEV-Rho-tag

Plasmid

#249665

Purpose

Expression of rhodamine-binding protein tag in bacterial cells

Depositor

Kai Johnsson, Richard Wombacher

Article

Kompa et al J Am Chem Soc. 2025 Dec 30. doi: 10.1021

Insert

Rho-tag

Expression

Bacterial

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)_His10x-TEV-SiR-tag

Plasmid

#249666

Purpose

Expression of silicon rhodamine-binding protein tag in bacterial cells

Depositor

Kai Johnsson, Richard Wombacher

Article

Kompa et al J Am Chem Soc. 2025 Dec 30. doi: 10.1021

Insert

SiR-tag

Expression

Bacterial

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pSFV_eGFP-P2A-luc2pre (3'UTR)

Plasmid

#240254

Purpose

Non-attenuated SFV vector with eGFP-LUC transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

EGFP-P2A-firefly luciferase

Expression

Mammalian

Promoter

SFV subgenomic promoter

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pSFV_rTetR-VP16 (3'UTR)_RLEloop

Plasmid

#240257

Purpose

Attenuated PROTEUS vector with rtTA-3G transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

rTetR-VP16

Expression

Mammalian

Mutation

VP16[aa437-447]

Promoter

SFV subgenomic promoter

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pSFV_TetR-VP16 (3'UTR)_RLEloop

Plasmid

#240259

Purpose

Attenuated PROTEUS vector with tTA transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

TetR-VP16

Expression

Mammalian

Mutation

VP16[aa437-447]

Promoter

SFV subgenomic promoter

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_CD86-Rho-tag

Plasmid

#249669

Purpose

Expression of monomeric rhodamine-binding protein tag on the cell surface in mammalian cells

Depositor

Kai Johnsson, Richard Wombacher

Article

Kompa et al J Am Chem Soc. 2025 Dec 30. doi: 10.1021

Insert

CD86-Rho-tag

Expression

Mammalian

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pYO829

Plasmid

#242492

Purpose

Expression of ScAtg38 Δ(182 to 209)-3xFLAG under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Mutation

deletion of aa 182 to 209

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pYO827

Plasmid

#242491

Purpose

Expression of ScAtg38 Δ(123 to 181)-3xFLAG under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Mutation

deletion of aa 123 to 181

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO815

Plasmid

#242490

Purpose

Expression of ScAtg38 Δ(182 to 226)-3xFLAG under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Mutation

deletion of aa 182 to 226

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO814

Plasmid

#242489

Purpose

Expression of ScAtg38 Δ(123 to 226)-3xFLAG under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Mutation

deletion of aa 123 to 226

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO845

Plasmid

#242494

Purpose

Expression of ScAtg38 Δ(210 to 226)-3xFLAG under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Mutation

deletion of aa 210 to 226

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO844

Plasmid

#242493

Purpose

Expression of ScAtg38 Δ(123 to 209)-3xFLAG under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Mutation

deletion of aa 123 to 209

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO843

Plasmid

#242487

Purpose

Expression of ScAtg38 Δ(210 to 226)-2xEGFP under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

2xEGFP

Expression

Yeast

Mutation

deletion of aa 210 to 226

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO835

Plasmid

#242484

Purpose

Expression of ScAtg38 Δ(123 to 181)-2xEGFP under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

2xEGFP

Expression

Yeast

Mutation

deletion of aa 123 to 181

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO842

Plasmid

#242486

Purpose

Expression of ScAtg38 Δ(123 to 209)-2xEGFP under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

2xEGFP

Expression

Yeast

Mutation

deletion of aa 123 to 209

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO817

Plasmid

#242483

Purpose

Expression of ScAtg38 Δ(182 to 226)-2xEGFP under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

2xEGFP

Expression

Yeast

Mutation

deletion of aa 123 to 226

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO836

Plasmid

#242485

Purpose

Expression of ScAtg38 Δ(182 to 209)-2xEGFP under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

2xEGFP

Expression

Yeast

Mutation

deletion of aa 182 to 209

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p-HITI-GFP-Ckm

Plasmid

#226119

Purpose

HITI insert construct with Ckm gRNA and GFP transgene

Depositor

Christopher Nelson

Article

Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

Insert

Ckm (Ckm Mouse)

Use

CRISPR

Tags

eGFP

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
TTR A120S

Plasmid

#238111

Purpose

bacterial expression of human TTR mutant

Depositor

Peter Wright

Article

Sun et al Protein Sci. 2024 Sep;33(9):e5101. doi:

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

A120S

Promoter

T7

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TTR A120L

Plasmid

#238110

Purpose

bacterial expression of human TTR mutant

Depositor

Peter Wright

Article

Sun et al Protein Sci. 2024 Sep;33(9):e5101. doi:

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

A120L

Promoter

T7

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TTR A25T

Plasmid

#238114

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Sun et al J Am Chem Soc. 2024 Jan 10;146(1):532-54

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

A25T

Promoter

T7

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-tev-G(TTR A25T)

Plasmid

#238116

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Sun et al J Am Chem Soc. 2024 Jan 10;146(1):532-54

Insert

TTR (TTR Human)

Tags

His6tev-G

Expression

Bacterial

Mutation

A25T

Promoter

T7

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR_HA-spGFP11

Plasmid

#240224

Purpose

Gateway entry clone for cloning in insert sequences by gibson assembly to create a C-terminal HA-spGFP11 tag. No ATG start codon.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Type

Empty backbone

Use

Gateway shuttle vector

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO979

Plasmid

#235756

Purpose

Protein expression of ScVPS38, untagged + ScVPS30 FRK to DDD at 430-432, untagged

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS38 (VPS38 Budding Yeast)
VPS30 (VPS30 Budding Yeast)

Expression

Yeast

Mutation

FRK430-432DDD

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO359

Plasmid

#235750

Purpose

Protein expression of FL ScVPS34 and FL ScVPS15-ZZ

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS34 (VPS34 Budding Yeast)
VPS15 (VPS15 Budding Yeast)

Tags

3xTEV-ZZ

Expression

Yeast

Mutation

S2A, aa 3-887 and T134A, I851R

Promoter

GAL-TDH3

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO1006

Plasmid

#235724

Purpose

Expression of FL human Beclin 1 in mammalian cells

Depositor

Roger Williams

Article

Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

Insert

Beclin 1 (BECN1 Human)

Expression

Mammalian

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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