We narrowed to 18,263 results for: Met;

1. PB-CT3G-CERP2-MG-IRES2-mCherry

   Plasmid

   #175507

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mCherry (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-mCMV

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. RNF146-c005

   Plasmid

   #173091

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   RNF146 (RNF146 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   R99-L183, codon optimized

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH_Str-KDEL_ManII-SBP-EGFP

   Plasmid

   #65258

   Purpose

   synchronize trafficking of ManII from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin-KDEL and truncated Mannosidase II
   

   Use

   Lentiviral

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21-pelB-LaM-3

   Plasmid

   #172769

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-3
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-Map7-fulllength

   Plasmid

   #46076

   Depositor

   Edgar Gomes

   Article

   Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

   Insert

   Map7-fulllength (Map7 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. cmv-sfGFP-GB1-scAB-ubc-nls-pcp-12xSunTag(SRv1.2-2P)

   Plasmid

   #185802

   Purpose

   one plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.2

   Depositor

   Robin Lee

   Article

   Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

   Insert

   cmv-sfGFP-GB1-scAB--ubc-nls-pcp-12xSunTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Burden Monitor phi80 version

   Plasmid

   #66074

   Purpose

   The burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cell

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. GDAP2-c005

   Plasmid

   #173110

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   GDAP2 (GDAP2 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   M1-N231

   Promoter

   Available since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)

   Plasmid

   #83947

   Purpose

   gRNA cassette-carrying vector with natMX marker

   Depositor

   Irina Borodina

   Article

   Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

   Insert

   gRNA targeting ADE2 gene
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFD115

    Plasmid

    #124768

    Purpose

    pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

    Depositor

    David Bikard

    Article

    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

    Inserts

    Ptet
    cas9
    term PpflB sgRNA (BsaI)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ptet

    Available since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-pelB-LaG-30

    Plasmid

    #172754

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-30
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. mRuby2-Zyxin-6

    Plasmid

    #55917

    Purpose

    Localization: Focal Adhesions, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Zyxin (ZYX Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. Clover-PMP-N-10

    Plasmid

    #56305

    Purpose

    Localization: Peroxisomes, Excitation: 505, Emission: 515

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    PMP (PXMP2 Human)
    

    Use

    Tags

    mClover2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. anti-Notch2_B6-pBIOCAM5

    Plasmid

    #39347

    Depositor

    John McCafferty

    Article

    Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

    Insert

    scFv-Fc-fusion specific for Notch2 (clone B6) (NOTCH2 Human)
    

    Use

    Tags

    3xFLAG, 6xHis, and human Fc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNCS-mCardinal2

    Plasmid

    #52631

    Purpose

    bacterial expression vector with far-red FP mCardinal2

    Depositor

    Michael Lin

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    mCardinal2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21-pelB-LaM-8

    Plasmid

    #172772

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-8
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR223_HcRed

    Plasmid

    #25892

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    hcRed
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSB700 lenti gRNA Cerulean-zhang2.0 Weiss full

    Plasmid

    #79383

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    Zhang 2.0 + scaffold
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDS275

    Plasmid

    #34972

    Purpose

    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A mutations).

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T406A, T407A, I532A

    Promoter

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMBA333

    Plasmid

    #214745

    Purpose

    Medium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23119-RiboJ-RBS(21992)-gfpmut3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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