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ATM-Halo HRD

Plasmid

#207082

Purpose

Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous ATM locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

HaloTag followed by a PolyA signal and PuroR cassette flanked by human ATM locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

None

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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2XMyc-LRRK2-Y1699C

Plasmid

#25364

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Tyrosine 1699 mutated to Cysteine

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only
pCDB179-CaPETaseM9

Plasmid

#218707

Purpose

Encodes codon optimized CaPETaseM9 enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

CaPETaseM9

Tags

10xHis

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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His-GFP-Rabphilin

Plasmid

#170865

Purpose

Mammalian expression of His-GFP-Rabphilin.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Rabphilin-3a (RPH3A Bovine)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagD 121L

Plasmid

#19309

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Tags

GST and HA

Expression

Mammalian

Mutation

Q121L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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Rtn2b-mCherry

Plasmid

#186600

Purpose

Encodes human full-length Reticulon 2 isoform B fused to mCherry

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B (RTN2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pBad33_ISAam1_reRNA

Plasmid

#205322

Purpose

Bacterial expression of ISAam1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISAam1 TnpB

Expression

Bacterial

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagC 120L

Plasmid

#19306

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding C (RRAGC Human)

Tags

GST and HA

Expression

Mammalian

Mutation

Q120L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pGFP-E p27 (CC#631)

Plasmid

#15192

Depositor

Connie Cepko

Article

Dyer et al J Neurosci. 2001 Jun 15. 21(12):4259-71.

Insert

p27 (Cdkn1b Mouse)

Use

Retroviral

Tags

Flag and His

Expression

Mammalian

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only

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pQdCas9-sgempty

Plasmid

#159523

Purpose

Expression of dCas9 driven by lux cassette from Vibrio fischeri

Depositor

Radhakrishnan Mahadevan

Article

Liu et al ACS Synth Biol. 2020 Jun 5. doi: 10.1021

Insert

quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dcas9 and sgRNA

Use

CRISPR

Promoter

quorum sensing promoter

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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His-GFP-Rab3a

Plasmid

#170863

Purpose

Mammalian expression of His-GFP-Rab3a.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Rab-3a (Rab3a Mouse)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pKO (Kan)

Plasmid

#110086

Purpose

Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST Rheb1

Plasmid

#19310

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RHEB Ras homolog enriched in brain (RHEB Human)

Tags

GST and HA

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pSV2neo human IFN-gamma

Plasmid

#10954

Depositor

Howard Young

Article

Young et al J Immunol. 1986 Jun 15. 136(12):4700-3.

Insert

interferon-gamma (IFNG Human)

Expression

Mammalian

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pDD283

Plasmid

#66824

Purpose

YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

YPET-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized YPET

Expression

Worm

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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Flag pLJM1 RagD 121L

Plasmid

#19318

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Q121L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-NKX3.1

Plasmid

#42897

Depositor

Edward Gelmann

Article

Steadman et al Nucleic Acids Res. 2000 Jun 15;28(12):23

Insert

NKX3.1 (NKX3-1 Human)

Expression

Mammalian

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO_STf_LamB1

Plasmid

#181999

Purpose

CMV overexpression of SNAP-tag fast fusion to Lamin B1 for nuclear envelope localization in mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

STf-LamB1

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1aL-mCherry-W

Plasmid

#126703

Purpose

Constitutive, ubiquitous mCherry expression.

Depositor

Darrell Kotton

Article

McCauley et al Cell Stem Cell. 2017 Jun 1;20(6):844-857

Insert

mCherry

Use

Lentiviral

Promoter

Ef1aL

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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AAV.hSyn.GLPLight-ctr

Plasmid

#187468

Purpose

Expresses GLPLight-ctr in mammalian cells

Depositor

Tommaso Patriarchi

Article

Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

Insert

GLPLight-ctr

Use

AAV

Tags

Flag tag

Promoter

human Synapsin-1

Available Since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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