We narrowed to 18,423 results for: Met

1. Str-KDEL_ManII-SBP-EGFP

   Plasmid

   #65252

   Purpose

   synchronize trafficking of ManII from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin-KDEL and truncated Mannosidase II
   

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. mEmerald-PDHA1-N-10

   Plasmid

   #54225

   Purpose

   Localization: Mitochondria, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

   Insert

   PDHA1 (PDHA1 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pFGC5941-PacI-ath-STTM165/166-48nt

   Plasmid

   #84091

   Purpose

   target miRNA165/166 for destruction in Arabidopsis thaliana

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Insert

   STTM165/166
   

   Expression

   Plant

   Promoter

   2x35S

   Available Since

   Nov. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. PH-PLCD1_mScarlet_IRES_SYFP2_PH_N1

   Plasmid

   #85069

   Purpose

   Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   PH-PLCD1 (PLCD1 Human)
   

   Tags

   IRES-sYFP2-PH and mScarlet

   Expression

   Mammalian

   Mutation

   PLCD aa 1–170

   Promoter

   CMV

   Available Since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

   Plasmid

   #84247

   Purpose

   Expresses direct fusion VPR-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   VPR-tagBFP-Sp dCas9
   Puro
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), HA tag, VPR, and tagBFP

   Expression

   Mammalian

   Promoter

   CAG and PGK

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pST1374-N-NLS-flag-linker-Cas9-H840A

   Plasmid

   #51129

   Purpose

   Cas9 nickase optimized for nuclear import.

   Depositor

   Xingxu Huang

   Article

   Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

   Insert

   Cas9 nickase
   

   Use

   CRISPR

   Tags

   Flag and NLS

   Expression

   Mammalian

   Mutation

   H840A

   Promoter

   CMV

   Available Since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3xFLAG-dCas9/pMSCVhyg

   Plasmid

   #134323

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6_(GLuc)_INT[3xMS2_SL]

   Plasmid

   #68426

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing three MS2 Stem-loops
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCIB1(deltaNLS)-pmGFP

   Plasmid

   #28240

   Purpose

   Expresses CIB1 fused to plasma membrane-localized EGFP

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CIB1(deltaNLS)-pmGFP (CIB1 Mustard Weed)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   NLS sequences mutated

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. Actin Binding Domain Tensionless Control

    Plasmid

    #215371

    Purpose

    Expresses Actin Binding Domain Tensionless Control (ABDTL), composed of Ftractin-Linker-TSMod. This is the unloaded control for Actin Binding Domain Tension Sensor (ABDTS).

    Depositor

    Brenton Hoffman

    Article

    Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    Actin Binding Domain Tensionless Control
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. mTagRFP-T-H2B-6

    Plasmid

    #58019

    Purpose

    Localization: Nucleus/Histones, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    H2B (H2BC11 Human)
    

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. 2xCBX7CD-IFP2.0C

    Plasmid

    #215754

    Purpose

    Expresses double domain of CBX7 chromo domain fused to C-terminal part of IFP2.0 for H3K27me3 detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    2xCBX7CD-IFP2.0C
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBR_attB(C31)_FRT

    Plasmid

    #183761

    Purpose

    phiC31-specific donor plasmid for the cloning of payloads <20 kb

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. AF1521-c002

    Plasmid

    #173109

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    KH-M1-L192

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. AF1521-c003

    Plasmid

    #173111

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))
    

    Tags

    Nanoluc

    Expression

    Bacterial

    Mutation

    KH-M1-L192

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Brp-T2A-QF2w-Hsp70-3XP3-dsRed

    Plasmid

    #141094

    Purpose

    Template plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.

    Depositor

    Carolyn McBride

    Article

    Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

    Insert

    bruchpilot
    

    Use

    CRISPR

    Expression

    Insect

    Mutation

    coding sequence downstream of CRISPR cut site, sy…

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSFDuet-1_hGH

    Plasmid

    #182410

    Purpose

    Bacterial expression of human growth hormone

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    hGH (GH1 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Yellow Cameleon-Nano15/pBig

    Plasmid

    #51962

    Purpose

    Expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM, in Dictyostelium

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano15
    

    Expression

    Bacterial

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETDuet-1_VNp-mNeongreen_CydAB-mCherry

    Plasmid

    #182391

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen and cydAB-mCherry fusions

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-mNeongreen-His
    cydAB-mCherry
    

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-CT3G-CERP2-MG-IRES2-mCherry

    Plasmid

    #175507

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mCherry (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G-mCMV

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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