We narrowed to 9,393 results for: Coli

1. ReU_pET29b

   Plasmid

   #215426

   Purpose

   Expression construct for ReU gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   ReU
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ThcCut2 _pET29b

   Plasmid

   #215430

   Purpose

   Expression construct for ThcCut2 gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   ThcCut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TtA_pET29b

   Plasmid

   #215432

   Purpose

   Expression construct for TtA gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   TtA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MsmegA _pET29b

   Plasmid

   #215411

   Purpose

   Expression construct for MsmegA gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   MsmegA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Nc-NylC_pET28a

   Plasmid

   #215412

   Purpose

   Expression construct for Nc-NylC gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   Nc-NylC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AoC_pET29b

   Plasmid

   #215402

   Purpose

   Expression construct for AoC gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   AoC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-Duet_OPTN-thrombin-GST

   Plasmid

   #216843

   Purpose

   For expression in E. coli of OPTN (Internal ref. SMC1789)

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   Optineurin (OPTN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGG-TurboID-PTS1

   Plasmid

   #209398

   Purpose

   Transiently expressing TurboID fused with a peroxisomal targeting signal type I (PTS1) in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   3XHA-TurboID-PTS1
   

   Use

   Tags

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGG-GTS-TurboID-GFP

   Plasmid

   #209405

   Purpose

   Transiently expressing and visualizing the subcellular localization of TurboID fused with a Golgi targeting sequence (GTS) in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   GTS-3XHA-TurboID
   

   Use

   Tags

   GFP

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGG-mTP-TurboID

    Plasmid

    #209396

    Purpose

    Transiently expressing TurboID fused with a Mitochondrial transit peptide (mTP) in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    mTP-3XHA-TurboID
    

    Use

    Tags

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGG-mTP-TurboID-GFP

    Plasmid

    #209404

    Purpose

    Transiently expressing and visualizing the subcellular localization of TurboID fused with a Mitochondrial transit peptide (mTP) in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    mTP-3XHA-TurboID
    

    Use

    Tags

    GFP

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 1BR9-BFP

    Plasmid

    #202054

    Purpose

    E. coli expression vector for N-ter GFP11 and C-ter R9 tagged tagBFP.

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    BFP
    

    Use

    Tags

    6xHis, GFP11, and R9

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 1BR9-Lifeact

    Plasmid

    #193863

    Purpose

    E. coli expression vector for N-ter GFP11 and C-ter R9 tagged lifeact peptide.

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    Lifeact
    

    Use

    Tags

    6xHis, GFP11, and R9

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET-20b_HtrA2(delta(133))_S306A_6His

    Plasmid

    #197975

    Purpose

    E. coli expression of HtrA2(delta133)-6His

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    delta(1-133), S306A

    Promoter

    Available since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pnEA-NpM-HsNot4_400-424_AG

    Plasmid

    #148790

    Purpose

    E. coli of HsNot4_400-424

    Depositor

    Elisa Izaurralde

    Article

    Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

    Insert

    HsNot4_400-424 (CNOT4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. P2061

    Plasmid

    #186299

    Purpose

    Expresses LexA-HBD-B112 under the control of pAct1 and Cas9 under the control of LexA-HBD-B112+Beta-estradiol inducible promoter

    Depositor

    Joerg Stelling

    Article

    Azizoglu et al Curr Protoc. 2023 Jan;3(1):e647. doi: 10

    Inserts

    LexA-HBD-B112
    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    2xLexop-minimalCyc1 and PACT1

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pXD70Tet(DadRmt5)-N-3xFLAG-DadR(ΔDBD)

    Plasmid

    #191641

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(ΔDBD) complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    DadR
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    ΔDBD

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXD70Tet(DadRmt6)-N-3xFLAG-DadR(ΔTM)

    Plasmid

    #191642

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(ΔTM) complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    DadR
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    ΔTM

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pXD70Tet(DadRmt7)-N-3xFLAG-DadR(linker)

    Plasmid

    #191643

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(linker region shuffled) complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    DadR
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Linker region sequence shuffled

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXD70Tet(DadH)-Pdadh-DadhABC(A2)

    Plasmid

    #191644

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    Dopamine dehydroxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET29a(+)::9xhis-Tse5

    Plasmid

    #192962

    Purpose

    Heterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CT

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    Tse5
    

    Use

    Tags

    9xhis + TEV tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET-PLS-KP7_rpoA-U

    Plasmid

    #190974

    Purpose

    Test the U-to-C editing activity of KP7 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP7
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET-PLS-KP2-E67A_rpoA-U

    Plasmid

    #190959

    Purpose

    Test the U-to-C editing activity of KP2:E67A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:E67A

    Promoter

    T7

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-PLS-KP6-A66S_rpoA-C

    Plasmid

    #190968

    Purpose

    Test the C-to-U editing activity of KP6:A66S on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:A66S

    Promoter

    T7

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-PLS-KP1_rpoA-U

    Plasmid

    #190955

    Purpose

    Test the U-to-C editing activity of KP1 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP1
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-PLS-KP2-A66S_rpoA-U

    Plasmid

    #190957

    Purpose

    Test the U-to-C editing activity of KP2:A66S on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:A66S

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-PLS-KP3-A66S_rpoA-U

    Plasmid

    #190961

    Purpose

    Test the U-to-C editing activity of KP3:A66S on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:A66S

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET-PLS-KP3-E67A_rpoA-U

    Plasmid

    #190963

    Purpose

    Test the U-to-C editing activity of KP3:E67A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:E67A

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-PLS-KP4_rpoA-U

    Plasmid

    #190964

    Purpose

    Test the U-to-C editing activity of KP4 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP4
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET-PLS-KP6-E67A_rpoA-C

    Plasmid

    #190972

    Purpose

    Test the C-to-U editing activity of KP6:E67A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:E67A

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET-PLS-KP5_rpoA-U

    Plasmid

    #190965

    Purpose

    Test the U-to-C editing activity of KP5 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP5
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. VAMP8

    Plasmid

    #186689

    Purpose

    Expresses VAMP8 in E. Coli

    Depositor

    Axel Brunger

    Article

    Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

    Insert

    VAMP8
    

    Use

    Tags

    3xFLAG, GST, TEV, and Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Stx3

    Plasmid

    #186685

    Purpose

    Expresses Syntaxin 3 in E. Coli

    Depositor

    Axel Brunger

    Article

    Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

    Insert

    Syntaxin 3
    

    Use

    Tags

    6 his tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEUK035

    Plasmid

    #180827

    Purpose

    T7-inducible expression construct to produce SwGdmB (HY78_09235) in E. coli

    Depositor

    Gregg Beckham

    Article

    Bleem et al Chem Catalysis, 2022

    Insert

    SwGdmB
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEUK036

    Plasmid

    #180828

    Purpose

    T7-inducible expression construct to produce CnGdmA (CNE_RS35785) in E. coli

    Depositor

    Gregg Beckham

    Article

    Bleem et al Chem Catalysis, 2022

    Insert

    CnGdmA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEUK066

    Plasmid

    #180830

    Purpose

    T7-inducible expression construct to produce NaGdmA (SARO_RS07455) in E. coli

    Depositor

    Gregg Beckham

    Article

    Bleem et al Chem Catalysis, 2022

    Insert

    NaGdmA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRSET-TorA-6xHis-M13-pHCountdown-Calmodulin

    Plasmid

    #182305

    Purpose

    E. coli plasmid encoding a fusion between pH-Countdown and the calcium-binding domains of GECO 1.2 with a hexahistidine tag, targeted to the periplasm via the TorA presequence.

    Depositor

    Andrew York

    Article

    Seidel et al (2021), doi: 10.5281/zenodo.5810930

    Insert

    pH-Countdown insertion into GECO 1.2 calcium sensing domains
    

    Use

    Tags

    A modified TorA periplasmic targeting sequence pl…

    Expression

    Bacterial

    Mutation

    Spontaneous mutation of calmodulin's penulti…

    Promoter

    t7

    Available since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. MBP_Lims (MBP_LIM1234)

    Plasmid

    #182042

    Purpose

    For expression of human paxillin LIM domains 1-4 with N-terminal GST fusion protein in E. coli.

    Depositor

    Vesa Hytönen

    Article

    Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

    Insert

    LIM1234 (PXN Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    LIM domains 1-4 only

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. GST_LIM1224

    Plasmid

    #182041

    Purpose

    For expression of human paxillin LIM domains 1, 2, 2 and 4 with N-terminal GST fusion protein in E. coli.

    Depositor

    Vesa Hytönen

    Article

    Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

    Insert

    LIM1224 (PXN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    LIM domains 1, 2, 2 and 4

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. GST_LIM1334

    Plasmid

    #182040

    Purpose

    For expression of human paxillin LIM domains 1, 3, 3 and 4 with N-terminal GST fusion protein in E. coli.

    Depositor

    Vesa Hytönen

    Article

    Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

    Insert

    LIM1334 (PXN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    LIM domains 1, 3, 3 and 4

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge