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pLX317-MBNL1

Plasmid

#115443

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

MBNL1 (MBNL1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-NUAK2

Plasmid

#23831

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NUAK2 (NUAK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-FER

Plasmid

#23921

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FER (FER Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MAPKAPK3

Plasmid

#23790

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPKAPK3 (MAPKAPK3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CSNK2B

Plasmid

#23359

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CSNK2B (CSNK2B Human)

Use

Gateway Cloning

Mutation

C138T

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pRSpuro shID3#1

Plasmid

#19163

Depositor

Joan Massague

Article

Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

Insert

small hairpin RNA against inhibitor of DNA binding 3 (ID3 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 16, 2008

Availability

Academic Institutions and Nonprofits only
1113 pSG5L HA PTEN D3

Plasmid

#10752

Depositor

William Sellers

Article

Vazquez et al Mol Cell Biol. 2000 Jul . 20(14):5010-8.

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

D3: S380D, T382D, T383D

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pCdOpt-BMX

Plasmid

#203929

Purpose

This plasmid contains a codon optimized BleMX drug resistance cassette for generating gene deletions in C. glabrata, C. auris, C. albicans or S. cerevisiae

Depositor

Scott Briggs

Article

Gregor et al mSphere. 2023 Nov 6:e0031123. doi: 10.11

Insert

BleMX

Expression

Yeast

Mutation

BleMX was codon optimized for Candida albicans or…

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-MYOCD

Plasmid

#170696

Purpose

doxycycline-inducible overexpression of MYOCD

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

MYOCD (MYOCD Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVTRE3_Clover

Plasmid

#135179

Purpose

AAV expression of TRE(TRE3G)-driven, Clover expression for fluorescent labling

Depositor

Tetsuo Yamamori

Article

Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

Inserts

Clover
TRE3G

Use

AAV

Expression

Mammalian

Mutation

a variant of GFP

Promoter

TRE3G

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup88

Plasmid

#87338

Purpose

To express human Nup88 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP88 (NUP88 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiX2-PURO-shLKB1-Ms

Plasmid

#61231

Purpose

Lentivirus expressing shRNA to mouse LKB1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

shLkb1 (Ms/Hu) (Stk11 Mouse, Human)

Use

Lentiviral

Promoter

H1/TO

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
mCherry KNL1 wt hardened

Plasmid

#45224

Depositor

Iain Cheeseman, Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

mCherry KNL1 resistant to KNL1 siRNA (KNL1 Human)

Tags

TEV S15

Expression

Mammalian

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
pAd GFP HDAC5 S259A/S498A

Plasmid

#32218

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Use

Adenoviral

Tags

GFP

Expression

Mammalian

Mutation

S259A/S498A

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
258/-2.5CAT

Plasmid

#25431

Depositor

Charles P. Emerson Jr.

Article

Goldhamer et al Development. 1995 Mar . 121(3):637-49.

Insert

Myo D Promoter/Enhancer (MYOD1 Human)

Use

Reporter construct

Tags

CAT

Expression

Mammalian

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

Plasmid

#176534

Purpose

Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

CRISPR-associated IscB (Delaware Bay metagenome)

Tags

14xHis-MBP-bdSUMO and TwinStrep

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

Plasmid

#139093

Purpose

Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

dxCas9-NG-VPR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCFP

Plasmid

#28011

Depositor

Lingchong You

Article

Tan et al Nat Chem Biol. 2009 Nov . 5(11):842-8.

Insert

CFP

Expression

Bacterial

Available Since

March 23, 2011

Availability

Academic Institutions and Nonprofits only

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pSH1/M-FGFR3-Fv-Fvls-E

Plasmid

#15287

Depositor

David Spencer

Article

Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

Insert

FGFR3 kinase, FKBP12v36 (Fgfr3 Mouse)

Tags

Myristoylation-targeting domain c-Src (14aa) and …

Expression

Mammalian

Mutation

FGFR3 cytoplasmic domain only FKBP12 F36V

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pMMBGent

Plasmid

#45475

Depositor

Michele Swanson

Article

Hammer et al Mol Microbiol. 1999 Aug;33(4):721-31.

Insert

Gentamycin

Expression

Bacterial

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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