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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 4,940 results for: puc

Showing: 3401 - 3420 of 4940 results

  1. Donor_eGP2AP_RC

    Plasmid
    #133784
    Purpose
    Library reporter vector/integration vector. Contains library cloning sites MluI and SpeI upstream of a terminator and downstream of a BxB1 attB site. Library integration occurs through BxB1 site.
    Depositor
    Sriram Kosuri
    Article
    Davis et al Cell Syst. 2020 Jul 22;11(1):75-85.e7. d
    Type
    Empty backbone
    Use
    Tags
    eGFP-P2A-Puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133784-plasmid-map-sequence-id-274492

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  2. pDGE496

    Plasmid
    #153248
    Purpose
    M6 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
    Depositor
    Johannes Stuttmann
    Article
    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153248-plasmid-map-sequence-id-310110

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  3. pDGE495

    Plasmid
    #153247
    Purpose
    M5 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
    Depositor
    Johannes Stuttmann
    Article
    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    153247-plasmid-map-sequence-id-305816

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  5. pZH518

    Plasmid
    #102671
    Purpose
    Tetracycline inducible expression of GFPmut2 using autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102671-plasmid-map-sequence-id-199394

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  6. pFH113

    Plasmid
    #128210
    Purpose
    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
    Depositor
    Vladimir Nekrasov
    Article
    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d
    Insert
    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    128210-plasmid-map-sequence-id-252904

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  9. pAL1

    Plasmid
    #197285
    Purpose
    Shuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195
    Depositor
    Bogumil Karas
    Article
    Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.
    Insert
    Origin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197285-plasmid-map-sequence-id-389699

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  10. pVH613

    Plasmid
    #169602
    Purpose
    Tier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (PCREm4-Nluc-pA::A2-pA::PmPGK1-Fluc-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    cAMP response element-controlled NanoLuc Luciferase expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PCREm4 / PPGK
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169602-plasmid-map-sequence-id-365597

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  11. ATM_N_FS_v2_Donor

    Plasmid
    #214815
    Purpose
    N-terminal tagging of human ATM with 3xFLAG_Twin_Strep
    Depositor
    Yannick Doyon
    Insert
    3xFLAG_2xStrep for tagging ATM at the N-terminus (ATM Human, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG_2xStrep
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    214815-plasmid-map-sequence-id-422878

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  13. pDGE336

    Plasmid
    #153245
    Purpose
    M4 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
    Depositor
    Johannes Stuttmann
    Article
    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    153245-plasmid-map-sequence-id-305800

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  14. pDGE341

    Plasmid
    #153250
    Purpose
    M7 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
    Depositor
    Johannes Stuttmann
    Article
    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    153250-plasmid-map-sequence-id-305845

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  15. pADH139

    Plasmid
    #90987
    Purpose
    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR; use with pADH110 to generate target-specific part 2 of 2 for C.mal LEUpOUT CRISPR system.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    gRNA conserved, C, mal LEU2 2 of 2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90987-plasmid-map-sequence-id-180520

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  20. pFH49

    Plasmid
    #128211
    Purpose
    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
    Depositor
    Vladimir Nekrasov
    Article
    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d
    Insert
    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    128211-plasmid-map-sequence-id-252903

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Showing: 3401 - 3420 of 4940 results