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Showing: 6841 - 6880 of 9862 results

  2. pBS-p85alpha

    Plasmid
    #1407
    Depositor
    Lewis Cantley
    Insert
    PI3K regulatory subunit p85alpha (Pik3r1 Mouse)
    Expression
    Mammalian
    Available Since
    June 16, 2005
    Availability
    Academic Institutions and Nonprofits only

  3. pGEX-4T3-p85beta

    Plasmid
    #1406
    Depositor
    Lewis Cantley
    Insert
    PI3K regulatory subunit p85beta (Pik3r2 Mouse)
    Tags
    GST
    Expression
    Bacterial
    Available Since
    Nov. 14, 2005
    Availability
    Academic Institutions and Nonprofits only

  4. pIREShyg-p50alpha-Flag

    Plasmid
    #1405
    Depositor
    Lewis Cantley
    Insert
    PI3K regulatory subunit p50alpha (Pik3r1 Mouse)
    Tags
    Flag
    Expression
    Mammalian
    Available Since
    June 16, 2005
    Availability
    Academic Institutions and Nonprofits only

  5. STK190_pSTK0B-RiboJ-RBS0

    Plasmid
    #235385
    Purpose
    Very strong RBS in B. subtilis. RiboJ is a self cutting 5' region of the mRNA to avoid interactions with the RBS.
    Depositor
    Tom Ellis
    Insert
    RiboJ-RBS0
    Expression
    Bacterial
    Mutation
    No
    Available Since
    March 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    235385-plasmid-map-sequence-id-502643

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  6. pCMV6-p85alpha-Flag

    Plasmid
    #1399
    Depositor
    Lewis Cantley
    Insert
    PI3K regulatory subunit p85alpha (Pik3r1 Mouse)
    Tags
    Flag
    Expression
    Mammalian
    Available Since
    July 18, 2006
    Availability
    Academic Institutions and Nonprofits only
    1399-plasmid-map-sequence-id-340532

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  7. pCMV6-p55alpha-Flag

    Plasmid
    #1402
    Depositor
    Lewis Cantley
    Insert
    PI3K regulatory subunit p55alpha (Pik3r1 Mouse)
    Tags
    Flag
    Expression
    Mammalian
    Available Since
    July 18, 2006
    Availability
    Academic Institutions and Nonprofits only

  8. STK078_pSTK-0D-L3S3P21b

    Plasmid
    #235413
    Purpose
    Very strong terminator in B. subtilis. Strong synthetic transcriptional terminator in E. coli. Recombinant resistant. Modified to remove BsaI site (Position 49G to 49T).
    Depositor
    Tom Ellis
    Insert
    L3S3P21
    Expression
    Bacterial
    Mutation
    No
    Available Since
    March 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    235413-plasmid-map-sequence-id-502667

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  9. pOpen-ALPI

    Plasmid
    #165555
    Purpose
    CIAP reporter; can be used in spectrophotometric or luminescence assay when appropriate substrate is used
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    CIP (calf intestinal phosphatase)
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165555-plasmid-map-sequence-id-339907

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  10. pOpen-9N7polA (CT)

    Plasmid
    #165502
    Purpose
    9 Degrees North DNA polymerase variant with enhanced ability to incorporate modified substrates
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    9 degrees N-7 DNA Polymerase chain terminating
    Use
    Synthetic Biology
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165502-plasmid-map-sequence-id-338438

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  12. Sc rev7-pRS405/GAL

    Plasmid
    #241259
    Purpose
    Over-express Sc rev7 (Sc Pol zeta subunit) in yeast (integrated)
    Depositor
    Michael O'Donnell
    Insert
    rev7 (REV7 Budding Yeast)
    Expression
    Yeast
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241259-plasmid-map-sequence-id-482061

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  13. OsMATE

    Plasmid
    #117093
    Purpose
    protein expression in yeast
    Depositor
    Geoffrey Chang
    Insert
    AtTPT (ORF-HIS)
    Tags
    His
    Expression
    Yeast
    Promoter
    AOX
    Available Since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    117093-plasmid-map-sequence-id-224955

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  14. pJEX_P1terminase

    Plasmid
    #248951
    Purpose
    phage P1 large terminase subunit under control of a crystal violet inducible promoter
    Depositor
    Jennifer Doudna
    Insert
    P1terminase
    Expression
    Bacterial
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    248951-plasmid-map-sequence-id-504693

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  15. Sc Pol12-pRS406/GAL

    Plasmid
    #241976
    Purpose
    Over-express Sc Pol12 (Sc pol alpha subunit) in yeast (integrated)
    Depositor
    Michael O'Donnell
    Insert
    Pol12 (POL12 Budding Yeast)
    Expression
    Yeast
    Available Since
    Aug. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    241976-plasmid-map-sequence-id-483376

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  16. C85m

    Plasmid
    #120996
    Purpose
    MoClo golden gate assembly CD part for sigmaX (Bacillus subtilis RNA polymerase involved in heat response and biofilm formation). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma X (ECF20_992)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120996-plasmid-map-sequence-id-236125

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  17. pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid
    #215010
    Purpose
    Express a Mitophagy reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    pSu9 (Presequence of Fo-ATPase subunit 9)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Available Since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215010-plasmid-map-sequence-id-429275

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  18. pGR420

    Plasmid
    #213782
    Purpose
    Integrates onto p1 a partial LEU2 sequence to be subsequently used as an integration flank
    Depositor
    Chang Liu
    Insert
    MET15
    Use
    Synthetic Biology
    Expression
    Yeast
    Available Since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213782-plasmid-map-sequence-id-424626

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  19. pHAGE pGK MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid
    #215011
    Purpose
    Express a Mitophagy reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    pSu9 (Presequence of Fo-ATPase subunit 9)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Available Since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215011-plasmid-map-sequence-id-429274

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  21. pOpen-T3gene1

    Plasmid
    #165576
    Purpose
    DNA-dependent RNA polymerase derived from T3 bacteriophage that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    T3 RNA Polymerase
    Use
    Synthetic Biology
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165576-plasmid-map-sequence-id-340988

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  22. pSiM1

    Plasmid
    #199380
    Purpose
    Encodes dual cistronic membrane localized GCaMP6s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    GCaMP6s::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Available Since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199380-plasmid-map-sequence-id-391037

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  23. pSiM4

    Plasmid
    #199383
    Purpose
    Encodes dual cistronic membrane localized jGCaMP7f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    jGCaMP6f::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Available Since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199383-plasmid-map-sequence-id-391021

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  24. pSiM3

    Plasmid
    #199382
    Purpose
    Encodes dual cistronic membrane localized jGCaMP7s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    jGCaMP7s::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Available Since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199382-plasmid-map-sequence-id-391043

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  25. SnFR_S074

    Plasmid
    #182811
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Insert
    S074-CholineSnFR
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    182811-plasmid-map-sequence-id-358311

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  26. SnFR_S062

    Plasmid
    #182810
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Insert
    S062-CholineSnFR
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    182810-plasmid-map-sequence-id-358310

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  27. Ec holD-pET(3c)

    Plasmid
    #237236
    Purpose
    over-express E. coli holD (Pol III psi subunit)
    Depositor
    Michael O'Donnell
    Insert
    holD (psi) (holD E. coli)
    Expression
    Bacterial
    Available Since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237236-plasmid-map-sequence-id-469263

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  28. Ec holC-pET(3c)

    Plasmid
    #237235
    Purpose
    over-express E. coli holC (Pol III chi subunit)
    Depositor
    Michael O'Donnell
    Insert
    holC (chi) (holC E. coli)
    Expression
    Bacterial
    Available Since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237235-plasmid-map-sequence-id-469249

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  29. pET41-FeoB-C675S

    Plasmid
    #216756
    Purpose
    Investigation of the potential interaction of the FeoB Cys675 residue with ferrous iron
    Depositor
    Rietie Venter
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Amino acid C675 is substituted with S.
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    216756-plasmid-map-sequence-id-434497

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  30. pET41-FeoB-C429S

    Plasmid
    #216755
    Purpose
    Investigation of the potential interaction of the FeoB Cys429 residue with ferrous iron
    Depositor
    Rietie Venter
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Amino acid C429 is substituted with S.
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    216755-plasmid-map-sequence-id-434494

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  31. pET41-FeoB-D123N

    Plasmid
    #216754
    Purpose
    Production of a FeoB that is unable to bind GTP for a negative control in GTPases assays
    Depositor
    Rietie Venter
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Amino acid D123 is substituted with N.
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    216754-plasmid-map-sequence-id-434490

    Enlarge

  32. pSiM2

    Plasmid
    #199381
    Purpose
    Encodes dual cistronic membrane localized GCaMP6f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    GCaMP6f::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Available Since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199381-plasmid-map-sequence-id-391045

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  33. Pv-RNR-R2

    Plasmid
    #25589
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Pv-RNR-R2
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. Cavb3

    Plasmid
    #26574
    Depositor
    Diane Lipscombe
    Insert
    calcium channel beta subunit-III (Cacnb3 Rat)
    Expression
    Mammalian
    Available Since
    Nov. 29, 2010
    Availability
    Academic Institutions and Nonprofits only
    26574-plasmid-map-sequence-id-148124

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  35. pDSP33

    Plasmid
    #199375
    Purpose
    Encodes dual cistronic nuclear localized jGCaMP7f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::jGCaMP7f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Available Since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199375-plasmid-map-sequence-id-391013

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  36. pDSP31

    Plasmid
    #199373
    Purpose
    Encodes dual cistronic nuclear localized GCaMP6f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::GCaMP6f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Available Since
    April 25, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199373-plasmid-map-sequence-id-390590

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  37. pRV-TdTom-MCS

    Plasmid
    #193711
    Purpose
    Retroviral plasmid DNA for subcloning an oligonucleotide library encoding RNA barcodes
    Depositor
    Colin Akerman
    Type
    Empty backbone
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    193711-plasmid-map-sequence-id-387442

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  38. pDSP32

    Plasmid
    #199374
    Purpose
    Encodes dual cistronic nuclear localized jGCaMP7s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::jGCaMP7s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Available Since
    April 25, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199374-plasmid-map-sequence-id-390801

    Enlarge

  39. pDSP23

    Plasmid
    #199372
    Purpose
    Encodes dual cistronic nuclear localized GCaMP6s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::GCaMP6s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Available Since
    April 25, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199372-plasmid-map-sequence-id-390928

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  40. SnFR_V.7.1.2

    Plasmid
    #182809
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Insert
    V.7.1.2-CholineSnFR
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    182809-plasmid-map-sequence-id-358174

    Enlarge

Showing: 6841 - 6880 of 9862 results