We narrowed to 6,961 results for: RAP

1. pLPC-FLAG.Mm.EPC2

   Plasmid

   #184649

   Purpose

   Retroviral expression of mouse EPC2

   Depositor

   Agnel Sfeir

   Article

   Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

   Insert

   Epc2 (Epc2 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLPC-FLAG.Mm.EPC1

   Plasmid

   #184648

   Purpose

   Retroviral expression of mouse EPC1

   Depositor

   Agnel Sfeir

   Article

   Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

   Insert

   EPC1 (Epc1 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-fDIO-pAce-Kv2.1PR

   Plasmid

   #195535

   Purpose

   Flp recombinase-dependent green fluorescent, positive response-polarity voltage indicator; soma-targeted

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   pAce-Kv2.1 proximal restriction sequence
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Ace-mNeon 78K, 81D, 92N, 178F; SY linker

   Promoter

   CAG

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-G88P7-rM3Ds-2A-EYFP

   Plasmid

   #213970

   Purpose

   AAV vector with G88P7 promoter in order to induce robust expression of rM3Ds in MSNs

   Depositor

   Zhonghua Lu

   Article

   Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

   Insert

   rM3Ds
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   G88P7

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. ABE8.20-d

   Plasmid

   #136301

   Purpose

   expresses ABE8.20-d in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.20-d
   

   Use

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   CMV

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSCIP-hCD69-TdTomato

   Plasmid

   #154095

   Purpose

   Self-Cutting and Integrating CRISPR backbone targeting human CD69 promoter with TdTomato reporter transgene

   Depositor

   Scott McComb

   Article

   Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

   Inserts

   [5HA]-P2A-tdTomato-[3HA]
   hCD69 targeting sgRNA - AGCTCTTTGCATCCGGAGAG
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSECC

   Plasmid

   #60820

   Purpose

   3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre

   Depositor

   Tyler Jacks

   Article

   Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature

   Inserts

   Cas9
   Cre
   

   Use

   CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   BsmBI site eliminated by C->A; E308G

   Promoter

   EFS and EFS (after Cas9-2A)

   Available since

   Nov. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. Mito-mGreenLantern

   Plasmid

   #164461

   Purpose

   Localization of mGreenLantern fluorescent protein to mitochondria

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Use

   Tags

   Human COX8a first 29 amino acids

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CMV

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ABE8.17-m

   Plasmid

   #136298

   Purpose

   expresses ABE8.17-m in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.17-m
   

   Use

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMV306DIG13+FFlucRT

    Plasmid

    #49998

    Purpose

    Integrase removed to increase stability of the plasmid in mycobacteria

    Depositor

    Brian Robertson

    Article

    Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2

    Insert

    G13 promoter + Firefly luciferase Red Thermostable
    

    Use

    Mycobacteria integrating vector

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for Mycobacterium tuberculosis wi…

    Promoter

    G13

    Available since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Str-Ii_SBP-EGFP-Golgin84

    Plasmid

    #65303

    Purpose

    synchronize trafficking of Golgin-84 from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Golgin-84 (GOLGA5 Human)
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-DIO-pAceR-Kv2.1PR

    Plasmid

    #195534

    Purpose

    Cre-dependent red fluorescent, positive response-polarity voltage indicator; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    pAceR-Kv2.1 proximal restriction sequence
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    VARNAM 78E, 81D, 92N; SI linker

    Promoter

    CAG

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-DIO-Ace-mNeon2-Kv2.1PR

    Plasmid

    #195532

    Purpose

    Cre-dependent expression of a green fluorescent, negative response-polarity voltage indicator; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    Ace-mNeon2-Kv2.1 proximal restriction sequence
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Ace-mNeon 81S; SY linker

    Promoter

    CAG

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pW34

    Plasmid

    #158210

    Purpose

    LuxCDABE operon (R6K ori) under T7, in loxP and lox5171 sites.

