We narrowed to 171,191 results for: addgene
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Plasmid#133233PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), sfGFP (pOGG037) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0380
Plasmid#133230PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA1_NANOG
Plasmid#64153PurposePhotoactivatable transcription system. Lentiviral expression of NANOG sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorAvailable SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shMki67
Plasmid#160958PurposeMki67 shRNA in pMKO.1 retroviral vectorDepositorAvailable SinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
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tet pLKO.1-shCSE1L v1 puro
Plasmid#192345PurposeLentiviral expression vector for an inducible shCSE1L v1DepositorInsertshCSE1L v1 (CSE1L Human)
UseLentiviralAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
tet pLKO.1-shCSE1L v2 puro
Plasmid#192346PurposeLentiviral expression vector for an inducible shCSE1L v2)DepositorInsertshCSE1L v2 (CSE1L Human)
UseLentiviralAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-iPuro-L3 #BGV205
Plasmid#48987PurposeSupplies the selection marker (puromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInsertpuromycin resistance
UseGateway cloning vectorTagsIRES (weak)PromoterNAAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
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(517) SB KRAB-SadCas9 U6-gStop
Plasmid#163023PurposeSleeping Beauty TET-On expression of CRISPRi with gSTOP gRNADepositorInsertMammalian codon-optimized SadCas9
UseTransposonTagsKRAB and myc NLSExpressionMammalianPromoterTet-OnAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
ADARB1_pCSdest
Plasmid#53939DepositorAvailable SinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCRISPR_RAF1_94
Plasmid#111826PurposeKnockout Raf1DepositorInsertgRNA targeting RAF1 94-114 (RAF1 Human)
UseCRISPRTagsmCherryExpressionBacterial and MammalianPromoterU6Available SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pyc128m
Plasmid#158778Purposesoluble GCaMP expressionDepositorInsertGCaMP6m
UseAAVAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Atoh1ER-IRES-GFP
Plasmid#33335DepositorAvailable SinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
BirA_BVES untagged
Plasmid#196581Purposeexpresses BirADepositorInsertBirA_Ecoli
UseBaculovirus expressionAvailable SinceJune 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPLN-hFluc-DM-eGFP-KDEL
Plasmid#177728PurposeDM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminusDepositorInserthFlucDM
TagsKDEL, Prolactin Signal Sequence, and eGFPExpressionBacterial and MammalianMutationR188Q, R261Q (Destabilized Mutant)Available SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pgRNA_hMECP2_promoter_No4
Plasmid#194899PurposeLentiviral construct with mCherry and Puro cassettes to express sgRNA-4 targeting the promoter of human MECP2DepositorInsertsgRNA targeting human MECP2 promoter No.4
UseLentiviralExpressionMammalianPromoterU6Available SinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAG-RORb2-IRES-GFPd2
Plasmid#73998PurposePlasmid expressing RORb2-IRES-GFPd2DepositorAvailable SinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_BET1
Plasmid#111683PurposeMAC-tagged gene expressionDepositorInsertBET1 (BET1 Human)
ExpressionMammalianAvailable SinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_FBRL
Plasmid#111686PurposeMAC-tagged gene expressionDepositorInsertFBRL (FBL Human)
ExpressionMammalianAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
ITSN1_pCSdest
Plasmid#53800DepositorAvailable SinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDK197
Plasmid#134189PurposeRetroviral plasmid expressing LAP-Clasp C terminusDepositorAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABE human IRS-1 CAAX
Plasmid#11237DepositorInsertIRS-1 (IRS1 Human)
UseRetroviralTagsCAAXExpressionMammalianMutationC-terminal prenylation motif makes IRS-1 localize…Available SinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLpC-Zipp.-L-IFP-F[1]+Plum (mammalian)
Plasmid#52907PurposeBinary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[1]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI. Confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[1]+Plum
UseRetroviralTagsL-IFP-F[1]ExpressionMammalianPromoterUnknownAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
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BACH1_pCSdest
Plasmid#53820DepositorAvailable SinceSept. 9, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
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TET-pLKO.1 PURO shId2 #2
Plasmid#83090PurposeLentiviral shRNA vector for inducible knockdown of mouse Id2DepositorAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-eGFP-L3 #BGV201
Plasmid#48979PurposeSupplies the selection marker (eGFP) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInserteGFP
UseGateway cloning vectorTagsIRES (strong)PromoterNAAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSN69
Plasmid#100125Purposeexpresses putative cMYC DBD with a HA tagDepositorInsertmCerulean-P2A-putative cMYC DBD with a HA tag (MYC Human)
UseLentiviralExpressionMammalianMutationInsert corresponds to the DBD of cMYCAvailable SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmem53-V5
Plasmid#175142PurposeLentiviral expression of mouse Tmem53-V5DepositorAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATP5J_pCSdest
Plasmid#53854DepositorAvailable SinceSept. 9, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBEG R2-iNeo-L3 #BGV207
Plasmid#48986PurposeSupplies the selection marker (neomycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInsertneomycin resistance
UseGateway cloning vectorTagsIRES (weak)PromoterNAAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dll4CE2-P1Egfp
Plasmid#90147Purposecontains dll4 enhancer 2 sequence, and dll4 specific gene promoter driving expression of eGFPDepositorAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMB1052:miniTol2-U6-(empty)SaCas9_gRNA-CAG-Cre-P2A-NeoR-WPRE
Plasmid#168108PurposeTol2 transposon vector of empty U6-S.aureus Cas9 gRNA cassette and CAG-driven Cre-NeoRDepositorTypeEmpty backboneUseCRISPR and Cre/LoxAvailable SinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT S6K (E389-deltaCT)
Plasmid#58507PurposeGateway entry vector for an inducible HA-tagged rat S6K1-E389-deltaCTDepositorAvailable SinceAug. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shPrc1
Plasmid#160959PurposePrc1 shRNA in pMKO.1 retroviral vectorDepositorAvailable SinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
LIPI_pCSdest
Plasmid#53849DepositorAvailable SinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits