We narrowed to 5,107 results for: PEP

1. pAN012

   Plasmid

   #220051

   Purpose

   Reporter plasmid that expresses GFP and firefly luciferase linked with a viral 2A peptide (positive control).

   Depositor

   Blake Wiedenheft

   Article

   Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

   Insert

   GFP-fLuc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR_SFFV*/Puro2a-PAG1-GFP

   Plasmid

   #198223

   Purpose

   Lentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFP

   Depositor

   Eric Davis

   Insert

   PAG1 (PAG1 Human)
   

   Use

   Lentiviral

   Tags

   Followed by EGFP, after a flexible linker and Pre…

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-eGFP-P(11)4

   Plasmid

   #185786

   Purpose

   Encodes enhanced green fluorescent protein linked to P(11)4 self-assembling peptide via a GS-linker sequence

   Depositor

   Lizhong He

   Article

   Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

   Insert

   eGFP-P(11)4
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-ATA-P(11)4

   Plasmid

   #185787

   Purpose

   Encodes enzyme ω-aminotransferase linked to P(11)4 self-assembling peptide via a GS-linker sequence

   Depositor

   Lizhong He

   Article

   Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

   Insert

   ATA-P(11)4
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-GST-P(11)4

   Plasmid

   #185789

   Purpose

   Encodes glutathione S-transferase linked to P(11)4 self-assembling peptide via a GS-linker sequence

   Depositor

   Lizhong He

   Article

   Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

   Insert

   GST-P(11)4
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD-wtBlc

   Plasmid

   #168255

   Purpose

   wild‐type E. coli lipocalin Blc without signal peptide

   Depositor

   Jens Meiler

   Article

   Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:

   Insert

   Lipocalin Blc
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pST50Trc4-CBPNDHFR

   Plasmid

   #63995

   Purpose

   Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   Calmodulin Binding Peptide (CBP); TEV cleavable

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pST50Trc1-CBPHISNDHFR

   Plasmid

   #63945

   Purpose

   Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   6xHis & Calmodulin Binding Peptide (CBP); TEV…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pST50Trc3-CBPNDHFR

   Plasmid

   #63977

   Purpose

   Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   Calmodulin Binding Peptide (CBP); TEV cleavable

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pST50Trc2-CBPHISNDHFR

    Plasmid

    #63963

    Purpose

    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    6xHis & Calmodulin Binding Peptide (CBP); TEV…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET29b-ClbP-S95A

    Plasmid

    #48245

    Purpose

    Untagged, full-length protein with active site mutation Serine95 to Alanine for in vivo peptidase assays

    Depositor

    Emily Balskus

    Article

    Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

    Insert

    ClbP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed Serine 95 to Alanine

    Promoter

    T7

    Available since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pICH37326

    Plasmid

    #50307

    Depositor

    Nicola Patron, Sylvestre Marillonnet

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    signal peptide, NbCRT calreticulin (Nicotiana benthamiana)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV-Mito4x-GCaMP6f

    Plasmid

    #127870

    Purpose

    Fluorescent reporter for mitochondrial matrix Ca2+

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    4 repeats of COX8 signal peptide plus GCaMP6f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-scFv-GCN4-sfGFP-GB1-dWPRE

    Plasmid

    #60907

    Purpose

    The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP for imaging

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    scFv-GCN4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFGET19_Ulp1

    Plasmid

    #64697

    Purpose

    H6-Ulp1 from pHYRS52 into pET-28b(+)

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Insert

    ULP1 (ULP1 Budding Yeast, Saccharomyces cerevisiae)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Ulp1(403-621)

    Promoter

    T7

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. Flag-HA-USP11

    Plasmid

    #22566

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP11 (USP11 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYHY2

    Plasmid

    #216108

    Purpose

    Constitutive expression of secretory BT0294 SP-NanoLuc via BfP1E6-RBS8 promoter-RBS pair in Bacteroides species.

    Depositor

    Shannon Sirk

    Article

    Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

    Insert

    NanoLuc
    

    Use

    Synthetic Biology; E. coli-bacteroides shuttle ve…

    Tags

    3xFLAG tag and BT0294 signal peptide

    Expression

    Bacterial

    Mutation

    Promoter

    BfP1E6 promoter

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE

    Plasmid

    #60906

    Purpose

    The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imaging

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    scFv-GCN4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. psd44-GqQL

    Plasmid

    #46826

    Purpose

    2nd generation lentiviral vector for expression of mutant G protein alpha q in mammalian cells

    Depositor

    Agnese Mariotti

    Insert

    g protein alpha q (GNAQ Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Q209L (The mutation reduces GTPase activity resul…

    Promoter

    PGK promoter

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5-AP-Ub-dGG

    Plasmid

    #232581

    Purpose

    Express Acceptor Peptide (AP) tagged Ubiquitin lacking the two terminal glycines

    Depositor

    Sagar Bhogaraju

    Article

    Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

    Insert

    Ubiquitin
    

    Use

    Tags

    AviTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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