    Depositor

    Yasuo Yoshikuni

    Article

    Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

    Insert

    LuxABCDE
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR_PGK_SNIPR_Hinge Notch SNIPR (HNF1A, ALPPL2 scFv)

    Plasmid

    #188385

    Purpose

    Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGFP-HBO1-G485A pc2201

    Plasmid

    #167570

    Purpose

    The human HBO1 is fused to C-terminal GFP of EGFP-C1 (Clontech)

    Depositor

    Cristina Cardoso

    Article

    Heinz et al Epigenetics Chromatin. 2019 Mar 14;12(1)

    Insert

    histone acetyltransferase 1 (KAT7 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G485A

    Promoter

    CMV

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGG-B1

    Plasmid

    #78504

    Purpose

    Trans plasmid for production of recombinant AAV-B1 vectors. Plasmid backbone may yield higher titers than pAR-B1 and encodes AAV2 Rep and AAV-B1 capsid

    Depositor

    Miguel Sena-Esteves

    Article

    Choudhury et al Mol Ther. 2016 Apr 27. doi: 10.1038/mt.2

    Inserts

    AAV2 Rep
    AAV-B1 capsid
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-FLuc

    Plasmid

    #83281

    Purpose

    recombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)

    Depositor

    Mark Kay

    Article

    Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

    Insert

    Firefly Luciferase
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO.1-TRC.mKO2

    Plasmid

    #85208

    Purpose

    Backbone for lentiviral gene silencing with monomeric Kusabira-Orange2 expression

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CLXSN 5myc-CDC6-WT

    Plasmid

    #109332

    Purpose

    express tagged Cdc6 WT

    Depositor

    Jean Cook

    Article

    Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

    Insert

    CDC6 (CDC6 Human)
    

    Use

    Retroviral

    Tags

    5x Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTP261

    Plasmid

    #104166

    Purpose

    Bacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labeling

    Depositor

    Dirk Görlich

    Article

    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

    Insert

    Anti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)
    

    Use

    Tags

    14x Histidine tag and NEDD8 from Brachypodium dis…

    Expression

    Bacterial

    Mutation

    1x Cysteine located at bps 493-495 in full sequen…

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pmiRFP2-Actin-C-18

    Plasmid

    #178965

    Purpose

    Expresses the fusion protein of miRFP2-Actin-C in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    miRFP2-actin
    

    Use

    Tags

    actin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMSCVpuro-Flag-cMyc T58A

    Plasmid

    #20076

    Depositor

    Juan Belmonte

    Article

    Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

    Insert

    cMyc (MYC Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T58A

    Promoter

    Available since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMV306G13+FFlucRT

    Plasmid

    #49997

    Purpose

    Integrating vector with an improved Red-shifted Thermostable Firefly Luciferase

    Depositor

    Brian Robertson

    Article

    Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2

    Insert

    G13 promoter + Firefly luciferase Red Thermostable
    

    Use

    Mycobacteria integrating vector

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for Mycobacterium tuberculosis wi…

    Promoter

    G13 from M. marinum

    Available since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28-MHL: nsp16_SARS-CoV-2

    Plasmid

    #167850

    Purpose

    Bacterial expression of the SARS-CoV-2 protein nsp16

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp16
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pET28-MHL: nsp10_SARS-CoV-2

    Plasmid

    #167851

    Purpose

    Bacterial expression of the SARS-CoV-2 protein nsp10

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp10
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. alpha catenin conformation sensor

    Plasmid

    #71709

    Purpose

    This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.

    Depositor

    Deborah Leckband

    Article

    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

    Insert

    alpha-catenin FRET Sensor
    

    Use

    Tags

    ECFP and YPET

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMGS46 (ARF16-PB1-HA-P2A-OsTIR1)

    Plasmid

    #126580

    Purpose

    A repair construct to express ARF16-PB1-HA and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    OsARF16-PB1 domain
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ER-mGreenLantern

    Plasmid

    #164463

    Purpose

    Localization and retention of mGreenLantern fluorescent protein in the ER

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-hSyn-GRAB_NEmut

    Plasmid

    #123310

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neurons

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Has Service

    AAV Retrograde and AAV9

    Insert

    GPCR activation based NE control sensor GRAB_NEmut
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge