Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 5,533 results for: PEP

Clear keywords
Showing: 5161 - 5190 of 5533 results

  1. GHSR-DuET

    Plasmid
    #213251
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GHSR (GHSR Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213251-plasmid-map-sequence-id-430901

    Enlarge

  2. pBZ210-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Ec86RT-NLS-T2A-mCherry

    Plasmid
    #170185
    Purpose
    Expression vector for a sgRNA against BFP and spCas9-Ec86 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Ec86 msr-msd region by SpeI and AvrII.
    Depositor
    Hunter Fraser
    Article
    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris
    Inserts
    Ec86 RT
    Retron Ec86 msr-msd
    BFP-to-GFP conversion donor template An
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Promoter
    CBh and hU6
    Available Since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    170185-plasmid-map-sequence-id-333571

    Enlarge

  4. GPR12-DuET

    Plasmid
    #213260
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPR12 (GPR12 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213260-plasmid-map-sequence-id-430810

    Enlarge

  5. mVenus-proα1(I)

    Plasmid
    #119845
    Purpose
    Expresses mouse Type I procollagen α1 chain (Col1a1) with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α1 chain (Col1a1 Mouse)
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Col1a1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available Since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119845-plasmid-map-sequence-id-233871

    Enlarge

  7. EDNRB-DuET

    Plasmid
    #213233
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    EDNRB (EDNRB Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213233-plasmid-map-sequence-id-430896

    Enlarge

  8. CX3C1-DuET

    Plasmid
    #213217
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CX3C1 (CX3CR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213217-plasmid-map-sequence-id-430779

    Enlarge

  9. pBZ202-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Sa163RT-NLS-T2A-mCherry

    Plasmid
    #170184
    Purpose
    Expression vector for a sgRNA against BFP and spCas9-Sa163 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Sa163 msr-msd region by SpeI and AvrII.
    Depositor
    Hunter Fraser
    Article
    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris
    Inserts
    Sa163 RT
    Retron Sa163 msr-msd
    BFP-to-GFP conversion donor template An
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Promoter
    CBh and hU6
    Available Since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    170184-plasmid-map-sequence-id-342530

    Enlarge

  10. PGK-HA-PGRN-LAMP1TM

    Plasmid
    #234873
    Purpose
    Lentiviral plasmid expressing HA-tagged human progranulin that is fused to the transmembrane domain and cytosolic tail of LAMP-1. Enables lysosomal delivery of progranulin without secretion.
    Depositor
    Andrew Arrant
    Article
    Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi
    Insert
    Progranulin (GRN Human)
    Use
    Lentiviral
    Tags
    HA tag and LAMP-1 transmembrane domain and cytoso…
    Expression
    Mammalian
    Promoter
    PGK
    Available Since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234873-plasmid-map-sequence-id-469050

    Enlarge

  11. GPRC5B-DuET

    Plasmid
    #213296
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPRC5B (GPRC5B Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213296-plasmid-map-sequence-id-430840

    Enlarge

  13. CRHR2-DuET

    Plasmid
    #213216
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CRHR2 (CRHR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213216-plasmid-map-sequence-id-430778

    Enlarge

  14. CCR4-DuET

    Plasmid
    #213200
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR4 (CCR4 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213200-plasmid-map-sequence-id-430763

    Enlarge

  15. OPRK1-DuET

    Plasmid
    #213359
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    OPRK1 (OPRK1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213359-plasmid-map-sequence-id-430881

    Enlarge

  16. mCerulean-proα1(I)

    Plasmid
    #119842
    Purpose
    Expresses mouse Type I procollagen α1 chain (Col1a1) with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α1 chain (Col1a1 Mouse)
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Col1a1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available Since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119842-plasmid-map-sequence-id-233869

    Enlarge

  17. CYSLTR1-DuET

    Plasmid
    #213225
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CYSLTR1 (CYSLTR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213225-plasmid-map-sequence-id-430787

    Enlarge

  18. pCEP4-HLA-c-myc-optiT1R3 ECD-MHC

    Plasmid
    #113961
    Purpose
    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain fused to a canonical
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R3 (TAS1R3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    residues 21-563 fused to a transmembrane tether
    Promoter
    cmv
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113961-plasmid-map-sequence-id-215291

    Enlarge

  20. AAV-hSyn-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

    Plasmid
    #177345
    Purpose
    AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from Synapsin promoter
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    dead hSaCas9
    Use
    AAV and CRISPR
    Tags
    3x GCN4 peptide, 3xHA, and NLS
    Expression
    Mammalian
    Mutation
    D10A, N580A
    Promoter
    Synapsin
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177345-plasmid-map-sequence-id-353619

    Enlarge

  23. pMMK ENTR 4 CMV GST mTagBFP

    Plasmid
    #206259
    Purpose
    ENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    GTS mTagBFP
    Use
    Multimate/gateway entr 4
    Tags
    BFGT1 (Golgi localisation peptide)
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206259-plasmid-map-sequence-id-406393

    Enlarge

  24. mApple-proα2(I)-L

    Plasmid
    #120161
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available Since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    120161-plasmid-map-sequence-id-233911

    Enlarge

  25. HTR1D-DuET

    Plasmid
    #213318
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HTR1D (HTR1D Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213318-plasmid-map-sequence-id-430855

    Enlarge

  26. pSems leader-ALFA-mXFP-TpoR

    Plasmid
    #222945
    Purpose
    Expression of TPOR with ALFAtag and monomeric XFP in mammalian cells
    Depositor
    Jacob Piehler
    Article
    Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:
    Insert
    Thrombopoietin receptor (26-635) (MPL Human)
    Tags
    ALFAtag, Ig k-chain leader sequence, and mXFP
    Expression
    Mammalian
    Mutation
    mXFP: tryptophan 66 to phenylalanine, TPOR: Signa…
    Promoter
    CMV
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    222945-plasmid-map-sequence-id-457424

    Enlarge

  27. pCJ207

    Plasmid
    #162680
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162680-plasmid-map-sequence-id-321455

    Enlarge

  28. HTR1B-DuET

    Plasmid
    #213317
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HTR1B (HTR1B Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213317-plasmid-map-sequence-id-430854

    Enlarge

Showing: 5161 - 5190 of 5533 results